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TAE Buffer
From 2009.igem.org
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(New page: {{UIUC09Temp}} {{Illinois}} {{IllExpNav}} [https://2009.igem.org/Team:Illinois/Protocols#Recipes Back to Protocols page] <html> <div id="uicontentbox"> </html> ==1x TAE Buffer== *242g Tri...) |
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*57.1mL glacial acetic acid | *57.1mL glacial acetic acid | ||
*100mL 0.5M EDTA (pH 8.0) | *100mL 0.5M EDTA (pH 8.0) | ||
| - | * | + | *dH<sub>2</sub>O |
| - | + | First, prepare a concentrated 50x stock solution of TAE buffer. To do this, dissolve Tris base in 750mL of deionized water. Add the acetic acid and EDTA, and adjust the volume to 1L by adding water. The final pH of the 50x TAE buffer should be about 8.5. To make the 1x TAE working buffer, add 49 parts of deionized water to 1 part of 50x TAE buffer. | |
<html> | <html> | ||
</div> | </div> | ||
</html> | </html> | ||
{{IllinoisBottomNav}} | {{IllinoisBottomNav}} | ||
Latest revision as of 20:25, 6 August 2009
1x TAE Buffer
- 242g Tris base
- 57.1mL glacial acetic acid
- 100mL 0.5M EDTA (pH 8.0)
- dH2O
First, prepare a concentrated 50x stock solution of TAE buffer. To do this, dissolve Tris base in 750mL of deionized water. Add the acetic acid and EDTA, and adjust the volume to 1L by adding water. The final pH of the 50x TAE buffer should be about 8.5. To make the 1x TAE working buffer, add 49 parts of deionized water to 1 part of 50x TAE buffer.
