Team:Paris/Freezer PCR products

From 2009.igem.org

(Difference between revisions)
(→PCR products)
 
(16 intermediate revisions not shown)
Line 1: Line 1:
 +
<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Freezer#top | Freezer]] > [[Team:Paris/Freezer_PCR_products#bottom | PCR Products]]
{{Template:Paris2009}}
{{Template:Paris2009}}
{{Template:Paris2009_menu}}
{{Template:Paris2009_menu}}
-
==Freezer==
 
-
<center>
+
== PCR products ==
-
[[Team:Paris/Freezer_Primers#Freezer | Primers]] -
+
<html>
-
[[Team:Paris/Freezer_PCR_products#Freezer | PCR products]] - 
+
<style type="text/css">
-
[[Team:Paris/Freezer_Plasmids#Freezer | Plasmids]] -
+
#left-side {
-
[[Team:Paris/Freezer_Digestion_Products#Freezer | Digestion Products]] -
+
    position: absolute;
-
[[Team:Paris/Freezer_Unexpected_digestion_results#Freezer | Unexpected digestion results]] -
+
    height: 23px;
-
[[Team:Paris/Freezer_Strains_(Glycerol stock)#Freezer | Strains (Glycerol stock)]]
+
    width: 30px;
 +
    top: 0px;
 +
    left: 10px;
 +
    margin-top:10px;
 +
    padding-top: 7px;
 +
    background: url(https://static.igem.org/mediawiki/2009/1/1b/Left_menu_pari.png);
 +
    z-index:4;
 +
}
 +
 
 +
#middle-side {
 +
    height: 25px;
 +
    width: 650px;
 +
    position: absolute;
 +
    top: 0px;
 +
    left: 30px;
 +
    margin-top:10px;
 +
    padding-top: 5px;
 +
    background: #dadada;
 +
    z-index:5;
 +
}
 +
 
 +
#right-side {
 +
    position: absolute;
 +
    height: 23px;
 +
    width: 30px;
 +
    margin-top:10px;
 +
    padding-top: 7px;
 +
    top: 0px;
 +
    left: 670px;
 +
    background: url(https://static.igem.org/mediawiki/2009/4/40/Right_menu_paris.png);
 +
    z-index:4;
 +
}
 +
 
 +
a.menu_sub {
 +
    padding-left: 7px;
 +
    padding-right: 7px;
 +
}
 +
 
 +
a.menu_sub_active {
 +
    padding-left: 7px;
 +
    padding-right: 7px;
 +
    color:#b0310e;
 +
    font-weight:bold;
 +
}
 +
</style>
 +
<div id="left-side"></div>
 +
<div id="middle-side"><center>
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer#bottom"> Main </a>|
 +
<a class="menu_sub_active" href="https://2009.igem.org/Team:Paris/Freezer_PCR_products#bottom"> PCR Products</a>|
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Plasmids#bottom"> Plasmids</a>|
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Digestion_Products#bottom"> Digestion Products</a>|
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#bottom"> Strains (Glycerol stock)</a>|
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/constructions#bottom"> Constructions in progress</a>
</center>
</center>
 +
</div>
 +
 +
<div id="right-side"></div>
 +
 +
</html>
 +
<br>
<br>
{|
{|
Line 26: Line 84:
|width=10% style="background: #FF0066;"| Chloranphenicol
|width=10% style="background: #FF0066;"| Chloranphenicol
|}
|}
-
<html>
 
-
</div>
 
-
<div id="paris_content_boxtop">
 
-
</div>
 
-
<div id="paris_content">
 
-
</html>
 
-
== PCR products ==
+
 
 +
 
{|
{|
Line 39: Line 92:
! colspan="11" style="background: #660099;"|PCR products
! colspan="11" style="background: #660099;"|PCR products
|- style="background: #0d3e99; text-align: center; color:white;"
|- style="background: #0d3e99; text-align: center; color:white;"
-
|width=5%| Number
+
|width=5%| Ref
|width=10%| Name
|width=10%| Name
-
|width=10%| Oligo F
+
|width=5%| Oligo Fw
-
|width=10%| Oligo R
+
|width=5%| Oligo Rev
-
|width=5%| Tm
+
|width=10%| Tm used(Tm cal.) (°C)
|width=10%| Matrix
|width=10%| Matrix
-
|width=10%| Length
+
|width=10%| Length (bp)
|width=25%| Comments
|width=25%| Comments
|width=5%| Quantity
|width=5%| Quantity
-
|width=5%| []ng/µL
+
|width=5%| [ ] (ng/µL)
|width=5%| Date
|width=5%| Date
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|width=5%| A1 <span/ id="A1">
|width=5%| A1 <span/ id="A1">
 +
|width=10%| Tg3p_fusion N term
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O18 O18 ] 
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O17 O17 ]
 +
|width=5%| ? (63.5)
|width=10%| ?
|width=10%| ?
-
|width=10%| [O18] Tg3p_fusion_Fw 
+
|width=10%| 274
-
|width=10%| [O17] Tg3p_Rw
+
|width=25%| Amplification of N-term domain of M13's g3p
|width=5%| ?
|width=5%| ?
-
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
+
|width=5%| ?
-
|width=10%| 274bp
+
|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05]
-
|width=25%| Amplification of N-term domain of M13's g3p
+
-
|width=5%| [https://2009.igem.org/Team:Paris/Fridge F4]
+
-
|width=5%| ? ng/µL
+
-
|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#A1 08/05]
+
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|width=5%| A2 <span/ id="A2">
|width=5%| A2 <span/ id="A2">
-
|width=10%| ?
+
|width=10%| TE3_fusion N term
-
|width=10%| [O15] TE3_fusion_Fw
+
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O15 O15 ]  
-
|width=10%| [O16] TE3_Rw
+
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O16 O16 ]
|width=5%| ?
|width=5%| ?
-
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
+
|width=10%| ???
-
|width=10%| 1036bp
+
|width=10%| 1036
|width=25%| Amplification of N-term domain of colicin E3
|width=25%| Amplification of N-term domain of colicin E3
|width=5%| ?
|width=5%| ?
-
|width=5%| ? ng/µL
+
|width=5%| ?
-
|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009 08/05]
+
|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|width=5%| A3 <span/ id="A3">
|width=5%| A3 <span/ id="A3">
-
|width=10%| ?
+
|width=10%| clyA_fusion C term
-
|width=10%| [O31] clyA_RBS_Fw
+
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O31 O31 ]  
-
|width=10%| [O10] clyA_fusion_polyG_Rw
+
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O10 O10 ]
-
|width=5%| ?
+
|width=5%| ?(55.5)
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
-
|width=10%| 987bp
+
|width=10%| 987
|width=25%| Amplification of clyA with RBS in the primer
|width=25%| Amplification of clyA with RBS in the primer
|width=5%| ?
|width=5%| ?
-
|width=5%| ? ng/µL
+
|width=5%| ?
-
|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009 08/05]
+
|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|width=5%| A4 <span/ id="A4">
|width=5%| A4 <span/ id="A4">
-
|width=10%| ?
+
|width=10%| OmpAsignal_fusion C term
-
|width=10%| [O11] ompA_signal_Fw
+
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O11 O11 ]  
-
|width=10%| [O12] ompA_signal_fusion_Rw
+
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O12 O12 ]
-
|width=5%| ?
+
|width=5%| ?(70.4)
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
-
|width=10%| 135bp
+
|width=10%| 135
|width=25%| Amplification of OmpA signal for export to periplasm
|width=25%| Amplification of OmpA signal for export to periplasm
|width=5%| ?
|width=5%| ?
-
|width=5%| ? ng/µL
+
|width=5%| ?
-
|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009 08/05]
+
|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|width=5%| A5 <span/ id="A5">
|width=5%| A5 <span/ id="A5">
-
|width=10%| ?
+
|width=10%| tolRII_fusion N term
-
|width=10%| [O1] Rev P1
+
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O13 O13 ]  
-
|width=10%| [O13] tolR_II_Fusion_Fw
+
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O1 O1 ]
-
|width=5%| ?
+
|width=5%| (73.5)
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
-
|width=10%| 279bp
+
|width=10%| 279
|width=25%| Amplification of tolR
|width=25%| Amplification of tolR
|width=5%| ?
|width=5%| ?
-
|width=5%| ? ng/µL
+
|width=5%| ?
-
|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009 08/05]
+
|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|width=5%| A6 <span/ id="A6">
|width=5%| A6 <span/ id="A6">
-
|width=10%| ?
+
|width=10%| tatABC_RBS
-
|width=10%| [O14] tatABC_RBS_Fw
+
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O14 O14 ]  
-
|width=10%| [O5] Rev P5
+
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O15 O15 ]
-
|width=5%| ?
+
|width=5%| (63.5)
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
-
|width=10%| 1634bp
+
|width=10%| 1634
|width=25%| Amplification of tatA with RBS
|width=25%| Amplification of tatA with RBS
|width=5%| ?
|width=5%| ?
-
|width=5%| ? ng/µL
+
|width=5%| ?
-
|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009 08/05]
+
|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
-
|width=5%| A?
+
|width=5%| A7 <span/ id="A7">
|width=10%| g3p_std
|width=10%| g3p_std
-
|width=10%| [O19] g3p_F
+
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O19 O19 ]  
-
|width=10%| [O20] g3p_R
+
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O20 O20 ]
-
|width=5%| 78°C
+
|width=5%| (52)
|width=10%| P12
|width=10%| P12
-
|width=10%| 1287bp
+
|width=10%| 1287
|width=25%| For standardisation of g3p and adding SacI site
|width=25%| For standardisation of g3p and adding SacI site
|width=5%| ?
|width=5%| ?
-
|width=5%| ? ng/µL
 
|width=5%| ?
|width=5%| ?
 +
|width=5%| [https://2009.igem.org/Team:Paris/8_August_2009#PCR 08/08]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
-
|width=5%| A?
+
|width=5%| A8 <span/ id="A8">
|width=10%| OmpA_L_std
|width=10%| OmpA_L_std
-
|width=10%| [O21] Ompa_linker_F
+
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O21 O21 ]  
-
|width=10%| [O22] Ompa_linker_R
+
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O22 O22 ]
-
|width=5%| 80°C
+
|width=5%| (59.5)
|width=10%| P6
|width=10%| P6
-
|width=10%| 525bp
+
|width=10%| 525
|width=25%| For standardisation of OmpA linker (BBaK103006)  
|width=25%| For standardisation of OmpA linker (BBaK103006)  
|width=5%| ?
|width=5%| ?
-
|width=5%| ? ng/µL
 
|width=5%| ?
|width=5%| ?
 +
|width=5%| ?
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| A9 <span/ id="A9">
 +
|width=10%| PFec A
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O29 O29 ] 
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O30 O30 ]
 +
|width=5%| (65.1)
 +
|width=10%| ?
 +
|width=10%| 163
 +
|width=25%| For signal transduction
 +
|width=5%| ?
 +
|width=5%| ?
 +
|width=5%| [https://2009.igem.org/Team:Paris/7_August_2009#PCR 08/07]
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| A10 <span/ id="A10">
 +
|width=10%| clyA_fusion C term
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O31 O31 ] 
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O10 O10 ]
 +
|width=5%| ?(55.5)
 +
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
 +
|width=10%| 987
 +
|width=25%| Amplification of clyA with RBS in the primer
 +
|width=5%| ?
 +
|width=5%| ?
 +
|width=5%| ?
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| A11 <span/ id="A11">
 +
|width=10%| OmpAsignal_fusion C term
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O11 O11 ] 
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O12 O12 ]
 +
|width=5%| ?(70.4)
 +
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
 +
|width=10%| 135
 +
|width=25%| Amplification of OmpA signal for export to periplasm
 +
|width=5%| ?
 +
|width=5%| ?
 +
|width=5%| ?
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| A12 <span/ id="A12">
 +
|width=10%| Tg3p_fusion N term
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O18 O18 ] 
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O17 O17 ]
 +
|width=5%| ? (63.5)
 +
|width=10%| ?
 +
|width=10%| 274
 +
|width=25%| Amplification of N-term domain of M13's g3p
 +
|width=5%| ?
 +
|width=5%| ?
 +
|width=5%| [https://2009.igem.org/Team:Paris/7_August_2009#PCR 08/07]
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| A13 <span/ id="A13">
 +
|width=10%| tolRII_fusion N term
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O13 O13 ] 
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O1 O1 ]
 +
|width=5%| (73.5)
 +
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
 +
|width=10%| 279
 +
|width=25%| Amplification of tolR
 +
|width=5%| ?
 +
|width=5%| ?
 +
|width=5%| ?
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| A14 <span/ id="A14">
 +
|width=10%| tatABC_RBS
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O14 O14 ] 
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O15 O15 ]
 +
|width=5%| (63.5)
 +
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
 +
|width=10%| 1634
 +
|width=25%| Amplification of tatA with RBS
 +
|width=5%| ?
 +
|width=5%| ?
 +
|width=5%| [https://2009.igem.org/Team:Paris/7_August_2009#PCR 08/07]
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| A15 <span/ id="A14">
 +
|width=10%| pFecA
 +
|width=10%| ? 
 +
|width=10%| ?
 +
|width=5%| ?
 +
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
 +
|width=10%| ?
 +
|width=25%| ?
 +
|width=5%| ?
 +
|width=5%| ?
 +
|width=5%| [https://2009.igem.org/Team:Paris/10_August_2009#PCR 08/10]
|}
|}
<br>
<br>
<br>
<br>
-
http://parts.mit.edu/r/parts/images/partbypart/icon_terminator.png
+
http://partsregistry.org/cgi/parts/images/partbypart/icon_terminator.png
-
http://parts.mit.edu/images/partbypart/icon_regulatory.png
+
http://partsregistry.org/images/partbypart/icon_regulatory.png
-
http://parts.mit.edu/r/parts/images/partbypart/icon_rbs.png
+
http://partsregistry.org/cgi/parts/images/partbypart/icon_rbs.png
-
http://parts.mit.edu/r/parts/images/partbypart/icon_reporter.png
+
http://partsregistry.org/cgi/parts/images/partbypart/icon_reporter.png
-
http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png
+
http://partsregistry.org/registry/images/7/7b/Part_icon_cds.png
-
http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png
+
http://partsregistry.org/registry/images/9/9f/Part_icon_dna.png
-
http://parts.mit.edu/registry/images/2/2e/Part_icon_generator.png
+
http://partsregistry.org/registry/images/2/2e/Part_icon_generator.png
-
http://parts.mit.edu/registry/images/a/ab/Part_icon_plasmid.png
+
http://partsregistry.org/registry/images/a/ab/Part_icon_plasmid.png

Latest revision as of 15:28, 19 October 2009

iGEM > Paris > Freezer > PCR Products


PCR products


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol



PCR products
Ref Name Oligo Fw Oligo Rev Tm used(Tm cal.) (°C) Matrix Length (bp) Comments Quantity [ ] (ng/µL) Date
A1 Tg3p_fusion N term O18 O17  ? (63.5)  ? 274 Amplification of N-term domain of M13's g3p  ?  ? 08/05
A2 TE3_fusion N term O15 O16  ?  ??? 1036 Amplification of N-term domain of colicin E3  ?  ? 08/05
A3 clyA_fusion C term O31 O10  ?(55.5) F4 987 Amplification of clyA with RBS in the primer  ?  ? 08/05
A4 OmpAsignal_fusion C term O11 O12  ?(70.4) F4 135 Amplification of OmpA signal for export to periplasm  ?  ? 08/05
A5 tolRII_fusion N term O13 O1 (73.5) F4 279 Amplification of tolR  ?  ? 08/05
A6 tatABC_RBS O14 O15 (63.5) F4 1634 Amplification of tatA with RBS  ?  ? 08/05
A7 g3p_std O19 O20 (52) P12 1287 For standardisation of g3p and adding SacI site  ?  ? 08/08
A8 OmpA_L_std O21 O22 (59.5) P6 525 For standardisation of OmpA linker (BBaK103006)  ?  ?  ?
A9 PFec A O29 O30 (65.1)  ? 163 For signal transduction  ?  ? 08/07
A10 clyA_fusion C term O31 O10  ?(55.5) F4 987 Amplification of clyA with RBS in the primer  ?  ?  ?
A11 OmpAsignal_fusion C term O11 O12  ?(70.4) F4 135 Amplification of OmpA signal for export to periplasm  ?  ?  ?
A12 Tg3p_fusion N term O18 O17  ? (63.5)  ? 274 Amplification of N-term domain of M13's g3p  ?  ? 08/07
A13 tolRII_fusion N term O13 O1 (73.5) F4 279 Amplification of tolR  ?  ?  ?
A14 tatABC_RBS O14 O15 (63.5) F4 1634 Amplification of tatA with RBS  ?  ? 08/07
A15 pFecA  ?  ?  ? F4  ?  ?  ?  ? 08/10



http://partsregistry.org/cgi/parts/images/partbypart/icon_terminator.png http://partsregistry.org/images/partbypart/icon_regulatory.png http://partsregistry.org/cgi/parts/images/partbypart/icon_rbs.png http://partsregistry.org/cgi/parts/images/partbypart/icon_reporter.png http://partsregistry.org/registry/images/7/7b/Part_icon_cds.png http://partsregistry.org/registry/images/9/9f/Part_icon_dna.png http://partsregistry.org/registry/images/2/2e/Part_icon_generator.png http://partsregistry.org/registry/images/a/ab/Part_icon_plasmid.png

Retrieved from "http://2009.igem.org/Team:Paris/Freezer_PCR_products"