Team:Paris/Protocols

From 2009.igem.org

(Difference between revisions)
(→1. Microscopy)
(→2. Adapted Protocols)
 
(86 intermediate revisions not shown)
Line 1: Line 1:
 +
<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Protocols#top | Protocols]] > [[Team:Paris/Protocols#bottom | Main]]
{{Template:Paris2009}}
{{Template:Paris2009}}
{{Template:Paris2009_menu}}
{{Template:Paris2009_menu}}
-
== Protocols ==
 
-
Here you will find the collection of protocole we use, or just collect. And because we are well-known chauvinist it is just a tribute to previous Paris iGEM team .
 
-
We try our best not to make it redondant.
 
-
<center> [[team:Paris/Protocols#Protocols|Main]] - [[Team:Paris/ProtocolsA#Protocols | Microscopy Protocol]] - [[Team:Paris/ProtocolsB#Protocols | Adapted Protocols]] - [[Team:Paris/Protocols_Culture#Protocols | Culture protocols]] - [[Team:Paris/ProtocolsMB#Protocols | Molecular Biology]]</center>
+
==Main==
 +
<html>
 +
<style type="text/css">
 +
#left-side {
 +
    position: absolute;
 +
    height: 23px;
 +
    width: 30px;
 +
    top: 0px;
 +
    left: 40px;
 +
    margin-top:10px;
 +
    padding-top: 7px;
 +
    background: url(https://static.igem.org/mediawiki/2009/1/1b/Left_menu_pari.png);
 +
    z-index:4;
 +
}
 +
 
 +
#middle-side {
 +
    height: 25px;
 +
    width: 560px;
 +
    position: absolute;
 +
    top: 0px;
 +
    left: 60px;
 +
    margin-top:10px;
 +
    padding-top: 5px;
 +
    background: #dadada;
 +
    z-index:5;
 +
}
 +
 
 +
#right-side {
 +
    position: absolute;
 +
    height: 23px;
 +
    width: 30px;
 +
    margin-top:10px;
 +
    padding-top: 7px;
 +
    top: 0px;
 +
    left: 600px;
 +
    background: url(https://static.igem.org/mediawiki/2009/4/40/Right_menu_paris.png);
 +
    z-index:4;
 +
}
-
<html>
+
a.menu_sub {
 +
    padding-left: 7px;
 +
    padding-right: 7px;
 +
}
 +
 
 +
a.menu_sub_active {
 +
    padding-left: 7px;
 +
    padding-right: 7px;
 +
    color:#b0310e;
 +
    font-weight:bold;
 +
}
 +
</style>
 +
<div id="left-side"></div>
 +
<div id="middle-side"><center>
 +
<a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/Protocols#bottom"> Main </a>|
 +
<a class="menu_sub" href="https://2009.igem.org/Team:Paris/ProtocolsA#bottom"> Microscope Protocols</a>|
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/ProtocolsB#bottom"> Adapted Protocols</a>|
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Protocols_Culture#bottom"> Culture Protocols</a>|
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/ProtocolsMB#bottom"> Molecular biology</a>
 +
</center>
</div>
</div>
-
<div id="paris_content_boxtop">
+
 
-
</div>
+
<div id="right-side"></div>
-
<div id="paris_content">
+
 
</html>
</html>
-
==Summary==
+
 
===1. Microscopy===
===1. Microscopy===
* [[Team:Paris/ProtocolsA#Protocols suggested | Protocols suggested]]
* [[Team:Paris/ProtocolsA#Protocols suggested | Protocols suggested]]
 +
*[http://probes.invitrogen.com/media/pis/mp00282.pdf Protocol to membrane dying, DID method (pdf)]
===2. Adapted Protocols===
===2. Adapted Protocols===
* DNA extraction
* DNA extraction
-
* Mini prep
+
* [[Team:Paris/ProtocolsB#Adaptation of PureYield&trade; Plasmid Miniprep System (Promega) |Mini prep ]]
-
* Gel/PCR Purification
+
* Gel/PCR Purification
 +
 
===3. Culture protocols===
===3. Culture protocols===
* Over Night
* Over Night
-
* Transformation
+
*[[Team:Paris/Protocols_Culture#Bacterial transformation | Bacterial transformation]]
-
* [[Team:Paris/Protocols_Culture#Competent bacterias | Competent bacterias]]
+
* [[Team:Paris/Protocols_Culture#Competent bacteria | Competent bacteria]]
**[[team:Paris/Protocols_Culture#CaCl2a|CaCl<sub>2</sub> (iGEM Paris2007)]]  
**[[team:Paris/Protocols_Culture#CaCl2a|CaCl<sub>2</sub> (iGEM Paris2007)]]  
**[[team:Paris/Protocols_Culture#CaCl2b|CaCl<sub>2</sub> (2nd Protocol)]]  
**[[team:Paris/Protocols_Culture#CaCl2b|CaCl<sub>2</sub> (2nd Protocol)]]  
**[[team:Paris/Protocols_Culture#RbCl|RbCl]]  
**[[team:Paris/Protocols_Culture#RbCl|RbCl]]  
* [[team:Paris/Protocols_Culture#Glycerol stock|Glycerol stock]]
* [[team:Paris/Protocols_Culture#Glycerol stock|Glycerol stock]]
 +
*[[team:Paris/Protocols_Culture#Ferric citrate growth medium|Ferric citrate growth medium]]
 +
===4. Molecular biology===
===4. Molecular biology===
-
* PCR
+
*[[Team:Paris/ProtocolsMB#PCR with Quick load Taq2x Master Mix|PCR]]
 +
*[[Team:Paris/ProtocolsMB#PCR colonies|PCR colony]]
* Gels
* Gels
* Digestion
* Digestion
* Ligation
* Ligation
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
-----
 
-
-----
 
-
-----
 
-
-----
 
-
-----
 
-
 
-
*[[Team:Paris/Protocols_Competent_Bacteria | Protocol to make competent bacteria]]
 
-
*[[Team:Paris/Protocols_PCRqload | Protocole PCR quick load Taq mix]]
 
-
*[http://probes.invitrogen.com/media/pis/mp00282.pdf Protocol to membrane dying, DID method]
 
-
*[[Team:Paris/Miniprep | Adaptation of PureYield&trade; Plasmid Miniprep System]]
 
-
*[[Team:Paris/Protocols_transformation | Protocol for bacterial transformation]]
 
-
 
-
====A.2. iGEM paris 2008 protocols link====
 
-
 
-
[https://2008.igem.org/Team:Paris/Notebook/Protocols Click here]
 
-
 
-
 
-
<u>content :</u>
 
-
 
-
*Electrophoresis
 
-
*Concentration of the Miniprep or the Midiprep
 
-
*Amplification of promoters.
 
-
*PCR Screening
 
-
*Protocol to make competent bacteria
 
-
 
-
 
-
====A.3. iGEM paris 2007 protocols link====
 
-
 
-
[http://parts.mit.edu/igem07/index.php/Paris/PROTOCOLS Click here]
 
-
 
-
 
-
<u>content :</u>
 
-
 
-
*Growing_bacteria_in_liquid_medium
 
-
*Preparing growth media
 
-
**exemple: Making 10 petri dish (LB+erythromycin+citrate+DAP), Solid M9 Minimum Medium, preparation of agarosis gel
 
-
*Chemical transformation
 
-
*Glycerol Stock
 
-
*Recombineering/Lambda red-mediated gene replacement
 
-
*Miniprep
 
-
*Fluorescent single cells visualisation
 
-
*Digestion reactions
 

Latest revision as of 16:35, 20 October 2009

iGEM > Paris > Protocols > Main


Contents

Main

1. Microscopy

  • Protocols suggested
  • [http://probes.invitrogen.com/media/pis/mp00282.pdf Protocol to membrane dying, DID method (pdf)]

2. Adapted Protocols

3. Culture protocols

4. Molecular biology

Retrieved from "http://2009.igem.org/Team:Paris/Protocols"