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Team:Virginia/1 June 2009
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ThaddeusWebb (Talk | contribs) (New page: Used the total genome extract from friday to do PCR run with ArsC primers. *used low concentration of MgCl .7 mM.) |
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| - | Used the total genome extract from | + | {{Template:VirginiaNotebook}} |
| - | * | + | |
| + | ==Overnight Results== | ||
| + | *All plates had colonies | ||
| + | **Including negative control | ||
| + | **BFP + B0032 assembly had few colonies | ||
| + | |||
| + | ==Today== | ||
| + | *Ran PCR of E.coli total genomic isolate with primers for ArsC | ||
| + | **Used the total genome extract from Friday to do PCR run with ArsC primers. | ||
| + | |||
| + | *Extracted plasmid DNA from MED1, PSB1K3, BFP + B0032, and PSB1A3.* | ||
| + | *Ran spectrophotometer on extracted plasmid DNA.* | ||
| + | |||
| + | *Digested MED1 and BFP + B0032 with X and P.* | ||
| + | --[[User:Bsf2u|Bsf2u]] 20:45, 1 June 2009 (UTC) | ||
Latest revision as of 20:45, 1 June 2009
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Overnight Results
- All plates had colonies
- Including negative control
- BFP + B0032 assembly had few colonies
Today
- Ran PCR of E.coli total genomic isolate with primers for ArsC
- Used the total genome extract from Friday to do PCR run with ArsC primers.
- Extracted plasmid DNA from MED1, PSB1K3, BFP + B0032, and PSB1A3.*
- Ran spectrophotometer on extracted plasmid DNA.*
- Digested MED1 and BFP + B0032 with X and P.*
--Bsf2u 20:45, 1 June 2009 (UTC)
