Team:UNICAMP-Brazil/Notebooks/August 6

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== YeastGuard - Selection of Biobricks ==
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{{:Team:UNICAMP-Brazil/inc_topo}}
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{{:Team:UNICAMP-Brazil/inc calendar}}
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- Today we chose 7 biobricks for the yeastguard project. We recovered and resuspended it.
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__NOTOC__
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- Transformed the biobricks into ''E. coli'' DH10B competent cells and plated on Kan or Amp plates, according to information from registry page.
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==''' YeastGuard '''==
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- Prayed to find colonies tomorrow. =)
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====Recovering the Biobricks====
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* Today we resuspended the 7 biobricks of the Yeastguard subproject.
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- Chosen Biobricks: BBa_J63005, BBa_J63003, BBa_J63002, BBa_E2030, BBa_E2020, BBa_J04630 and BBa_J63010.
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* We transformed the biobricks into ''E. coli'' DH10B competent cells and plated on Kan or Amp plates, according to information from registry page.
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* Prayed to find colonies tomorrow. =)
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''Raíssa and Wesley''
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====RNA Extraction====
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* Today we performed RNA extration from yeast ''Kluyveromyces lactis'' according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Yeast_RNA_Extraction Protocol 6].  
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''Wesley''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Latest revision as of 00:10, 22 October 2009

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YeastGuard

Recovering the Biobricks

  • Today we resuspended the 7 biobricks of the Yeastguard subproject.
  • We transformed the biobricks into E. coli DH10B competent cells and plated on Kan or Amp plates, according to information from registry page.
  • Prayed to find colonies tomorrow. =)

Raíssa and Wesley

RNA Extraction

  • Today we performed RNA extration from yeast Kluyveromyces lactis according to Protocol 6.

Wesley




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