|
|
| (40 intermediate revisions not shown) |
| Line 1: |
Line 1: |
| - | ==To find==
| |
| - | PDB files of LOV domain, TrpR repressor => http://www.rcsb.org/pdb/home/home.do
| |
| | | | |
| - | ==To do==
| |
| - | <br> <font size=3><span style="color:red"> OK </span></font size>- Set up day of starting modeling, do a planning for modeling => see with Matteo Dal Peraro <span style="color:red"><b> ----> will be setted up as soon as the exams are over </b></span>
| |
| - | <br>- Model allosteric interactions between LOVTAP & TrpR:
| |
| - | What will be done:
| |
| - | :-> model of LOVTAP in dark phase
| |
| - | :-> model of LOVTAP in light phase
| |
| - | :-> characterize how the J-alpha helix changes
| |
| - |
| |
| - | We will follow the following article protocol:
| |
| - | <br> Freddolino, P.L., Dittrich M., Schulten K., Dynamic Switching Mechanisms in LOV1 and LOV2 Domains of Plant Phototropins. Biophysical Journal, <b> 91 </b>, 3630-3639, 2006 (http://www.ncbi.nlm.nih.gov/ => PMC1630464)
| |
| - |
| |
| - | <br>- Model the fusion of LOV domain with other transcription factors
| |
| - |
| |
| - | <br>- Maybe do a mutational assay to change specificity of LOVTAP:
| |
| - | :Direct mutational assay: Insert mutation in specific sites thanks to the known structure of LOVTAP (already modeled on computer)
| |
| - | :Indirect mutational assay: Random mutations, then selection of the "right" protein according to a set of selected conditions
| |
| - |
| |
| - | ==To envisage ==
| |
| - | <br>- Molecular mutationnal assay
| |
| - |
| |
| - | ----
| |
| - | {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
| |
| - | !align="center"|[[Team:EPF-Lausanne|Home]]
| |
| - | !align="center"|[[Team:EPF-Lausanne/Team|The Team]]
| |
| - | !align="center"|[[Team:EPF-Lausanne/Project|The Project]]
| |
| - | !align="center"|[[Team:EPF-Lausanne/Parts|Parts Submitted to the Registry]]
| |
| - | !align="center"|[[Team:EPF-Lausanne/Modeling|Modeling]]
| |
| - | !align="center"|[[Team:EPF-Lausanne/Notebook|Notebook]]
| |
| - | !align="center"|[[Team:EPF-Lausanne/Lectures|Lectures]]
| |
| - | !align="center"|[[Team:EPF-Lausanne/Team Management|Team Management]]
| |
| - | |}
| |
"
