Team:Groningen/Parts

From 2009.igem.org

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{{Team:Groningen/Header}}
{{Team:Groningen/Header}}
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<!--- The Mission, Experiments --->
 
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===Note===
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<div style="float:left" >{{linkedImage|GroningenPrevious.png|Team:Groningen/Ethics/Survey}}</div>
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<div title="Arsie Says UP TO ACCUMULATION" style="float:right">{{linkedImage|Next.JPG|Team:Groningen/Parts/Submitted_Parts}}</div>
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If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here.  This is not necessary but it may be a nice list to keep track of.
 
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:::::::::::::::http://partsregistry.org/wiki/images/d/db/Buttonaddpart.gif
 
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<b><h2>[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen Our sandbox]</h2></b>
 
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*For a comprehensive list of all the parts we used, have a look at our [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen '''parts registry'''].
 +
*Here you can find an informative overview of our [[Team:Groningen/Modelling/Submitted Parts|'''submitted parts''']].
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*Experience we had with parts from the registry can be found under [[Team:Groningen/Modelling/Used Parts|'''used parts''']].
 +
*Need anything specific? Take a look in our [[Team:Groningen/Parts/Freezer|freezer]]
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===Part List -80===
 
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{|cellpadding="10" cellspacing="1" border="10" style="font-size: large;"
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|'''Component'''
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{{Team:Groningen/Footer}}
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|'''Description'''
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|'''Part or Accession #'''
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|'''Base Pairs (bp)'''
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|'''Plasmid (backbone)'''
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|'''Resistance'''
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|'''Well'''
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|'''Quality control'''
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|-
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|GVP
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|Gas Vesicle Proteins
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|[http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
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|6064
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|[http://partsregistry.org/Part:BBa_J61035 BBa_J61035]
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|Ampicillin/Gentamycin
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|plate 3, pos.1
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|?
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|-
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|GVP (2nd batch)
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|Gas Vesicle Proteins
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|[http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
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|6064
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|[http://partsregistry.org/Part:BBa_J61035 BBa_J61035]
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|Ampicillin/Gentamycin
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|plate 3, pos.2
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|?
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|-
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|pHigh in low copy nr vector
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|High consitutive promoter
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|<partinfo>J23100</partinfo>
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|35bp
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|<partinfo>pSB3K3</partinfo>
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|Kanamycin
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|Plate 3, pos. 4
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|PCR(?), restriction(+)
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|-
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|pMed in low copy nr vector
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|Medium consitutive promoter
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|<partinfo>J23106</partinfo>
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|35bp
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|<partinfo>pSB3K3</partinfo>
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|Kanamycin
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|Plate 3, pos. 5
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|PCR(?), restriction(+)
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|-
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|pLow in low copy nr vector
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|Low consitutive promoter
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|<partinfo>J23109</partinfo>
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|35bp
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|<partinfo>pSB3K3</partinfo>
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|Kanamycin
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|Plate 3, pos. 6
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|PCR(?), restriction(+)
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|-
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|pHigh in high copy nr vector
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|High consitutive promoter
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|<partinfo>J23100</partinfo>
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|35bp
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|<partinfo>pSB1AC3</partinfo>
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|Ampicillin/Chloramphenicol
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|Plate 3, pos. 7
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|PCR(?), restriction(+)
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|-
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|pMed in high copy nr vector
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|Medium consitutive promoter
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|<partinfo>J23106</partinfo>
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|35bp
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|<partinfo>pSB1AC3</partinfo>
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|Ampicillin/Chloramphenicol
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|Plate 3, pos. 8
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|PCR(?), restriction(+)
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|-
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|pLow in high copy nr vector
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|High consitutive promoter
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|<partinfo>J23109</partinfo>
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|35bp
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|<partinfo>pSB1AC3</partinfo>
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|Ampicillin/Chloramphenicol
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|Plate 3, pos. 9
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|PCR(?), restriction(+)
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|-
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|Promotor (low expression)
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|Constitutive promotor
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|[http://partsregistry.org/Part:BBa_J23109 BBa_J23109]
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|35
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|[http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
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|Ampicillin
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|plate 3, pos.10
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|No
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|-
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|Promotor (medium expression)
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|Constitutive promotor
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|[http://partsregistry.org/Part:BBa_J23106 BBa_J23106]
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|35
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|[http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
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|Ampicillin
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|plate 3, pos.11
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|No
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|-
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|Promotor (high expression)
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|Constitutive promotor
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|[http://partsregistry.org/Part:BBa_J23100 BBa_J23100]
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|35
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|[http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
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|Ampicillin
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|plate 3, pos.12
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|No
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|-
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|Inducible promoter
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|Lactose inducible promoter nr1
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|<partinfo>R0010</partinfo>
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|200bp
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|<partinfo>pSB1A2</partinfo>
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|Ampicillin
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|Plate 3, pos. 14
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|PCR(?), restriction(+)
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|-
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|Inducible promoter
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|Lactose inducible promoter nr2
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|<partinfo>R0010</partinfo>
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|200bp
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|<partinfo>pSB1A2</partinfo>
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|Ampicillin
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|Plate 3, pos. 15
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|PCR(?), restriction(+)
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|-
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|RBS
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|Ribosomal Binding Site
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|[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]
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|12
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|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
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|Ampicillin
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|plate 3, pos.19
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|No
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|-
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|Terminator
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|Double terminator consisting of [http://partsregistry.org/Part:BBa_B0012 BBa_B0012] and [http://partsregistry.org/Part:BBa_B0011 BBa_B0011]
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|[http://partsregistry.org/Part:BBa_B0014 BBa_B0014]
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|~90
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|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
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|Ampicillin/Kanamycin
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|plate 3, pos.20
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|No
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|-
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|Const. Pormoter + GVP
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|Low constitutive promoter with GVP cluster
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|[http://partsregistry.org/Part:BBa_J23109 BBa_J23109] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
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|~6100
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|[http://partsregistry.org/Part:pSB1AC3 pSB1AC3]
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|Ampicillin/Chloramphenicol
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|plate 3, pos.21
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|EcoRI/PstI
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|-
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|Const. Pormoter + GVP (no.2)
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|Low constitutive promoter with GVP cluster
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|[http://partsregistry.org/Part:BBa_J23109 BBa_J23109] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
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|~6100
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|[http://partsregistry.org/Part:pSB1AC3 pSB1AC3]
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|Ampicillin/Chloramphenicol
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|plate 3, pos.22
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|EcoRI/PstI
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|-
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|Const. Pormoter + GVP
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|Medium constitutive promoter with GVP cluster
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|[http://partsregistry.org/Part:BBa_J23106 BBa_J23106] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
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|~6100
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|[http://partsregistry.org/Part:pSB1AC3 pSB1AC3]
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|Ampicillin/Chloramphenicol
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|plate 3, pos.23
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|EcoRI/PstI
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|-
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|Const. Pormoter + GVP (no.2)
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|Medium constitutive promoter with GVP cluster
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|[http://partsregistry.org/Part:BBa_J23106 BBa_J23106] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
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|~6100
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|[http://partsregistry.org/Part:pSB1AC3 pSB1AC3]
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|Ampicillin/Chloramphenicol
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|plate 3, pos.24
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|EcoRI/PstI
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|-
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|HmtA (1.1)
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|HmtA-His in pBAD(a)
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|None
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|?
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|None
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|Ampicillin
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|plate 3, pos.28
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|Restriction(+)
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|-
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|HmtA (2.1)
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|HmtA-His in pBAD(a)
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|None
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|?
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|None
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|Ampicillin
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|plate 3, pos.29
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|Restriction(+)
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|-
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|CueO- + GVP in J61035
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|CueO promoter without RBS in front of the GVP cluster
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|None
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|6149
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI)
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|Ampicillin
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|plate 3, pos.37
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|Restriction(+)
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|-
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|ZntR- + GVP in J61035
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|ZntR promoter without RBS in front of the GVP cluster
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|None
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|~6150
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI)
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|Ampicillin
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|plate 3, pos.38
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|Restriction(+)
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|-
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|ArsR- + GVP in J61035
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|ArsR promoter without RBS in front of the GVP cluster
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|None
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|~6150
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI)
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|Ampicillin
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|plate 3, pos.39
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|Restriction(+)
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-
|}
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Latest revision as of 09:41, 22 January 2010

[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
Biobricks


[http://2009.igem.org/Team:Groningen/Ethics/Survey http://2009.igem.org/wiki/images/1/1f/GroningenPrevious.png]
[http://2009.igem.org/Team:Groningen/Parts/Submitted_Parts http://2009.igem.org/wiki/images/d/dd/Next.JPG]



  • For a comprehensive list of all the parts we used, have a look at our [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen parts registry].
  • Here you can find an informative overview of our submitted parts.
  • Experience we had with parts from the registry can be found under used parts.
  • Need anything specific? Take a look in our freezer