Template:Team:KULeuven/13 August 2009/VanillinProduction
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- | # Plated | + | # Plated ''COMT'' (+RBS) from the registry |
- | #* Growing overnight in | + | #* Growing overnight in 37°C |
- | # Plated | + | # Plated ''ech'' |
#* From newly made liquid culture | #* From newly made liquid culture | ||
- | # Miniprep of | + | # Miniprep of ''ech'' and ''sam8'' |
#* Nanodrop results: | #* Nanodrop results: | ||
{| border ="1" align="center" | {| border ="1" align="center" | ||
!| Part || concentration (ng/μl) || 260/280 λ || 260/230 λ | !| Part || concentration (ng/μl) || 260/280 λ || 260/230 λ | ||
|- align="center" | |- align="center" | ||
- | | | + | | ''ech'' ||72,7 || 1,94 || |
|- align="center" | |- align="center" | ||
- | | | + | | ''sam8'' ||66,2|| 2,00 || |
|- | |- | ||
|} | |} | ||
- | # Restriction digest on | + | # Restriction digest on ''ech'' and ''sam8'' |
- | # | + | # Gel electrophoresis on ''ech'' and ''sam8'' |
- | # New electrocompetent cells were used for the electroporation | + | # New electrocompetent cells were used for the electroporation with the old ligations |
- | #* Lig A -> | + | #* Lig A -> ''sam5 + sam8'' |
- | #* Lig C -> | + | #* Lig C -> ''ech + fcs'' |
#* Note: there was no spark | #* Note: there was no spark | ||
# Plating of the electroporated cells | # Plating of the electroporated cells | ||
#* 200 μl per plate twice | #* 200 μl per plate twice | ||
#* 600 μl left of A and C | #* 600 μl left of A and C |
Latest revision as of 10:01, 14 September 2009
- Plated COMT (+RBS) from the registry
- Growing overnight in 37°C
- Plated ech
- From newly made liquid culture
- Miniprep of ech and sam8
- Nanodrop results:
Part | concentration (ng/μl) | 260/280 λ | 260/230 λ |
---|---|---|---|
ech | 72,7 | 1,94 | |
sam8 | 66,2 | 2,00 |
- Restriction digest on ech and sam8
- Gel electrophoresis on ech and sam8
- New electrocompetent cells were used for the electroporation with the old ligations
- Lig A -> sam5 + sam8
- Lig C -> ech + fcs
- Note: there was no spark
- Plating of the electroporated cells
- 200 μl per plate twice
- 600 μl left of A and C