Template:Team:KULeuven/14 August 2009/VanillinReceptor

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(Difference between revisions)
(New page: # VirG 2,3,1' on gel electrophorese #* 2 and 3 look oke, 1000bp #* 1' looks oke #* 800bp fragment + 200bp TOPO) # Innoculate 2 and 3 and grow on.... # TOPO from α and s and again fro...)
 
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# VirG 2,3,1' on gel electrophorese
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# G 2,3,1' on gel electrophoresis
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#* 2 and 3 look oke, 1000bp
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#* 2 and 3 seem oke, 1000bp
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#* 1' looks oke
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#* 1' seems oke
#* 800bp fragment + 200bp TOPO)
#* 800bp fragment + 200bp TOPO)
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# Innoculate 2 and 3 and grow on....
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# Inoculate 2 and 3 and grow on ....
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# TOPO from α and s and again from VirA
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# TOPO from W and Z and again from A
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#* Note: if VirA does not succes again (with the iGEM protocol) then proceed with a new PCR purification.
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#* Note: if A fails again (with the iGEM protocol) then proceed with a new PCR purification.
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# Mutagenesis 1 on position 202 of R was performed

Latest revision as of 09:03, 8 September 2009

  1. G 2,3,1' on gel electrophoresis
    • 2 and 3 seem oke, 1000bp
    • 1' seems oke
    • 800bp fragment + 200bp TOPO)
  2. Inoculate 2 and 3 and grow on ....
  3. TOPO from W and Z and again from A
    • Note: if A fails again (with the iGEM protocol) then proceed with a new PCR purification.
  4. Mutagenesis 1 on position 202 of R was performed
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