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Revision as of 07:57, 16 August 2009 (view source) Unocchi (Talk | contribs) (New page: =parts planning= '''signal sensor<br>''' 1.3O6HSL<br> Image:Planning_osaka.PNG<br> P(tetR)+luxI<br> 2.C4HSL<br> Image:Planning_osaka.PNG<br> P(tetR)+rhlI<br> 3.AI-1<br> [...) ← Older edit		Latest revision as of 07:54, 12 October 2009 (view source) Unocchi (Talk | contribs)
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-	=parts planning=	+	'''parts planning'''
-	'''signal sensor<br>'''	+	teble sample
		+	<table border="1" Frame="box">
		+	<tr><td>Vector</td><td>Insert</td></tr>
		+	<tr><td>name</td><td>name</td></tr>
		+	<tr><td>link to BBa~</td><td>link to BBa~</td><tr>
		+	<tr><td>total size (bp)</td><td>insert size (bp)</td><tr>
		+	<tr><td> Plasmid</td><td> </td></tr>
		+	</table>
		+	<br>
		+	=Signal generator=
		+	'''signal generator<br>'''
		+	[[Image:Planning_osaka.PNG]]<br>
-	1.3O6HSL<br>	+	=='''3O<sub>6</sub>HSL'''  P(tetR)+luxI<br>==
-	[[Image:Planning_osaka.PNG]]<br>	+
-	P(tetR)+luxI<br>	+
		+	1.RBS + luxI<br>
		+	<table border="1" Frame="box">
		+	<tr><td>Vector(S+P)</td><td>Insert(X+P)</td></tr>
		+	<tr><td>RBS</td><td>luxI</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]</td><td>[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]</td><tr>
		+	<tr><td>2091bp</td><td>585bp</td></tr>
		+	<tr><td>pSB1A2</td><td> </td></tr>
		+	</table>
-	2.C4HSL<br>
-	[[Image:Planning_osaka.PNG]]<br>
-	P(tetR)+rhlI<br>
		+	2.RBS+ luxI + terminator<br>
		+	<table border="1" Frame="box">
		+	<tr><td>Vector(E+X)</td><td>Insert(E+S)</td></tr>
		+	<tr><td>double terminator</td><td>RBS+luxI</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]</td><td> </td></tr>
		+	<tr><td>3189bp</td><td>579bp</td></tr>
		+	<tr><td>pSB1AK3</td><td></td></tr>
		+	</table>
-	3.AI-1<br>	+	3.P(tetR)+RBS+ luxI + terminator<br>
-	[[Image:Planning_osaka.PNG]]<br>	+	<table border="1" Frame="box">
-	P(tetR)+lasI<br>	+	<tr><td>Vector(S+P)</td><td>Insert(X+P)</td></tr>
		+	<tr><td>p(tetR)</td><td>RBS+luxI+terminator</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]</td><td> </td></tr>
		+	<tr><td>2133bp</td><td>726bp</td></tr>
		+	<tr><td>pSB1A2</td><td> </td></tr>
		+	</table>
		+	=='''C<sub>4</sub>HSL'''  P(tetR)+rhlI<br>==
		+	1.RBS + rhII
		+	<table border="1" Frame="box">
		+	<tr><td>Vector(S+P)</td><td>Insert(X+P)</td></tr>
		+	<tr><td>RBS</td><td>rhII</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]</td><td>[http://partsregistry.org/Part:BBa_C0170 BBa_C0170]</td><tr>
		+	<tr><td>2091bp</td><td>609bp</td></tr>
		+	<tr><td>pSB1A2</td><td> </td></tr>
		+	</table>
-	4.3OH,C14:1-HSL<br>
-	[[Image:Planning_osaka.PNG]]<br>
-	P(tetR)+cinI<br>
		+	2.RBS + rhII + terminator
		+	<table border="1" Frame="box">
		+	<tr><td>Vector(E+X)</td><td>Insert(E+S)</td></tr>
		+	<tr><td>double terminator</td><td>RBS + rhII </td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]</td><td> </td></tr>
		+	<tr><td>3189bp</td><td>621bp</td></tr>
		+	<tr><td>pSB1AK3</td><td></td></tr>
		+	</table>
		+	3.P(tetR) + RBS + rhlI + terminator<br>
		+	<table border="1" Frame="box">
		+	<tr><td>Vector(S+P)</td><td>Insert(X+P)</td></tr>
		+	<tr><td>p(tetR)</td><td>RBS + rhII +terminator</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]</td><td> </td></tr>
		+	<tr><td>2625bp</td><td>750bp</td></tr>
		+	<tr><td>pSB1A2</td><td></td></tr>
		+	</table>
		+	=='''AI-1'''  P(tetR)+lasI==
		+
		+	1.RBS + LasI
		+	<table border="1" Frame="box">
		+	<tr><td>Vector(S+P)</td><td>Insert(X+P)</td></tr>
		+	<tr><td>RBS</td><td>LasI</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]</td><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0078 BBa_C0078]</td></tr>
		+	<tr><td>2091bp</td><td>609bp</td></tr>
		+	<tr><td>pSB1A2</td><td> </td></tr>
		+	</table>
		+
		+
		+	2.RBS + LasI +terminator
		+	<table border="1" Frame="box">
		+	<tr><td>Vector(E+X)</td><td>Insert(E+S)</td></tr>
		+	<tr><td>double terminator</td><td>RBS + LasI +terminator</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]</td><td> </td></tr>
		+	<tr><td>3189bp</td><td>654bp</td></tr>
		+	<tr><td>pSB1AK3</td><td></td></tr>
		+	</table>
		+
		+
		+	3.P(tetR) + RBS + LasI +terminator
		+	<table border="1" Frame="box">
		+	<tr><td>Vector(S+P)</td><td>Insert(X+P)</td></tr>
		+	<tr><td>p(tetR)</td><td>RBS + LasI +terminator</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]</td><td> </td></tr>
		+	<tr><td>2091bp</td><td>609bp</td></tr>
		+	<tr><td>pSB1A2</td><td></td></tr>
		+	</table>
		+
		+	=='''3OH,C<sub>14</sub>:1-HSL'''  P(tetR)+cinI<br>==
		+
		+	1. RBS + cinI<br>
		+	'''K204006'''
		+	<table border="1" Frame="box">
		+	<tr><td>Vector(S+P)</td><td>Insert(X+P)</td></tr>
		+	<tr><td>RBS</td><td>cinI</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]</td><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 BBa_C0076]</td></tr>
		+	<tr><td>2091bp</td><td>702bp</td></tr>
		+	<tr><td>pSB1A2</td><td> </td></tr>
		+	</table>
		+
		+
		+	2.RBS + cinI +terminator
		+	<table border="1" Frame="box">
		+	<tr><td>Vector(E+X)</td><td>Insert(E+S)</td></tr>
		+	<tr><td>double terminator</td><td>RBS + cinI</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]</td><td> </td></tr>
		+	<tr><td>3189bp</td><td>714bp</td></tr>
		+	<tr><td>pSB1AK3</td><td></td></tr>
		+	</table>
		+
		+
		+	3.P(tetR) + RBS + cinI +terminator
		+	<table border="1" Frame="box">
		+	<tr><td>Vector(S+P)</td><td>Insert(X+P)</td></tr>
		+	<tr><td>p(tetR)</td><td>RBS + cinI +terminator</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]</td><td> </td></tr>
		+	<tr><td>2133bp</td><td>843bp</td></tr>
		+	<tr><td>pSB1A2</td><td></td></tr>
		+	</table>
		+
		+	=receiver=
	'''signal receiver<br>'''		'''signal receiver<br>'''
-	1.3O6HSL<br>	+	[[Image:Planning osaka receiver.PNG]]<br>
-	[[Image:Planning osaka receiver.PNG]]<br>	+
-	luxpL+luxR+luxpR<br>	+
		+	=='''3O<sub>6</sub>HSL'''  luxpL+luxR+luxpR==
		+	[http://partsregistry.org/Part:BBa_F2621 BBa_F2621](1158bp)<br>
		+	pSB1A2<br>
		+
		+
		+	=='''C<sub>4</sub>HSL'''  p(tetR) + rhlR  +  RhlR+C<sub>4</sub>==
		+	1.RhlR+p(Rhl)<br>
		+	'''K204005'''
		+	<table border="1" Frame="box">
		+	<tr><td>Vector(E+X)</td><td>Insert(E+S)</td></tr>
		+	<tr><td>p(RhlR)</td><td>RhlR</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_I14017 BBa_I14017]</td><td>[http://partsregistry.org/Part:BBa_I1466 BBa_I1466]</td></tr>
		+	<tr><td>4476bp</td><td>884bp</td></tr>
		+	<tr><td>pSB2K3</td><td></td></tr>
		+	</table>
		+
		+	2.p(tetR)+RhlR+p(Rhl)
		+	<table border="1" Frame="box">
		+	<tr><td>Vector(S+P)</td><td>Insert(X+P)</td></tr>
		+	<tr><td>p(tetR)</td><td>RhlR+p(Rhl)</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]</td><td> </td></tr>
		+	<tr><td>2133bp</td><td>935bp</td></tr>
		+	<tr><td>pSB1A2</td><td></td></tr>
		+	</table>
		+
		+	=='''AI-1'''  p(tetR) + lasR +  LasR+pAI==
		+	1. RBS + lasR<br>
		+	'''K204001''','''K204004'''
		+	<table border="1" Frame="box">
		+	<tr><td>Vector(S+P)</td><td>Insert(X+P)</td></tr>
		+	<tr><td>RBS</td><td>lasR</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]</td><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0179 BBa_C0179]</td></tr>
		+	<tr><td>2091bp</td><td>723bp</td></tr>
		+	<tr><td>pSB1A2</td><td> </td></tr>
		+	</table>
		+
		+
		+	2.RBS + lasR +terminator
		+	<table border="1" Frame="box">
		+	<tr><td>Vector(E+X)</td><td>Insert(E+S)</td></tr>
		+	<tr><td>double terminator</td><td>RBS + lasR +terminator</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]</td><td> </td></tr>
		+	<tr><td>3189bp</td><td>735bp</td></tr>
		+	<tr><td>pSB1AK3</td><td></td></tr>
		+	</table>
		+
		+
		+	3.P(tetR) + RBS + lasR +terminator
		+	<table border="1" Frame="box">
		+	<tr><td>Vector(S+P)</td><td>Insert(X+P)</td></tr>
		+	<tr><td>p(tetR)</td><td>RBS + lasR +terminator</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]</td><td> </td></tr>
		+	<tr><td>2625bp</td><td>864bp</td></tr>
		+	<tr><td>pSB1A2</td><td></td></tr>
		+	</table>
		+
		+
		+	4.P(tetR) + RBS + lasR +terminator +lasR+pAI
		+	<table border="1" Frame="box">
		+	<tr><td>Vector(E+X)</td><td>Insert(E+S)</td></tr>
		+	<tr><td>lasR+pAI</td><td>P(tetR) + RBS + lasR +terminator</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_R0079 BBa_R0079]</td><td> </td></tr>
		+	<tr><td>2243bp</td><td>918bp</td></tr>
		+	<tr><td>pSB1A2</td><td></td></tr>
		+	</table>
		+	=='''3OH,C<sub>14</sub>:1-HSL'''  p(tetR) + cinR + cinR==
		+	1. RBS + cinR<br>
		+	'''K204003'''
		+	<table border="1" Frame="box">
		+	<tr><td>Vector(S+P)</td><td>Insert(X+P)</td></tr>
		+	<tr><td>RBS</td><td>cinR</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]</td><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 BBa_C0077]</td></tr>
		+	<tr><td>2091bp</td><td>762bp</td></tr>
		+	<tr><td>pSB1A2</td><td> </td></tr>
		+	</table>
-	2.C4HSL<br>	+	2.RBS + cinR +terminator
-	[[Image:Planning osaka receiver.PNG]]<br>	+	<table border="1" Frame="box">
-	p(tetR) + rhlR  +  RhlR+C4<br>	+	<tr><td>Vector(E+X)</td><td>Insert(E+S)</td></tr>
		+	<tr><td>double terminator</td><td>RBS + cinR</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]</td><td> </td></tr>
		+	<tr><td>3189bp</td><td>774bp</td></tr>
		+	<tr><td>pSB1AK3</td><td></td></tr>
		+	</table>
		+	3.P(tetR) + RBS + cinR +terminator
		+	<table border="1" Frame="box">
		+	<tr><td>Vector(S+P)</td><td>Insert(X+P)</td></tr>
		+	<tr><td>p(tetR)</td><td>RBS + cinR +terminator</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]</td><td> </td></tr>
		+	<tr><td>2133bp</td><td>903bp</td></tr>
		+	<tr><td>pSB1A2</td><td></td></tr>
		+	</table>
-	3.AI-1<br>
-	[[Image:Planning osaka receiver.PNG]]<br>
-	p(tetR) + lasR +  LasR+pAI
		+	4.P(tetR) + RBS + cinR +terminator +p(cinR)
		+	<table border="1" Frame="box">
		+	<tr><td>Vector(E+X)</td><td>Insert(E+S)</td></tr>
		+	<tr><td>p(cinR)</td><td>P(tetR) + RBS + cinR +terminator</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_R0078 BBa_R0078]</td><td> </td></tr>
		+	<tr><td>2304bp</td><td>957bp</td></tr>
		+	<tr><td>pSB1A2</td><td></td></tr>
		+	</table>
-	4.3OH,C14:1-HSL<br>	+	[https://2009.igem.org/Team:Osaka/NOTES back to NOTES]
-	[[Image:Planning osaka receiver.PNG]]<br>	+
-	p(tetR) + cinR + cinR	+

---

Latest revision as of 07:54, 12 October 2009

parts planning

teble sample

Vector	Insert
name	name
link to BBa~	link to BBa~
total size (bp)	insert size (bp)
Plasmid

Contents 1 Signal generator 1.1 3O6HSL P(tetR)+luxI 1.2 C4HSL P(tetR)+rhlI 1.3 AI-1 P(tetR)+lasI 1.4 3OH,C14:1-HSL P(tetR)+cinI 2 receiver 2.1 3O6HSL luxpL+luxR+luxpR 2.2 C4HSL p(tetR) + rhlR + RhlR+C4 2.3 AI-1 p(tetR) + lasR + LasR+pAI 2.4 3OH,C14:1-HSL p(tetR) + cinR + cinR

Signal generator

signal generator
 

3O₆HSL P(tetR)+luxI

1.RBS + luxI

Vector(S+P)	Insert(X+P)
RBS	luxI
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]	[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]
2091bp	585bp
pSB1A2

2.RBS+ luxI + terminator

Vector(E+X)	Insert(E+S)
double terminator	RBS+luxI
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
3189bp	579bp
pSB1AK3

3.P(tetR)+RBS+ luxI + terminator

Vector(S+P)	Insert(X+P)
p(tetR)	RBS+luxI+terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
2133bp	726bp
pSB1A2

C₄HSL P(tetR)+rhlI

1.RBS + rhII

Vector(S+P)	Insert(X+P)
RBS	rhII
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]	[http://partsregistry.org/Part:BBa_C0170 BBa_C0170]
2091bp	609bp
pSB1A2

2.RBS + rhII + terminator

Vector(E+X)	Insert(E+S)
double terminator	RBS + rhII
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
3189bp	621bp
pSB1AK3

3.P(tetR) + RBS + rhlI + terminator

Vector(S+P)	Insert(X+P)
p(tetR)	RBS + rhII +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
2625bp	750bp
pSB1A2

AI-1 P(tetR)+lasI

1.RBS + LasI

Vector(S+P)	Insert(X+P)
RBS	LasI
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]	[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0078 BBa_C0078]
2091bp	609bp
pSB1A2

2.RBS + LasI +terminator

Vector(E+X)	Insert(E+S)
double terminator	RBS + LasI +terminator
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
3189bp	654bp
pSB1AK3

3.P(tetR) + RBS + LasI +terminator

Vector(S+P)	Insert(X+P)
p(tetR)	RBS + LasI +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
2091bp	609bp
pSB1A2

3OH,C₁₄:1-HSL P(tetR)+cinI

1. RBS + cinI
K204006

Vector(S+P)	Insert(X+P)
RBS	cinI
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]	[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 BBa_C0076]
2091bp	702bp
pSB1A2

2.RBS + cinI +terminator

Vector(E+X)	Insert(E+S)
double terminator	RBS + cinI
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
3189bp	714bp
pSB1AK3

3.P(tetR) + RBS + cinI +terminator

Vector(S+P)	Insert(X+P)
p(tetR)	RBS + cinI +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
2133bp	843bp
pSB1A2

receiver

signal receiver
 

3O₆HSL luxpL+luxR+luxpR

[http://partsregistry.org/Part:BBa_F2621 BBa_F2621](1158bp)
pSB1A2

C₄HSL p(tetR) + rhlR + RhlR+C₄

1.RhlR+p(Rhl)
K204005

Vector(E+X)	Insert(E+S)
p(RhlR)	RhlR
[http://partsregistry.org/Part:BBa_I14017 BBa_I14017]	[http://partsregistry.org/Part:BBa_I1466 BBa_I1466]
4476bp	884bp
pSB2K3

2.p(tetR)+RhlR+p(Rhl)

Vector(S+P)	Insert(X+P)
p(tetR)	RhlR+p(Rhl)
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
2133bp	935bp
pSB1A2

AI-1 p(tetR) + lasR + LasR+pAI

1. RBS + lasR
K204001,K204004

Vector(S+P)	Insert(X+P)
RBS	lasR
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]	[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0179 BBa_C0179]
2091bp	723bp
pSB1A2

2.RBS + lasR +terminator

Vector(E+X)	Insert(E+S)
double terminator	RBS + lasR +terminator
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
3189bp	735bp
pSB1AK3

3.P(tetR) + RBS + lasR +terminator

Vector(S+P)	Insert(X+P)
p(tetR)	RBS + lasR +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
2625bp	864bp
pSB1A2

4.P(tetR) + RBS + lasR +terminator +lasR+pAI

Vector(E+X)	Insert(E+S)
lasR+pAI	P(tetR) + RBS + lasR +terminator
[http://partsregistry.org/Part:BBa_R0079 BBa_R0079]
2243bp	918bp
pSB1A2

3OH,C₁₄:1-HSL p(tetR) + cinR + cinR

1. RBS + cinR
K204003

Vector(S+P)	Insert(X+P)
RBS	cinR
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]	[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 BBa_C0077]
2091bp	762bp
pSB1A2

2.RBS + cinR +terminator

Vector(E+X)	Insert(E+S)
double terminator	RBS + cinR
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
3189bp	774bp
pSB1AK3

3.P(tetR) + RBS + cinR +terminator

Vector(S+P)	Insert(X+P)
p(tetR)	RBS + cinR +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
2133bp	903bp
pSB1A2

4.P(tetR) + RBS + cinR +terminator +p(cinR)

Vector(E+X)	Insert(E+S)
p(cinR)	P(tetR) + RBS + cinR +terminator
[http://partsregistry.org/Part:BBa_R0078 BBa_R0078]
2304bp	957bp
pSB1A2

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