Team:Paris/ProtocolsMB

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<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Protocols#top | Protocols]] > [[Team:Paris/ProtocolsMB#bottom | Molecular Biology]]
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== Protocols ==
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<center> [[team:Paris/Protocols#Protocols|Main]] - [[Team:Paris/ProtocolsA#Protocols | Microscope Protocol]] - [[Team:Paris/ProtocolsB#Protocols | Adapted Protocols]] - [[Team:Paris/Protocols_Culture#Protocols | Culture protocols]] - [[Team:Paris/ProtocolsMB#Protocols | Molecular Biology]]</center>
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<center>'''Molecular Biology'''</center>
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==PCR with Quick load Taq2x Master Mix==
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Protocols#bottom"> Main </a>|
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<a class="menu_sub" href="https://2009.igem.org/Team:Paris/ProtocolsA#bottom"> Microscope Protocols</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/ProtocolsB#bottom"> Adapted Protocols</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Protocols_Culture#bottom"> Culture Protocols</a>|
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<a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/ProtocolsMB#bottom"> Molecular biology</a>
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==PCR with Quick load Taq2x Master Mix==
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*Mix for 50µl final:
*Mix for 50µl final:
**14µL H2O
**14µL H2O
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**5µL Oligo Rw (0,2µM)
**5µL Oligo Rw (0,2µM)
**1µL Matrice (DNA)
**1µL Matrice (DNA)
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==PCR colonies==
 
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Latest revision as of 15:12, 19 October 2009

iGEM > Paris > Protocols > Molecular Biology


PCR with Quick load Taq2x Master Mix

  • Mix for 50µl final:
    • 14µL H2O
    • 25µL Taq Quick load Master Mix 2X
    • 5µL Oligo Fw (0,2µM)
    • 5µL Oligo Rw (0,2µM)
    • 1µL Matrice (DNA)

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