Template:Team:KULeuven/19 August 2009/VanillinProduction

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(New page: * Miniprep Sam8, Sam5, Ech, Fcs plasmids in triple --> nanodrop concentrations:)
 
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* Miniprep Sam8, Sam5, Ech, Fcs plasmids in triple
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* Miniprep ''sam8, sam5, ech, fcs'' plasmids in triple
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--> nanodrop concentrations:
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* Nanodrop concentrations:
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{| border ="1" align="center"
 +
!| Part || concentration (ng/μl) || 260/280 λ
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|- align="center"
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| Sam8 A ||58,2 || 1,87 ||
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|- align="center"
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| Sam8 B ||63,2 || 1,93 ||
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|- align="center"
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| Sam5 A ||94,6 || 1,92 ||
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|- align="center"
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| Sam5 B ||125,0 || 1,91 ||
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|- align="center"
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| Sam5 C ||104,2 || 1,91 ||
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|- align="center"
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| Ech A ||88,6 || 1,92 ||
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|- align="center"
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| Ech B ||96,7 || 1,95 ||
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|- align="center"
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| Ech C ||105,6 || 1,86 ||
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|- align="center"
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| Fcs A ||84,4 || 1,88 ||
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|- align="center"
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| Fcs B ||113,4 || 1,95 ||
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|- align="center"
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| Fcs C ||90,3 || 1,92 ||
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|-
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|}
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* We took the plasmids with the lowest concentrations to perform the restriction test, described above. Afterwards, we ran the restriction products, together with uncut plasmids, on a gel.
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* Calculations for restriction:
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{| border ="1" align="center"
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!| Part || µl DNA || µl MQ ||
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|- align="center"
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| Sam8 A ||8,7 || 7,3 ||
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|- align="center"
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| Sam5 A ||5,3 || 10,7 || 
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|- align="center"
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| Ech A ||5,6 || 10,4 ||
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|- align="center"
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| Fcs A ||5,9 || 10,1 ||
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|-
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|} 
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 +
 
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*  Meanwhile ... one plated EF colony seemed to have grown. Made fluid culture of EF.

Latest revision as of 11:24, 14 September 2009

  • Miniprep sam8, sam5, ech, fcs plasmids in triple
  • Nanodrop concentrations:
Part concentration (ng/μl) 260/280 λ
Sam8 A 58,2 1,87
Sam8 B 63,2 1,93
Sam5 A 94,6 1,92
Sam5 B 125,0 1,91
Sam5 C 104,2 1,91
Ech A 88,6 1,92
Ech B 96,7 1,95
Ech C 105,6 1,86
Fcs A 84,4 1,88
Fcs B 113,4 1,95
Fcs C 90,3 1,92


  • We took the plasmids with the lowest concentrations to perform the restriction test, described above. Afterwards, we ran the restriction products, together with uncut plasmids, on a gel.
  • Calculations for restriction:
Part µl DNA µl MQ
Sam8 A 8,7 7,3
Sam5 A 5,3 10,7
Ech A 5,6 10,4
Fcs A 5,9 10,1


  • Meanwhile ... one plated EF colony seemed to have grown. Made fluid culture of EF.
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