Template:Team:KULeuven/18 August 2009/BlueLightReceptor
From 2009.igem.org
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- | 1) | + | 1) Miniprep of parts {{kulpart|pSB3K3}}-{{kulpart|BBa_P1010}} and LigA BLP-{{kulpart|BBa_E0240}} |
2) Restriction digest of the DNA from step 1 with EcoRI and PstI | 2) Restriction digest of the DNA from step 1 with EcoRI and PstI | ||
- | 3) | + | 3) Gel electrophoresis to check if the insert in the plasmid is the correct size, which it was, so the ligation was succesful. |
Latest revision as of 09:09, 8 September 2009
1) Miniprep of parts - and LigA BLP-
2) Restriction digest of the DNA from step 1 with EcoRI and PstI
3) Gel electrophoresis to check if the insert in the plasmid is the correct size, which it was, so the ligation was succesful.