Template:Team:KULeuven/18 August 2009/BlueLightReceptor

From 2009.igem.org

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1) miniprep of parts pSB3K3-P1010 and LigA BLP-E0240
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1) Miniprep of parts {{kulpart|pSB3K3}}-{{kulpart|BBa_P1010}} and LigA BLP-{{kulpart|BBa_E0240}}
2) Restriction digest of the DNA from step 1 with EcoRI and PstI
2) Restriction digest of the DNA from step 1 with EcoRI and PstI
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3) gelelectrophoresis to check if the insert in the plasmid is the correct size, which it was, so the ligation was succesfull.
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3) Gel electrophoresis to check if the insert in the plasmid is the correct size, which it was, so the ligation was succesful.

Latest revision as of 09:09, 8 September 2009

1) Miniprep of parts - and LigA BLP-

2) Restriction digest of the DNA from step 1 with EcoRI and PstI

3) Gel electrophoresis to check if the insert in the plasmid is the correct size, which it was, so the ligation was succesful.