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August/19 August 2009
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(New page: 1.<B>before Min prep</b><br> Checked the cell cultures transformed and plated out yesterday (8/10) for colony formation and made an approximate count of the number of colonies. (sample ...) |
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Latest revision as of 07:55, 12 October 2009
1.before Min prep
Checked the cell cultures transformed and plated out yesterday (8/10) for colony formation and made an approximate count of the number of colonies.
(sample no.) (no. of colonies)
③ 10 (Amp)
④ 50~ (Amp)
⑤ 1 (Kan)
⑥ ~10 (Amp)
4 colony (each ③,④,⑥) inoculate to iquid medium(5mL + Amp 5uL)
2. 0.1M MgCl2, 0.1M CaCl2 buffer
