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August/19 August 2009
From 2009.igem.org
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2. 0.1M MgCl<sub>2</sub>, 0.1M CaCl<sub>2</sub> buffer | 2. 0.1M MgCl<sub>2</sub>, 0.1M CaCl<sub>2</sub> buffer | ||
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| + | [https://2009.igem.org/Team:Osaka/NOTES back to NOTES] | ||
Latest revision as of 07:55, 12 October 2009
1.before Min prep
Checked the cell cultures transformed and plated out yesterday (8/10) for colony formation and made an approximate count of the number of colonies.
(sample no.) (no. of colonies)
③ 10 (Amp)
④ 50~ (Amp)
⑤ 1 (Kan)
⑥ ~10 (Amp)
4 colony (each ③,④,⑥) inoculate to iquid medium(5mL + Amp 5uL)
2. 0.1M MgCl2, 0.1M CaCl2 buffer
