Template:Team:KULeuven/21 August 2009/VanillinProduction

From 2009.igem.org

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* Test 2: We loaded yesterday's restrictions on an agarose gel. It showed that the enzymes cut properly.
+
* <u>Test 2</u>: We loaded yesterday's restrictions on an agarose gel. It showed that the enzymes cut properly.
-
However, the EF results were unexpected. It seems that somehow the bacteria had taken in a self-closed Ech-plasmid. The signals do not correspond with an EF-ligation product.
+
However, the EF results were unexpected. It seems that somehow the bacteria had taken in a self-closed ''ech''-plasmid. The signals do not correspond with an EF-ligation product.
* The DNA was purified from the gel  
* The DNA was purified from the gel  
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!| Part || ng/µl || 260/280 ||
!| Part || ng/µl || 260/280 ||
|- align="center"
|- align="center"
-
| Sam8 ||2,5 || 1,14 ||  
+
| ''sam8'' ||2,5 || 1,14 ||  
|- align="center"
|- align="center"
-
| Sam5 ||7,9 || 1,60 ||   
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| ''sam5'' ||7,9 || 1,60 ||   
|- align="center"
|- align="center"
-
| Ech ||5,0 || 1,38 ||  
+
| ''ech'' ||5,0 || 1,38 ||  
|- align="center"
|- align="center"
-
| Fcs ||3,4 || 0,93 ||  
+
| ''fcs'' ||3,4 || 0,93 ||  
|-
|-
|}   
|}   
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* Sam8 and Sam5; Fcs and Ech were ligated
+
* ''sam8'' and ''sam5''; ''fcs'' and ''ech'' were ligated
Used volumes:
Used volumes:
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!| Part || µl ||  
!| Part || µl ||  
|- align="center"
|- align="center"
-
| Sam8 ||34 ||   
+
| ''sam8'' ||34 ||   
|- align="center"
|- align="center"
-
| Sam5 ||6,3 ||   
+
| ''sam5'' ||6,3 ||   
|- align="center"
|- align="center"
-
| Ech ||10,0 ||   
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| ''ech'' ||10,0 ||   
|- align="center"
|- align="center"
-
| Fcs ||35,8 ||  
+
| ''fcs'' ||35,8 ||  
|-
|-
|}
|}

Latest revision as of 11:39, 14 September 2009

  • Test 2: We loaded yesterday's restrictions on an agarose gel. It showed that the enzymes cut properly.

However, the EF results were unexpected. It seems that somehow the bacteria had taken in a self-closed ech-plasmid. The signals do not correspond with an EF-ligation product.

  • The DNA was purified from the gel

Nanodrop results:

Part ng/µl 260/280
sam8 2,5 1,14
sam5 7,9 1,60
ech 5,0 1,38
fcs 3,4 0,93
  • sam8 and sam5; fcs and ech were ligated

Used volumes:

Part µl
sam8 34
sam5 6,3
ech 10,0
fcs 35,8
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