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Team:TUDelft/27 August 2009
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| + | {{Template:TUDelftiGEM2009}} | ||
| + | {{Template:TUDelftiGEM2009_LabNotebook}} | ||
| + | |||
| + | ='''27 August 2009'''= | ||
| + | ===Sriram=== | ||
| + | Yesterday's gel is shown below. I continued the ligation and electro-transformation of assemblies. I will check the colonies tomorrow and I will be back to lab only on 1st September since tomorrow and 31st August I will be on leave for mentoring job and US visa interview. | ||
| + | [[Image:gel270809-Delay Ribo Assembly II 5678TT.jpg|thumb|center|450px]] | ||
===Saeed=== | ===Saeed=== | ||
| Line 5: | Line 12: | ||
The overnight colonies were used for miniprep. | The overnight colonies were used for miniprep. | ||
!Za, !Zb, !Zg and !Zh had the highest DNA concentrations. These were downstream cut and checked on 1% agarose gel for fragment size. Fragment should be 1934 bp. | !Za, !Zb, !Zg and !Zh had the highest DNA concentrations. These were downstream cut and checked on 1% agarose gel for fragment size. Fragment should be 1934 bp. | ||
| + | |||
| + | [[Image:Gelsaeed27-082009_%21Z.wiki.jpg|center|450px]] | ||
| + | |||
| + | {| border="1" align="center" | ||
| + | | lane || Part || Expected Plasmid Size || Status | ||
| + | |- align="center" | ||
| + | | 1 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] || || | ||
| + | |- align="center" | ||
| + | | 2 || !Za || 1934 || no band | ||
| + | |- align="center" | ||
| + | | 3 || !Zb || 1934 || ok | ||
| + | |- align="center" | ||
| + | | 4 || !Zg || 1934 || ok | ||
| + | |- align="center" | ||
| + | | 5 || !Zh || 1934 || ok | ||
| + | |- align="center" | ||
| + | | 6 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] || || | ||
| + | |} | ||
| + | |||
| + | !Zb and !Zg seems to be ok. !Zh looks a bit bigger, but this also could be because of the gel. !Zb or !Zg should be used for sequencing. | ||
| + | |||
| + | ===Daniel=== | ||
| + | Miniprep of locks and keys in biobrick´s plasmid backbones and some previous assemblies | ||
| + | |||
| + | {| border="1" align="center" | ||
| + | | Part || [DNA] ng/uL | ||
| + | |- align="center" | ||
| + | | LMA || 46.1 | ||
| + | |- align="center" | ||
| + | | KMA || 58.8 | ||
| + | |- align="center" | ||
| + | | LWA || 59.1 | ||
| + | |- align="center" | ||
| + | | KWA || 54.7 | ||
| + | |- align="center" | ||
| + | | 2B || 64.4 | ||
| + | |- align="center" | ||
| + | | 1A || 122.2 | ||
| + | |- align="center" | ||
| + | | 1B || 113.3 | ||
| + | |} | ||
| + | |||
| + | Restriction of Locks and keys to use them for assembly. The gel shows weird results. | ||
| + | |||
| + | [[Image:gel280809.jpg|550px]] | ||
| + | |||
| + | Ligation of 1C, 2C, 3C and 4C | ||
{{Template:TUDelftiGEM2009_end}} | {{Template:TUDelftiGEM2009_end}} | ||
Latest revision as of 20:40, 20 October 2009
Lab Notebook
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27 August 2009
Sriram
Yesterday's gel is shown below. I continued the ligation and electro-transformation of assemblies. I will check the colonies tomorrow and I will be back to lab only on 1st September since tomorrow and 31st August I will be on leave for mentoring job and US visa interview.
Saeed
The overnight colonies were used for miniprep. !Za, !Zb, !Zg and !Zh had the highest DNA concentrations. These were downstream cut and checked on 1% agarose gel for fragment size. Fragment should be 1934 bp.
| lane | Part | Expected Plasmid Size | Status |
| 1 | [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] | ||
| 2 | !Za | 1934 | no band |
| 3 | !Zb | 1934 | ok |
| 4 | !Zg | 1934 | ok |
| 5 | !Zh | 1934 | ok |
| 6 | [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] |
!Zb and !Zg seems to be ok. !Zh looks a bit bigger, but this also could be because of the gel. !Zb or !Zg should be used for sequencing.
Daniel
Miniprep of locks and keys in biobrick´s plasmid backbones and some previous assemblies
| Part | [DNA] ng/uL |
| LMA | 46.1 |
| KMA | 58.8 |
| LWA | 59.1 |
| KWA | 54.7 |
| 2B | 64.4 |
| 1A | 122.2 |
| 1B | 113.3 |
Restriction of Locks and keys to use them for assembly. The gel shows weird results.
Ligation of 1C, 2C, 3C and 4C
