Virginia Commonwealth/10 June 2009
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==Wednesday 10 June 2009== | ==Wednesday 10 June 2009== | ||
===Results=== | ===Results=== | ||
- | * | + | * n/a |
- | + | ||
---- | ---- | ||
===Tasks=== | ===Tasks=== | ||
+ | * Transform and plate promoter characterization parts. | ||
+ | * Request Parts | ||
+ | {| cellpadding="5" border="1" padding="5" | ||
+ | |+ | ||
+ | |- | ||
+ | ! Parts to be Ordered | ||
+ | |- | ||
+ | |BBa_J23150 | ||
+ | |- | ||
+ | |BBa_J23151 | ||
+ | |} | ||
+ | |||
---- | ---- | ||
- | |||
- | |||
====Wetlab==== | ====Wetlab==== | ||
- | *1 | + | * Transformed the following plasmids into Electrocompetent NEB10β |
- | + | {| cellpadding="5" border="1" padding="5" | |
+ | |+ | ||
+ | |- | ||
+ | ! Parts Transformed !! Plate !! Desired Vector Part | ||
+ | |- | ||
+ | |pSB3K3 || KAN || Backbone | ||
+ | |- | ||
+ | |BBa_E0240 || AMP || RET | ||
+ | |- | ||
+ | |BBa_J23101 || AMP || Reference Promoter | ||
+ | |- | ||
+ | |BBa_J23113 || AMP || Promoter | ||
+ | |- | ||
+ | |BBa_J23116 || AMP || Promoter | ||
+ | |- | ||
+ | |BBa_J23102 || AMP || Promoter | ||
+ | |- | ||
+ | |BBa_R0040 || AMP || Promoter | ||
+ | |- | ||
+ | |BBa_R0011 || AMP || Promoter | ||
+ | |- | ||
+ | |BBa_J23100 || AMP || Promoter | ||
+ | |- | ||
+ | |BBa_J23103 || AMP || Promoter | ||
+ | |- | ||
+ | |BBa_J23104 || AMP || Promoter | ||
+ | |- | ||
+ | |BBa_J23105 || AMP || Promoter | ||
+ | |- | ||
+ | |BBa_J23106 || AMP || Promoter | ||
+ | |- | ||
+ | |BBa_J23107 || AMP || Promoter | ||
+ | |- | ||
+ | |BBa_J23110 || AMP || Promoter | ||
+ | |} | ||
+ | |||
+ | {| cellpadding="5" border="1" padding="5" | ||
+ | |+ | ||
+ | |- | ||
+ | ! Control !! Plate | ||
+ | |- | ||
+ | | (+) Control || LB | ||
+ | |- | ||
+ | | (-) Control shocked || AMP | ||
+ | |- | ||
+ | | (-) Control shocked || Cm | ||
+ | |- | ||
+ | | (-) Control shocked || KAN | ||
+ | |} |
Latest revision as of 15:38, 5 August 2009
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Contents |
Wednesday 10 June 2009
Results
- n/a
Tasks
- Transform and plate promoter characterization parts.
- Request Parts
Parts to be Ordered |
---|
BBa_J23150 |
BBa_J23151 |
Wetlab
- Transformed the following plasmids into Electrocompetent NEB10β
Parts Transformed | Plate | Desired Vector Part |
---|---|---|
pSB3K3 | KAN | Backbone |
BBa_E0240 | AMP | RET |
BBa_J23101 | AMP | Reference Promoter |
BBa_J23113 | AMP | Promoter |
BBa_J23116 | AMP | Promoter |
BBa_J23102 | AMP | Promoter |
BBa_R0040 | AMP | Promoter |
BBa_R0011 | AMP | Promoter |
BBa_J23100 | AMP | Promoter |
BBa_J23103 | AMP | Promoter |
BBa_J23104 | AMP | Promoter |
BBa_J23105 | AMP | Promoter |
BBa_J23106 | AMP | Promoter |
BBa_J23107 | AMP | Promoter |
BBa_J23110 | AMP | Promoter |
Control | Plate |
---|---|
(+) Control | LB |
(-) Control shocked | AMP |
(-) Control shocked | Cm |
(-) Control shocked | KAN |