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August/31 August 2009
From 2009.igem.org
(New page: 1.Min prep <br> filure<br> <br> 2.ligation check <br>) |
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| - | 1. | + | 1. Miniprep of '''(12)-B, (13)-A, (14)-A, (15)-A, (16)-A, (19)-A''' solution cultures inoculated from streak plates on 8/30.<br> Failed due to mistake in procedure; the above numbered parts were again inoculated from streak plates into culture solutions for miniprep tomorrow.<br> |
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| - | + | 2. Ligation check of '''(12) through (16), (18) and (19)''' <- all miniprepped on 8/30; checking was done by taking a small sample for electrophoresis and length comparison.<br> | |
| + | [[Image:Osaka090831.jpg]] | ||
| + | UP : No.12 , 15 ,16 , Vector , 13 , 14 , 18 , 19 , Vector , ladder | ||
| + | DOWN : No.20-I,V , ladder , No.21-I,V | ||
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| + | Results for '''(14), (18), (19)''' were not satisfactory; '''the others''' seemed okay and may be established as completed parts.<br> | ||
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| + | 3. Repeat of ligation assembly of '''(20) and (21)''': today their constituent parts were treated with restriction enzymes, electrophoresed and purified.<br> Tomorrow they will be treated with ligation enzymes followed by transformation. | ||
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| + | [https://2009.igem.org/Team:Osaka/NOTES back to NOTES] | ||
Latest revision as of 08:25, 12 October 2009
1. Miniprep of (12)-B, (13)-A, (14)-A, (15)-A, (16)-A, (19)-A solution cultures inoculated from streak plates on 8/30.
Failed due to mistake in procedure; the above numbered parts were again inoculated from streak plates into culture solutions for miniprep tomorrow.
2. Ligation check of (12) through (16), (18) and (19) <- all miniprepped on 8/30; checking was done by taking a small sample for electrophoresis and length comparison.
UP : No.12 , 15 ,16 , Vector , 13 , 14 , 18 , 19 , Vector , ladder DOWN : No.20-I,V , ladder , No.21-I,V
Results for (14), (18), (19) were not satisfactory; the others seemed okay and may be established as completed parts.
3. Repeat of ligation assembly of (20) and (21): today their constituent parts were treated with restriction enzymes, electrophoresed and purified.
Tomorrow they will be treated with ligation enzymes followed by transformation.
