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Template:Team:KULeuven/13 August 2009/BlueLightReceptor
From 2009.igem.org
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# Restriction digest {{kulpart|BBa_K145015}} cut with EcoRI and XbaI | # Restriction digest {{kulpart|BBa_K145015}} cut with EcoRI and XbaI | ||
| - | # Electroporation of | + | # Electroporation of {{kulpart|pSB3K3}} + {{kulpart|BBa_P1010}} and the ligation of ... |
| - | # | + | # Gelelectrophoresis of {{Kulpart|BBa_K145015}} |
#* Only 1 band, so probably everything was cut | #* Only 1 band, so probably everything was cut | ||
| - | # | + | # Gelextraction of {{Kulpart|BBa_K145015}} |
#* Nanodrop = 29,3 ng/μl | #* Nanodrop = 29,3 ng/μl | ||
| - | # | + | #Ligation of vector {{Kulpart|BBa_K145015}} and insert |
Latest revision as of 08:57, 8 September 2009
- PCR product of 12-august of pairt
- Miniprep of
Nanodrop results:
| Part | concentration (ng/μl) | 260/280 λ | 260/230 λ |
|---|---|---|---|
| 123,3 | 1,82 | ||
| 141,2 | 1,99 |
- Restriction digest cut with EcoRI and XbaI
- Electroporation of + and the ligation of ...
- Gelelectrophoresis of
- Only 1 band, so probably everything was cut
- Gelextraction of
- Nanodrop = 29,3 ng/μl
- Ligation of vector and insert
