Template:Team:KULeuven/12 August 2009/BlueLightReceptor

From 2009.igem.org

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# Gel electrophorese of BlP cut with EcoRI and SpeI
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# Gel electrophoresis of BlP cut with EcoRI and SpeI
# Gel extraction of the Blp  
# Gel extraction of the Blp  
Nanodrop results
Nanodrop results
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# PCR (with Pfx) of blue light promoter with primers iGEM 2260 and igem 2261
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# PCR (with Pfx) of blue light promoter with primers iGEM 2260 and iGEM 2261
#* Annealing of 58C
#* Annealing of 58C
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# Innoculate liquid medium
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# Inoculate liquid medium
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#* Innoculation of Psb3k3-P1010 and J23101
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#* Inoculation of {{kulpart|pSB3K3}}-{{kulpart|BBa_P1010}} and {{kulpart|BBa_J23101}}
# Ligation  
# Ligation  
{| border ="1" align="center"
{| border ="1" align="center"
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| Vector || insert  
| Vector || insert  
|- align="center"
|- align="center"
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| {{kulpart|psB1A3}}+P1010 || {{kulpart|BBa_K238013}} (BLP)  
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| {{kulpart|pSB1A3}}+{{kulpart|BBa_P1010}} || {{kulpart|BBa_K238013}} (BLP)  
|- align="center"
|- align="center"
| 50ng -> 2,5 μl || 5 ng -> 0,3 μl  
| 50ng -> 2,5 μl || 5 ng -> 0,3 μl  
|- align="center"
|- align="center"
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Latest revision as of 08:48, 8 September 2009

  1. Gel electrophoresis of BlP cut with EcoRI and SpeI
  2. Gel extraction of the Blp

Nanodrop results

Part concentration (ng/μl) 260/280 λ 260/230 λ
Blp 27 2,08
  1. PCR (with Pfx) of blue light promoter with primers iGEM 2260 and iGEM 2261
    • Annealing of 58C
  2. Inoculate liquid medium
    • Inoculation of - and
  3. Ligation
Vector insert
+ (BLP)
50ng -> 2,5 μl 5 ng -> 0,3 μl
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