Template:Team:KULeuven/25 August 2009/BlueLightReceptor

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* miniprepping the liquid cultures with {{kulpart|pSB3K3}}
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* Miniprepping the liquid cultures with {{kulpart|pSB3K3}}
{| border ="1" align="center"
{| border ="1" align="center"
!| Part || concentration (ng/μl) || 260/280 λ
!| Part || concentration (ng/μl) || 260/280 λ
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| {{kulpart|pSB3K3}} (2) || 47,7 || 2,08
| {{kulpart|pSB3K3}} (2) || 47,7 || 2,08
|}
|}
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*restriction digest of {{kulpart|pSB3K3}} with EcoRI and PstI
+
*Restriction digest of {{kulpart|pSB3K3}} with EcoRI and PstI
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*gel electroforesis and extraction of the RD of {{kulpart|pSB3K3}}
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*Gel electrophoresis and extraction of the RD of {{kulpart|pSB3K3}}
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--> failed. there was no signal at all
+
--> failed. There was no signal at all
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*new colonies were ented in liquid culture.
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*New colonies were ented in liquid culture.
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*ligation C performed: {{kulpart|BBa_J23101}} (A) + {{kulpart|BBa_E0240}}
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*Ligation C performed: {{kulpart|BBa_J23101}} (A) + {{kulpart|BBa_E0240}}
{| border ="1" align="center"
{| border ="1" align="center"
|- align="center"
|- align="center"

Latest revision as of 12:30, 14 September 2009

  • Miniprepping the liquid cultures with
Part concentration (ng/μl) 260/280 λ
(1) 27,6 1,91
(2) 47,7 2,08
  • Restriction digest of with EcoRI and PstI
  • Gel electrophoresis and extraction of the RD of

--> failed. There was no signal at all

  • New colonies were ented in liquid culture.
  • Ligation C performed: (A) +
Vector insert
+
30ng -> 1,4μl 40,8ng -> 2μl