"
Team:KULeuven/24 July 2009
From 2009.igem.org
Bart Bosmans (Talk | contribs) |
|||
| (One intermediate revision not shown) | |||
| Line 1: | Line 1: | ||
| - | {{Team:KULeuven/ | + | {{Team:KULeuven/Common2/BeginHeader}} |
{{Team:KULeuven/Common/SubMenu_Notebook}} | {{Team:KULeuven/Common/SubMenu_Notebook}} | ||
| - | {{Team:KULeuven/ | + | {{Team:KULeuven/Common2/EndHeader}} |
{{Team:KULeuven/Notebook/DayNavigator}} | {{Team:KULeuven/Notebook/DayNavigator}} | ||
=Progress= | =Progress= | ||
| - | * Received E. Coli | + | * Received E. Coli odourless strain {{kulpart|BBa_J45999}}, put in 4C |
==Vanillin Receptor== | ==Vanillin Receptor== | ||
| - | Possible receptor is the VirA/VirG system from Agrobacterium, the VirA protein will bind to phenolic compounds and induce transcription of vir genes by phosphorylation of VirG. | + | Possible receptor is the VirA/VirG system from ''Agrobacterium'', the VirA protein will bind to phenolic compounds and induce transcription of ''vir'' genes by phosphorylation of VirG. Protein ChvE results in a broader response to phenolic compounds. Papers: |
[[http://mmbr.asm.org/cgi/content/abstract/56/1/12 1]] S C Winans, Two-way chemical signaling in Agrobacterium-plant interactions, Microbiol Mol Biol Rev. 1992 March; 56(1): 12-31 <br> | [[http://mmbr.asm.org/cgi/content/abstract/56/1/12 1]] S C Winans, Two-way chemical signaling in Agrobacterium-plant interactions, Microbiol Mol Biol Rev. 1992 March; 56(1): 12-31 <br> | ||
| Line 22: | Line 22: | ||
* ech; RBS + ech, in pSB1A2, {{kulpart|BBa_I742113}} | * ech; RBS + ech, in pSB1A2, {{kulpart|BBa_I742113}} | ||
* fcs; RBS + fcs, in pSB1A2, {{kulpart|BBa_I742115}} | * fcs; RBS + fcs, in pSB1A2, {{kulpart|BBa_I742115}} | ||
| + | {{Team:KULeuven/Common2/PageFooter}} | ||
Latest revision as of 08:40, 10 September 2009
Progress
- Received E. Coli odourless strain , put in 4C
Vanillin Receptor
Possible receptor is the VirA/VirG system from Agrobacterium, the VirA protein will bind to phenolic compounds and induce transcription of vir genes by phosphorylation of VirG. Protein ChvE results in a broader response to phenolic compounds. Papers:
http://mmbr.asm.org/cgi/content/abstract/56/1/12 1 S C Winans, Two-way chemical signaling in Agrobacterium-plant interactions, Microbiol Mol Biol Rev. 1992 March; 56(1): 12-31
http://jb.asm.org/cgi/content/abstract/180/21/5632 2 Wen-Tao Peng, Yong-Woog Lee, and Eugene W. Nester, The Phenolic Recognition Profiles of the Agrobacterium tumefaciens VirA Protein Are Broadened by a High Level of the Sugar Binding Protein ChvE, Journal of Bacteriology, November 1998, p. 5632-5638, Vol. 180, No. 21
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T39-3W269GC-2C&_user=877992&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000047079&_version=1&_urlVersion=0&_userid=877992&md5=95a48c4a2e3cf93948ab45c9a92e78a6 3Yong-Woog Lee, Shouguang Jin, Woong-Seop Sim, Eugene W. Nester, The sensing of plant signal molecules by Agrobacterium: genetic evidence for direct recognition of phenolic inducers by the VirA protein, Gene, Volume 179, Issue 1, 1996, Pages 83-88, ISSN 0378-1119, DOI: 10.1016/S0378-1119(96)00328-9.
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=40333 4Y W Lee, S Jin, W S Sim, and E W Nester,Genetic evidence for direct sensing of phenolic compounds by the VirA protein of Agrobacterium tumefaciens, Proc Natl Acad Sci U S A. 1995 December 19; 92(26): 12245–12249.
Vanillin synthesis
Received mail from U. Edinburgh: We will receive:
- Sam5; no part number, Pst1 site removed
- Sam8; coding sequence without RBS
- ComT; coding sequence without RBS
- ech; RBS + ech, in pSB1A2,
- fcs; RBS + fcs, in pSB1A2,
