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Template:Team:KULeuven/4 September 2009/VanillinProduction
From 2009.igem.org
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* The restriction digest from SAMS and the terminator was put on gel | * The restriction digest from SAMS and the terminator was put on gel | ||
| - | ** It appears there is no SAMS in between the vector, the length is approx. 1500 bp which can only be | + | ** It appears there is no SAMS in between the vector, the length is approx. 1500 bp which can only be ''sam5'' or'' sam8'' |
* Electroporation of the ligation EF | * Electroporation of the ligation EF | ||
| - | ** Plated and put | + | ** Plated and put at 37°C |
| + | * Liquid cultures made from ''sam5, sam8, ech'' and ''fcs''. | ||
Latest revision as of 12:02, 14 September 2009
- The restriction digest from SAMS and the terminator was put on gel
- It appears there is no SAMS in between the vector, the length is approx. 1500 bp which can only be sam5 or sam8
- Electroporation of the ligation EF
- Plated and put at 37°C
- Liquid cultures made from sam5, sam8, ech and fcs.
