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Template:Team:KULeuven/6 September 2009/VanillinProduction
From 2009.igem.org
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JochemDeen (Talk | contribs) (New page: * Restriction digest overnight of Sam5 (1,2) and Sam8 (1,2)) |
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| - | * Restriction digest overnight of | + | * Restriction digest overnight of ''sam5'' (1,2) and ''sam8'' (1,2) |
| + | * Growth of EF Ligation (woohoo!) | ||
| + | ** Transferred 4 colonies from EF ligation to a new plate | ||
| + | ** Put the 4 transferred colonies on liquid medium | ||
| + | A miniprep and restriction digest (ON) of ''sam5, sam8, ech'' and ''fcs'' was performed on Saturday. On Sunday, a gel was run to check the inserts. These were good so a motherplate for all the genes could be made. | ||
Latest revision as of 12:04, 14 September 2009
- Restriction digest overnight of sam5 (1,2) and sam8 (1,2)
- Growth of EF Ligation (woohoo!)
- Transferred 4 colonies from EF ligation to a new plate
- Put the 4 transferred colonies on liquid medium
A miniprep and restriction digest (ON) of sam5, sam8, ech and fcs was performed on Saturday. On Sunday, a gel was run to check the inserts. These were good so a motherplate for all the genes could be made.
