Team:UNIPV-Pavia/Freezer
From 2009.igem.org
(Difference between revisions)
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<td align="center" width="165px" valign="center"><font face="papyrus" color="royalblue"><b>Description</td> | <td align="center" width="165px" valign="center"><font face="papyrus" color="royalblue"><b>Description</td> | ||
<td align="center" width="73px" valign="center"><font face="papyrus" color="royalblue"><b>Creation Date</td> | <td align="center" width="73px" valign="center"><font face="papyrus" color="royalblue"><b>Creation Date</td> | ||
- | |||
<td align="center" width="60px" valign="center"><font face="papyrus" color="royalblue"><b>Strain</td> | <td align="center" width="60px" valign="center"><font face="papyrus" color="royalblue"><b>Strain</td> | ||
<td align="center" width="97px" valign="center"><font face="papyrus" color="royalblue"><b>Vector</td> | <td align="center" width="97px" valign="center"><font face="papyrus" color="royalblue"><b>Vector</td> | ||
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<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 49: | Line 47: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 57: | Line 54: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 65: | Line 61: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 73: | Line 68: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 81: | Line 75: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 89: | Line 82: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 97: | Line 89: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 105: | Line 96: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 113: | Line 103: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 121: | Line 110: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 129: | Line 117: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 137: | Line 124: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 145: | Line 131: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 153: | Line 138: | ||
<td class="prova">GFP protein generator<br>(RBS-GFP-TT)</td> | <td class="prova">GFP protein generator<br>(RBS-GFP-TT)</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 161: | Line 145: | ||
<td class="prova">colE2 operon:ceaB-ceiB-Tweak-celB-T (with RBS)</td> | <td class="prova">colE2 operon:ceaB-ceiB-Tweak-celB-T (with RBS)</td> | ||
<td class="prova">May 28</td> | <td class="prova">May 28</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1AK3</td> | <td class="prova">pSB1AK3</td> | ||
Line 169: | Line 152: | ||
<td class="prova">colE7 lysis gene</td> | <td class="prova">colE7 lysis gene</td> | ||
<td class="prova">May 28</td> | <td class="prova">May 28</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 177: | Line 159: | ||
<td class="prova">Phage Lysis Cassette</td> | <td class="prova">Phage Lysis Cassette</td> | ||
<td class="prova">May 28</td> | <td class="prova">May 28</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1AK3</td> | <td class="prova">pSB1AK3</td> | ||
Line 185: | Line 166: | ||
<td class="prova">Double terminator</td> | <td class="prova">Double terminator</td> | ||
<td class="prova">May 28</td> | <td class="prova">May 28</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1AK3</td> | <td class="prova">pSB1AK3</td> | ||
Line 193: | Line 173: | ||
<td class="prova">RBS-lacZ</td> | <td class="prova">RBS-lacZ</td> | ||
<td class="prova">May 28</td> | <td class="prova">May 28</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 201: | Line 180: | ||
<td class="prova">RBS 0.6</td> | <td class="prova">RBS 0.6</td> | ||
<td class="prova">Jun 4</td> | <td class="prova">Jun 4</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 209: | Line 187: | ||
<td class="prova">RBS 0.07</td> | <td class="prova">RBS 0.07</td> | ||
<td class="prova">Jun 4</td> | <td class="prova">Jun 4</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 217: | Line 194: | ||
<td class="prova">RBS 0.3</td> | <td class="prova">RBS 0.3</td> | ||
<td class="prova">Jun 4</td> | <td class="prova">Jun 4</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 225: | Line 201: | ||
<td class="prova">RBS 0.01</td> | <td class="prova">RBS 0.01</td> | ||
<td class="prova">Jun 4</td> | <td class="prova">Jun 4</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 233: | Line 208: | ||
<td class="prova">RBS 1</td> | <td class="prova">RBS 1</td> | ||
<td class="prova">Jun 4</td> | <td class="prova">Jun 4</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 241: | Line 215: | ||
<td class="prova">RBS-tetR-TT</td> | <td class="prova">RBS-tetR-TT</td> | ||
<td class="prova">Jun 4</td> | <td class="prova">Jun 4</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 249: | Line 222: | ||
<td class="prova">Ptet</td> | <td class="prova">Ptet</td> | ||
<td class="prova">Jun 4</td> | <td class="prova">Jun 4</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 257: | Line 229: | ||
<td class="prova">Ptet-RBS-luxR-TT-Plux-RBS-GFP-TT</td> | <td class="prova">Ptet-RBS-luxR-TT-Plux-RBS-GFP-TT</td> | ||
<td class="prova">Jun 4</td> | <td class="prova">Jun 4</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A3</td> | <td class="prova">pSB1A3</td> | ||
Line 265: | Line 236: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">Jun 4</td> | <td class="prova">Jun 4</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 273: | Line 243: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">Jun 4</td> | <td class="prova">Jun 4</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 281: | Line 250: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">Jun 4</td> | <td class="prova">Jun 4</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
<td class="prova">sequence ok (MIT)</td> | <td class="prova">sequence ok (MIT)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A1</td> | + | <tr class="in_freezer"><td class="prova">A1 (BBa_K173000)</td> |
<td class="prova">J23100(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td> | <td class="prova">J23100(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td> | ||
<td class="prova">Jun 11</td> | <td class="prova">Jun 11</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a></td> | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a></td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A2</td> | + | <tr class="in_freezer"><td class="prova">A2 (BBa_K173001)</td> |
<td class="prova">J23101(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td> | <td class="prova">J23101(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td> | ||
<td class="prova">Jun 11</td> | <td class="prova">Jun 11</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a></td> | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a></td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A3-1</td> | + | <tr class="in_freezer"><td class="prova">A3-1 (BBa_K173004)</td> |
<td class="prova">I732017-B0015<br>(RBS-lacZ-TT)</td> | <td class="prova">I732017-B0015<br>(RBS-lacZ-TT)</td> | ||
<td class="prova">Jun 12</td> | <td class="prova">Jun 12</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1AK3</td> | <td class="prova">pSB1AK3</td> | ||
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_digestion_A3_B0015.jpg">(digestion PstI)</a> sequence ok -long part- (BMR)</td> | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_digestion_A3_B0015.jpg">(digestion PstI)</a> sequence ok -long part- (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A4</td> | + | <tr class="in_freezer"><td class="prova">A4 (BBa_K173005)</td> |
<td class="prova">P0440-R0040<br>(RBS-tetR-TT-Ptet)</td> | <td class="prova">P0440-R0040<br>(RBS-tetR-TT-Ptet)</td> | ||
<td class="prova">Jun 11</td> | <td class="prova">Jun 11</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a> sequence ok (BMR)</td> | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a> sequence ok (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A5</td> | + | <tr class="in_freezer"><td class="prova">A5 (BBa_K173006)</td> |
<td class="prova">A4-A3<br>(RBS-tetR-TT-Ptet-RBS-lacZ-TT)</td> | <td class="prova">A4-A3<br>(RBS-tetR-TT-Ptet-RBS-lacZ-TT)</td> | ||
<td class="prova">Jun 19</td> | <td class="prova">Jun 19</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1AK3</td> | <td class="prova">pSB1AK3</td> | ||
<td class="prova">sequence ok -long part- (BMR)</td> | <td class="prova">sequence ok -long part- (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A6</td> | + | <tr class="in_freezer"><td class="prova">A6 (BBa_K173007)</td> |
<td class="prova">J23100(Jun 4)-A4<br>(Pcon-RBS-tetR-TT-Ptet)</td> | <td class="prova">J23100(Jun 4)-A4<br>(Pcon-RBS-tetR-TT-Ptet)</td> | ||
<td class="prova">Jun 19</td> | <td class="prova">Jun 19</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 337: | Line 299: | ||
<td class="prova">J23118-B0034-C0012-B0015<br>(Pcon-RBS-lacI-TT)</td> | <td class="prova">J23118-B0034-C0012-B0015<br>(Pcon-RBS-lacI-TT)</td> | ||
<td class="prova">Jun 23</td> | <td class="prova">Jun 23</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">sequence ok (BMR)</td> | <td class="prova">sequence ok (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A7</td> | + | <tr class="in_freezer"><td class="prova">A7 (BBa_K173002)</td> |
<td class="prova">J23118(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td> | <td class="prova">J23118(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td> | ||
<td class="prova">Jun 25</td> | <td class="prova">Jun 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 353: | Line 313: | ||
<td class="prova">Plac</td> | <td class="prova">Plac</td> | ||
<td class="prova">Jun 25</td> | <td class="prova">Jun 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 361: | Line 320: | ||
<td class="prova">holin-lysozyme-T-Pconweak-antiholin-T (with RBSs)</td> | <td class="prova">holin-lysozyme-T-Pconweak-antiholin-T (with RBSs)</td> | ||
<td class="prova">Jun 25</td> | <td class="prova">Jun 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 369: | Line 327: | ||
<td class="prova">A4-E0240<br>(RBS-tetR-TT-Ptet-RBS-GFP-TT)</td> | <td class="prova">A4-E0240<br>(RBS-tetR-TT-Ptet-RBS-GFP-TT)</td> | ||
<td class="prova">Jun 29</td> | <td class="prova">Jun 29</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 377: | Line 334: | ||
<td class="prova">A6-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td> | <td class="prova">A6-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td> | ||
<td class="prova">Jun 29</td> | <td class="prova">Jun 29</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 385: | Line 341: | ||
<td class="prova">K117000-B0015<br>(celB-TT)</td> | <td class="prova">K117000-B0015<br>(celB-TT)</td> | ||
<td class="prova">Jun 30</td> | <td class="prova">Jun 30</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1AK3</td> | <td class="prova">pSB1AK3</td> | ||
<td class="prova">sequence BAD (BMR)</td> | <td class="prova">sequence BAD (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A8pg</td> | + | <tr class="in_freezer"><td class="prova">A8pg (BBa_K173008)</td> |
<td class="prova">A4-E0240<br>(RBS-tetR-TT-Ptet-RBS-GFP-TT)</td> | <td class="prova">A4-E0240<br>(RBS-tetR-TT-Ptet-RBS-GFP-TT)</td> | ||
<td class="prova">Jul 3</td> | <td class="prova">Jul 3</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9_10pg.jpg">(digestion E-P)</a></td> | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9_10pg.jpg">(digestion E-P)</a></td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A9pg</td> | + | <tr class="in_freezer"><td class="prova">A9pg (BBa_K173009)</td> |
<td class="prova">A6-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td> | <td class="prova">A6-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td> | ||
<td class="prova">Jul 3</td> | <td class="prova">Jul 3</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9_10pg.jpg">(digestion E-P)</a></td> | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9_10pg.jpg">(digestion E-P)</a></td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A11-1<br> | + | <tr class="in_freezer"><td class="prova">A11-1<br>thrown away and replaced</td> |
<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td> | <td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td> | ||
<td class="prova">Jul 10</td> | <td class="prova">Jul 10</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A11.jpg">(PCR)</a><br>sequence MUTATED</td> | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A11.jpg">(PCR)</a><br>sequence MUTATED</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A11-3<br> | + | <tr class="in_freezer"><td class="prova">A11-3<br>thrown away and replaced</td> |
<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td> | <td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td> | ||
<td class="prova">Jul 10</td> | <td class="prova">Jul 10</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A11.jpg">(PCR)</a></td> | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A11.jpg">(PCR)</a></td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A11-6<br> | + | <tr class="in_freezer"><td class="prova">A11-6<br>thrown away and replaced</td> |
<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td> | <td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td> | ||
<td class="prova">Jul 10</td> | <td class="prova">Jul 10</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A11.jpg">(PCR)</a></td> | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A11.jpg">(PCR)</a></td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">F2620<br> | + | <tr class="in_freezer"><td class="prova">F2620<br>thrown away</td> |
<td class="prova">Ptet-RBS-luxR-TT-Plux<br></td> | <td class="prova">Ptet-RBS-luxR-TT-Plux<br></td> | ||
<td class="prova">Jul 10</td> | <td class="prova">Jul 10</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">sequence BAD (MIT, BMR)</td> | <td class="prova">sequence BAD (MIT, BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A12-2</td> | + | <tr class="in_freezer"><td class="prova">A12-2 (BBa_K173011)</td> |
<td class="prova">J23118(Jun 4)-A4<br></td> | <td class="prova">J23118(Jun 4)-A4<br></td> | ||
<td class="prova">Jul 16</td> | <td class="prova">Jul 16</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A12.jpg">(PCR)</a><br>sequence ok (BMR)</td> | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A12.jpg">(PCR)</a><br>sequence ok (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A12-3</td> | + | <tr class="in_freezer"><td class="prova">A12-3<br>it is a backup of A12-2</td> |
<td class="prova">J23118(Jun 4)-A4<br></td> | <td class="prova">J23118(Jun 4)-A4<br></td> | ||
<td class="prova">Jul 16</td> | <td class="prova">Jul 16</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A12.jpg">(PCR)</a><br>sequence ok (BMR)</td> | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A12.jpg">(PCR)</a><br>sequence ok (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A14-4<br> | + | <tr class="in_freezer"><td class="prova">A14-4<br>thrown away</td> |
<td class="prova">A11-E0240<br></td> | <td class="prova">A11-E0240<br></td> | ||
<td class="prova">Jul 16</td> | <td class="prova">Jul 16</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_digestion_A14.jpg">(digestion)</a> and the A11 sample used for ligation was MUTATED (BMR)</td> | <td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_digestion_A14.jpg">(digestion)</a> and the A11 sample used for ligation was MUTATED (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A14pg<br> | + | <tr class="in_freezer"><td class="prova">A14pg <br>thrown away</td> |
<td class="prova">A11-E0240<br></td> | <td class="prova">A11-E0240<br></td> | ||
<td class="prova">Jul 16</td> | <td class="prova">Jul 16</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a> and the A11 sample used for ligation was MUTATED (BMR)</td> | <td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a> and the A11 sample used for ligation was MUTATED (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">K116001 (PnhaA)</td> | ||
+ | <td class="prova">PnhaA-RBS-GFP-TT<br></td> | ||
+ | <td class="prova">Jul 29</td> | ||
+ | <td class="prova">NEB 10-beta</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">sequence WRONG! it is actually K116002!!!</td> | ||
</tr> | </tr> | ||
<tr class="in_freezer"><td class="prova">K116002 (PnhaA+GFP)</td> | <tr class="in_freezer"><td class="prova">K116002 (PnhaA+GFP)</td> | ||
<td class="prova">PnhaA-RBS-GFP-TT<br></td> | <td class="prova">PnhaA-RBS-GFP-TT<br></td> | ||
<td class="prova">Jul 29</td> | <td class="prova">Jul 29</td> | ||
- | <td class="prova"> | + | <td class="prova">NEB 10-beta</td> |
- | + | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
- | <td class="prova">sequence | + | <td class="prova">sequence WRONG! it is actually J33204!!!</td> |
</tr> | </tr> | ||
<tr class="in_freezer"><td class="prova">K112405 (Pcad)</td> | <tr class="in_freezer"><td class="prova">K112405 (Pcad)</td> | ||
<td class="prova">cadAB promoter<br></td> | <td class="prova">cadAB promoter<br></td> | ||
<td class="prova">Jul 29</td> | <td class="prova">Jul 29</td> | ||
- | <td class="prova"> | + | <td class="prova">NEB 10-beta</td> |
- | + | ||
<td class="prova">K112951(Amp+Cm)</td> | <td class="prova">K112951(Amp+Cm)</td> | ||
<td class="prova">sequence ok (BMR)</td> | <td class="prova">sequence ok (BMR)</td> | ||
Line 489: | Line 439: | ||
<td class="prova">RBS-lacI-TT<br></td> | <td class="prova">RBS-lacI-TT<br></td> | ||
<td class="prova">Jul 29</td> | <td class="prova">Jul 29</td> | ||
- | <td class="prova"> | + | <td class="prova">DH5alpha</td> |
- | + | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">sequence ok (BMR)</td> | <td class="prova">sequence ok (BMR)</td> | ||
Line 497: | Line 446: | ||
<td class="prova">GFPmut3+his tag generator under Pbad<br></td> | <td class="prova">GFPmut3+his tag generator under Pbad<br></td> | ||
<td class="prova">Jul 29</td> | <td class="prova">Jul 29</td> | ||
- | <td class="prova"> | + | <td class="prova">NEB 10-beta</td> |
- | + | ||
<td class="prova">pSB1AK3</td> | <td class="prova">pSB1AK3</td> | ||
<td class="prova">sequence ok -long part- (BMR)</td> | <td class="prova">sequence ok -long part- (BMR)</td> | ||
Line 505: | Line 453: | ||
<td class="prova">GFPmut3+his tag generator under PT7<br></td> | <td class="prova">GFPmut3+his tag generator under PT7<br></td> | ||
<td class="prova">Jul 29</td> | <td class="prova">Jul 29</td> | ||
- | <td class="prova"> | + | <td class="prova">NEB 10-beta</td> |
- | + | ||
<td class="prova">pSB1AK3</td> | <td class="prova">pSB1AK3</td> | ||
<td class="prova">sequence almost ok (BMR): the RBS is B0030 and not B0034 as documented, but the function is the same</td> | <td class="prova">sequence almost ok (BMR): the RBS is B0030 and not B0034 as documented, but the function is the same</td> | ||
Line 513: | Line 460: | ||
<td class="prova">MioC promoter<br></td> | <td class="prova">MioC promoter<br></td> | ||
<td class="prova">Jul 29</td> | <td class="prova">Jul 29</td> | ||
- | <td class="prova"> | + | <td class="prova">NEB 10-beta</td> |
- | + | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">sequence BAD (BMR)</td> | <td class="prova">sequence BAD (BMR)</td> | ||
Line 521: | Line 467: | ||
<td class="prova">Ptet-RBS-luxR-TT-Plux<br></td> | <td class="prova">Ptet-RBS-luxR-TT-Plux<br></td> | ||
<td class="prova">Jul 29</td> | <td class="prova">Jul 29</td> | ||
- | <td class="prova"> | + | <td class="prova">D1210(lacIq)</td> |
- | + | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">sequence ok (BMR)</td> | <td class="prova">sequence ok (BMR)</td> | ||
Line 529: | Line 474: | ||
<td class="prova">Ptet-RBS-luxR-TT-Plux<br></td> | <td class="prova">Ptet-RBS-luxR-TT-Plux<br></td> | ||
<td class="prova">Jul 29</td> | <td class="prova">Jul 29</td> | ||
- | <td class="prova"> | + | <td class="prova">D1210(lacIq)</td> |
- | + | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">sequence ok (BMR)</td> | <td class="prova">sequence ok (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">MRGENE1</td> | + | <tr class="in_freezer"><td class="prova">MRGENE1 (BBa_K173016)</td> |
<td class="prova">pdc</td> | <td class="prova">pdc</td> | ||
<td class="prova">Jul 17</td> | <td class="prova">Jul 17</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pMK-RQ<br>(Kan)</td> | <td class="prova">pMK-RQ<br>(Kan)</td> | ||
Line 545: | Line 488: | ||
<td class="prova">pdc</td> | <td class="prova">pdc</td> | ||
<td class="prova">Jul 17</td> | <td class="prova">Jul 17</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pMK-RQ<br>(Kan)</td> | <td class="prova">pMK-RQ<br>(Kan)</td> | ||
Line 553: | Line 495: | ||
<td class="prova">pdc</td> | <td class="prova">pdc</td> | ||
<td class="prova">Jul 17</td> | <td class="prova">Jul 17</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pMK-RQ<br>(Kan)</td> | <td class="prova">pMK-RQ<br>(Kan)</td> | ||
<td class="prova">antibiotic ok<br>sequence ok (Mr Gene)</td> | <td class="prova">antibiotic ok<br>sequence ok (Mr Gene)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">MRGENE2</td> | + | <tr class="in_freezer"><td class="prova">MRGENE2 (BBa_K173017)</td> |
<td class="prova">adhB</td> | <td class="prova">adhB</td> | ||
<td class="prova">Jul 17</td> | <td class="prova">Jul 17</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pMA<br>(Amp)</td> | <td class="prova">pMA<br>(Amp)</td> | ||
Line 569: | Line 509: | ||
<td class="prova">adhB</td> | <td class="prova">adhB</td> | ||
<td class="prova">Jul 17</td> | <td class="prova">Jul 17</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pMA<br>(Amp)</td> | <td class="prova">pMA<br>(Amp)</td> | ||
Line 577: | Line 516: | ||
<td class="prova">adhB</td> | <td class="prova">adhB</td> | ||
<td class="prova">Jul 17</td> | <td class="prova">Jul 17</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pMA<br>(Amp)</td> | <td class="prova">pMA<br>(Amp)</td> | ||
<td class="prova">antibiotic ok<br>sequence ok (Mr Gene)</td> | <td class="prova">antibiotic ok<br>sequence ok (Mr Gene)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">B1-13</td> | + | <tr class="in_freezer"><td class="prova">B1-13 (BBa_K173013)</td> |
<td class="prova">B0030-MRGENE1<br>(RBS-pdc)</td> | <td class="prova">B0030-MRGENE1<br>(RBS-pdc)</td> | ||
<td class="prova">Jul 28</td> | <td class="prova">Jul 28</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">length ok (<a href="https://2009.igem.org/Image:Pv_digestion_B1_B2.jpg">digestion E-P</a><br>, <a href="https://2009.igem.org/Image:Pv_digestion_B1_S-P.jpg">digestion S-P</a> and <a href="https://2009.igem.org/Image:Pv_B1E-S_B2E-S_B0015E-X_cut.jpg">digestion E-S</a>)<br>sequence ok (BMR)</td> | <td class="prova">length ok (<a href="https://2009.igem.org/Image:Pv_digestion_B1_B2.jpg">digestion E-P</a><br>, <a href="https://2009.igem.org/Image:Pv_digestion_B1_S-P.jpg">digestion S-P</a> and <a href="https://2009.igem.org/Image:Pv_B1E-S_B2E-S_B0015E-X_cut.jpg">digestion E-S</a>)<br>sequence ok (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">B2-5</td> | + | <tr class="in_freezer"><td class="prova">B2-5 (BBa_K173014)</td> |
<td class="prova">B0030-MRGENE2<br>(RBS-adhB)</td> | <td class="prova">B0030-MRGENE2<br>(RBS-adhB)</td> | ||
<td class="prova">Jul 23</td> | <td class="prova">Jul 23</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">length ok (<a href="https://2009.igem.org/Image:Pv_B2pcr_A14pgPstI.jpg">PCR</a><br> and <a href="https://2009.igem.org/Image:Pv_digestion_B1_B2.jpg">digestion S-P</a>)<br>sequence ok (BMR)</td> | <td class="prova">length ok (<a href="https://2009.igem.org/Image:Pv_B2pcr_A14pgPstI.jpg">PCR</a><br> and <a href="https://2009.igem.org/Image:Pv_digestion_B1_B2.jpg">digestion S-P</a>)<br>sequence ok (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">B3-5</td> | + | <tr class="in_freezer"><td class="prova">B3-5 (BBa_K173018)</td> |
<td class="prova">B1-B0015<br>(RBS-pdc-TT)</td> | <td class="prova">B1-B0015<br>(RBS-pdc-TT)</td> | ||
<td class="prova">Jul 31</td> | <td class="prova">Jul 31</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1AK3</td> | <td class="prova">pSB1AK3</td> | ||
<td class="prova">length ok+extra band (<a href="https://2009.igem.org/Image:Pv_B3_B4_digestion_PstI_XhoI.jpg">digestion XhoI-P</a><br> and <a href="https://2009.igem.org/Image:Pv_B3_digestion_X-P.jpg">digestion X-P</a>)<br>sequence ok (BMR)</td> | <td class="prova">length ok+extra band (<a href="https://2009.igem.org/Image:Pv_B3_B4_digestion_PstI_XhoI.jpg">digestion XhoI-P</a><br> and <a href="https://2009.igem.org/Image:Pv_B3_digestion_X-P.jpg">digestion X-P</a>)<br>sequence ok (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">B4-2</td> | + | <tr class="in_freezer"><td class="prova">B4-2 (BBa_K173019)</td> |
<td class="prova">B2-B0015<br>(RBS-adhB-TT)</td> | <td class="prova">B2-B0015<br>(RBS-adhB-TT)</td> | ||
<td class="prova">Jul 31</td> | <td class="prova">Jul 31</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1AK3</td> | <td class="prova">pSB1AK3</td> | ||
Line 617: | Line 551: | ||
<td class="prova">B1-B4<br>(RBS-pdc-RBS-adhB-TT)</td> | <td class="prova">B1-B4<br>(RBS-pdc-RBS-adhB-TT)</td> | ||
<td class="prova">Aug 12</td> | <td class="prova">Aug 12</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1AK3</td> | <td class="prova">pSB1AK3</td> | ||
<td class="prova">length ok+2 extra bands <a href="https://2009.igem.org/Image:Pv_digestion_B5_B6_E-P.jpg">(digestion E-P)</a><br>sequence ok (BMR)</td> | <td class="prova">length ok+2 extra bands <a href="https://2009.igem.org/Image:Pv_digestion_B5_B6_E-P.jpg">(digestion E-P)</a><br>sequence ok (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">B6-3</td> | + | <tr class="in_freezer"><td class="prova">B6-3 (BBa_K173020)</td> |
<td class="prova">B2-B3<br>(RBS-adhB-RBS-pdc-TT)</td> | <td class="prova">B2-B3<br>(RBS-adhB-RBS-pdc-TT)</td> | ||
<td class="prova">Aug 12</td> | <td class="prova">Aug 12</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1AK3</td> | <td class="prova">pSB1AK3</td> | ||
<td class="prova">length ok+2 extra bands <a href="https://2009.igem.org/Image:Pv_digestion_B5_B6_E-P.jpg">(digestion E-P)</a><br>sequence ok (BMR)</td> | <td class="prova">length ok+2 extra bands <a href="https://2009.igem.org/Image:Pv_digestion_B5_B6_E-P.jpg">(digestion E-P)</a><br>sequence ok (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A11-2<br> | + | <tr class="in_freezer"><td class="prova">A11-2<br>thrown away</td> |
<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td> | <td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td> | ||
<td class="prova">Aug 13</td> | <td class="prova">Aug 13</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion S-P)</a> sequence MUTATED (BMR)</td> | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion S-P)</a> sequence MUTATED (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A11-3</td> | + | <tr class="in_freezer"><td class="prova">A11-3 (BBa_K173010)</td> |
<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td> | <td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td> | ||
<td class="prova">Aug 13</td> | <td class="prova">Aug 13</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion S-P)</a> sequence ok (BMR)</td> | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion S-P)</a> sequence ok (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A15-1<br> | + | <tr class="in_freezer"><td class="prova">A15-1<br>it is a backup copy of A15-3</td> |
<td class="prova">F2620MIT1-K112808<br>(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)</td> | <td class="prova">F2620MIT1-K112808<br>(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)</td> | ||
<td class="prova">Aug 14</td> | <td class="prova">Aug 14</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a> sequence ok (BMR)</td> | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a> sequence ok (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A15-2</td> | + | <tr class="in_freezer"><td class="prova">A15-2<br>thrown away</td> |
<td class="prova">F2620MIT1-K112808<br>(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)</td> | <td class="prova">F2620MIT1-K112808<br>(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)</td> | ||
<td class="prova">Aug 14</td> | <td class="prova">Aug 14</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a> sequence WRONG (BMR)</td> | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a> sequence WRONG (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A15-3</td> | + | <tr class="in_freezer"><td class="prova">A15-3 (BBa_K173015)</td> |
<td class="prova">F2620MIT1-K112808<br>(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)</td> | <td class="prova">F2620MIT1-K112808<br>(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)</td> | ||
<td class="prova">Aug 14</td> | <td class="prova">Aug 14</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a> sequence ok (BMR)</td> | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a> sequence ok (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">B8-5<br>thrown away | + | <tr class="in_freezer"><td class="prova">B8-5<br>thrown away</td> |
<td class="prova">A4-B6<br>(RBS-tetR-TT-Ptet-RBS-adhB-RBS-pdc-TT)</td> | <td class="prova">A4-B6<br>(RBS-tetR-TT-Ptet-RBS-adhB-RBS-pdc-TT)</td> | ||
<td class="prova">Aug 21</td> | <td class="prova">Aug 21</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1AK3</td> | <td class="prova">pSB1AK3</td> | ||
- | <td class="prova">length | + | <td class="prova">length ??? (digestion E-P) sequence WRONG (BMR)</td> |
</tr> | </tr> | ||
<tr class="in_freezer"><td class="prova">A14-3<br>to throw away!</td> | <tr class="in_freezer"><td class="prova">A14-3<br>to throw away!</td> | ||
<td class="prova">A11(-2 Aug13)-E0240<br>(Pcon-RBS-lacI-TT-Plac-RBS-GFP-TT)</td> | <td class="prova">A11(-2 Aug13)-E0240<br>(Pcon-RBS-lacI-TT-Plac-RBS-GFP-TT)</td> | ||
<td class="prova">Aug 21</td> | <td class="prova">Aug 21</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(PCR)</a> sequence of A11-2(Aug13) was MUTATED (BMR)</td> | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(PCR)</a> sequence of A11-2(Aug13) was MUTATED (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A16-4</td> | + | <tr class="in_freezer"><td class="prova">A16-4 (BBa_K173026)</td> |
<td class="prova">A12-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td> | <td class="prova">A12-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td> | ||
<td class="prova">Aug 21</td> | <td class="prova">Aug 21</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 697: | Line 621: | ||
<td class="prova">R0011-E0240<br>(Plac-RBS-GFP-TT)</td> | <td class="prova">R0011-E0240<br>(Plac-RBS-GFP-TT)</td> | ||
<td class="prova">Aug 28</td> | <td class="prova">Aug 28</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(PCR)</a> sequence WRONG! ligation failed.</td> | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(PCR)</a> sequence WRONG! ligation failed.</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A18 | + | <tr class="in_freezer"><td class="prova">A18<br>thrown away</td> |
<td class="prova">A15(-2)-pSB3K3<br>(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)</td> | <td class="prova">A15(-2)-pSB3K3<br>(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)</td> | ||
<td class="prova">Aug 28</td> | <td class="prova">Aug 28</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB3K3</td> | <td class="prova">pSB3K3</td> | ||
- | <td class="prova"> | + | <td class="prova">not screened, its parent plasmid (A15-2) was wrong</td> |
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">K116002 (PnhaA+GFP)</td> | ||
+ | <td class="prova">PnhaA-RBS-GFP-TT<br></td> | ||
+ | <td class="prova">Sep 4</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">sequence almost ok (BMR): inconsistent prefix (but restriction sites are good) and additional "c" at the end of promoter</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">B5new2-3 (BBa_K173003)</td> | ||
+ | <td class="prova">B1-B4 (RBS-pdc-RBS-adhB-TT)</td> | ||
+ | <td class="prova">Sep 1</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1AK3</td> | ||
+ | <td class="prova">length ok (digestion E-P), sequence ok -long part- (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">B9-2 (BBa_K173021)</td> | ||
+ | <td class="prova">F2620-B5new2(-3) (Ptet-RBS-luxR-TT-Plux-RBS-pdc-RBS-adhB-TT)</td> | ||
+ | <td class="prova">Sep 4</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1AK3</td> | ||
+ | <td class="prova">length ok (digestion E-P), sequence ok -long part- (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">B10-2 (BBa_K173022)</td> | ||
+ | <td class="prova">F2620-B6 (Ptet-RBS-luxR-TT-Plux-RBS-adhB-RBS-pdc-TT)</td> | ||
+ | <td class="prova">Sep 4</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1AK3</td> | ||
+ | <td class="prova">length ok + 2 extra bands (digestion E-P), sequence ok -long part- (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">B9new-1 to 5</td> | ||
+ | <td class="prova">F2620-B5new2(-3) (Ptet-RBS-luxR-TT-Plux-RBS-pdc-RBS-adhB-TT)</td> | ||
+ | <td class="prova">Oct 1</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1AK3</td> | ||
+ | <td class="prova">length ok (digestion E-P)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A19</td> | ||
+ | <td class="prova">F2620</td> | ||
+ | <td class="prova">Oct 1</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB4C5</td> | ||
+ | <td class="prova">length ok (digestion E-P)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A21</td> | ||
+ | <td class="prova">A19-B5new2(-3)</td> | ||
+ | <td class="prova">Oct 8</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB4C5</td> | ||
+ | <td class="prova">length ok (digestion E-P)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A14S-3 (BBa_K173012)</td> | ||
+ | <td class="prova">J23118-RBS-lacI-TT-Plac-RBS-GFP-TT (A11(-3)-E0240)</td> | ||
+ | <td class="prova">?</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">length ok (digestion E-P)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A17new (BBa_K173025)</td> | ||
+ | <td class="prova">Plac-RBS-GFP-TT (R0011-E0240)</td> | ||
+ | <td class="prova">Sep 4</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">length ok (PCR), sequence ok (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A20</td> | ||
+ | <td class="prova">F2620-A8 (Ptet-RBS-luxR-TT-Plux-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td> | ||
+ | <td class="prova">Oct 8</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">length ok (digestion E-P)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">I52002 (4C5)</td> | ||
+ | <td class="prova">plasmid</td> | ||
+ | <td class="prova">?</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB4C5</td> | ||
+ | <td class="prova">length ok (digestion E-P)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">I714891 (3K3)</td> | ||
+ | <td class="prova">plasmid</td> | ||
+ | <td class="prova">Aug 21</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB3K3</td> | ||
+ | <td class="prova">length ok (digestion E-P)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">B14 (BBa_K173023)</td> | ||
+ | <td class="prova">F2620-B3 (Ptet-RBS-luxR-TT-Plux-RBS-pdc-TT)</td> | ||
+ | <td class="prova">Oct 16</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">to screen</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">B15 (BBa_K173024)</td> | ||
+ | <td class="prova">F2620-B4 (Ptet-RBS-luxR-TT-Plux-RBS-adhB-TT)</td> | ||
+ | <td class="prova">Oct 16</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">to screen</td> | ||
</tr> | </tr> | ||
Latest revision as of 12:32, 21 October 2009
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