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Team:KULeuven/Design/Blue Light Receptor
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| - | + | = Blue Light Receptor: Design = | |
| - | + | [[Image:Biologie blue light promotor.png|center]] | |
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{{kulpart|BBa_K238013}} | {{kulpart|BBa_K238013}} | ||
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| - | The purpose of the receptor is to enter the wanted vanillin concentration. The protein YcgF is a known blue-light sensor in certain E. coli strains. Upon photo-excitation it dimerizes and acts as an anti-repressor for YcgE. YcgE is bound to the promotor-region and inhibits RNA Polymerase. The dimerized YcgF interacts directly with the repressor, releasing it from the DNA and allowing transcription. The light used to perform the test has a wavelength of 470nm. | + | The purpose of the receptor is to enter the wanted vanillin concentration. The protein YcgF is a known blue-light sensor in certain ''E. coli'' strains. Upon photo-excitation it dimerizes and acts as an anti-repressor for YcgE. YcgE is bound to the promotor-region of the ''key''-gene and inhibits RNA Polymerase. The dimerized YcgF interacts directly with the repressor, releasing it from the DNA and allowing transcription. The light used to perform the test has a wavelength of 470nm. |
| - | We designed the {{kulpart|BBa_K238002}} part in such way that irradiation with a certain amount of blue light activates transcription of RIBOKEY-mRNA. To achieve this, we purified the promoter-region of the E. coli- strain MC4100. | + | We designed the {{kulpart|BBa_K238002}} part in such way that irradiation with a certain amount of blue light activates transcription of RIBOKEY-mRNA. To achieve this, we purified the promoter-region of the ''E. coli''- strain MC4100. The ''blr'' promoter part ({{kulpart|BBa_K238013}}) was added to the registry. |
{{Team:KULeuven/Common2/PageFooter}} | {{Team:KULeuven/Common2/PageFooter}} | ||
Latest revision as of 16:09, 20 October 2009
Blue Light Receptor: Design
The purpose of the receptor is to enter the wanted vanillin concentration. The protein YcgF is a known blue-light sensor in certain E. coli strains. Upon photo-excitation it dimerizes and acts as an anti-repressor for YcgE. YcgE is bound to the promotor-region of the key-gene and inhibits RNA Polymerase. The dimerized YcgF interacts directly with the repressor, releasing it from the DNA and allowing transcription. The light used to perform the test has a wavelength of 470nm.
We designed the part in such way that irradiation with a certain amount of blue light activates transcription of RIBOKEY-mRNA. To achieve this, we purified the promoter-region of the E. coli- strain MC4100. The blr promoter part () was added to the registry.
