Template:Team:KULeuven/4 September 2009/VanillinProduction

From 2009.igem.org

(Difference between revisions)
 
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* The restriction digest from SAMS and the terminator was put on gel
* The restriction digest from SAMS and the terminator was put on gel
-
** It appears there is no SAMS in between the vector, the length is approx. 1500 bp which can only be Sam5 or Sam8
+
** It appears there is no SAMS in between the vector, the length is approx. 1500 bp which can only be ''sam5'' or'' sam8''
* Electroporation of the ligation EF
* Electroporation of the ligation EF
-
** Plated and put on 37C
+
** Plated and put at 37°C
-
* Liquid cultures made from sam5, sam8, ech and fcs.
+
* Liquid cultures made from ''sam5, sam8, ech'' and ''fcs''.

Latest revision as of 12:02, 14 September 2009

  • The restriction digest from SAMS and the terminator was put on gel
    • It appears there is no SAMS in between the vector, the length is approx. 1500 bp which can only be sam5 or sam8
  • Electroporation of the ligation EF
    • Plated and put at 37°C
  • Liquid cultures made from sam5, sam8, ech and fcs.