Team:Chiba/Notebook/Calendar/12 September 2009

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== Check of Sender's AHL generation (2) ==
== Check of Sender's AHL generation (2) ==
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===08:15 Main culture===
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*Main culture
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===Measuring OD===
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8:15-
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*13:35 OD<sub>600</sub>=1.02
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*14:45 OD<sub>600</sub>=1.42
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*16:20 OD<sub>600</sub>=1.64
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*Measuring OD
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*18:30 OD<sub>600</sub>=1.72
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13:35
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-
 
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OD<sub>600</sub>=1.02
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-
 
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We cultured again.
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-
 
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-
 
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14:45
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-
 
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OD<sub>600</sub>=1.42
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-
 
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We cultured again.
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-
 
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-
 
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16:20
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-
 
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OD<sub>600</sub>=1.64
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-
 
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We cultured again.
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-
 
+
-
 
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18:30
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-
 
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OD<sub>600</sub>=1.72
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-
 
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We stopped culture.
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'''We stopped culture and the experiments.'''
== Check of Sender's AHL generation (3) ==
== Check of Sender's AHL generation (3) ==
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=== 19:00 Prior culture ===
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*Prior culture
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19:00
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We did prior culture using glycerol stock(XL10G, plac-LuxI, +LVA) .
We did prior culture using glycerol stock(XL10G, plac-LuxI, +LVA) .
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== ''E.'' coli Painting ==
== ''E.'' coli Painting ==
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Image:Chiba_1109-4.JPG
Image:Chiba_1109-4.JPG
</gallery>
</gallery>
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== Handwritten Lab Notebook ==
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[[Image:Chiba-Labwork-11Sep09.jpg|200px]] [[Image:Chiba-Labwork-12Sep09.jpg|200px]]

Latest revision as of 18:29, 23 September 2009

>Go to the Notebook page

(11_September_2009 <|>13_September_2009)


Contents

Check of Sender's AHL generation (2)

08:15 Main culture

Measuring OD

  • 13:35 OD600=1.02
  • 14:45 OD600=1.42
  • 16:20 OD600=1.64
  • 18:30 OD600=1.72

We stopped culture and the experiments.

Check of Sender's AHL generation (3)

19:00 Prior culture

We did prior culture using glycerol stock(XL10G, plac-LuxI, +LVA) .

E. coli Painting

Chiba 1109-1.JPG
Chiba 1109-2.JPG
Chiba 1109-3.JPG
Chiba 1109-4.JPG


Handwritten Lab Notebook

Chiba-Labwork-11Sep09.jpg Chiba-Labwork-12Sep09.jpg

Retrieved from "http://2009.igem.org/Team:Chiba/Notebook/Calendar/12_September_2009"