Team:British Columbia

From 2009.igem.org

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=<font color="#00CC66">E.coli</font> <font color="#FFCC66">Traffic</font> <font color="#FF0000">Light</font>: <br> A ''flexible'', ''modular'', and ''transparent'' system for multi-level assessment of variable inputs.'=
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Biosensors have a diverse variety of real-world functions, ranging from measuring blood glucose levels in diabetes patients to assessing environmental contamination of trace toxins. The majority of these sensors are highly specific for a single input, and their outputs often require specialized equipment such as surface plasmon resonance chips.
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Our project aims to create a biosensor that recognizes a specific target and alters its output fluorescence from green, to yellow, to red as a function of concentration up to critical levels (hence, a biological "traffic light").
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Click the colours of the traffic light to learn about its different subparts!
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
 
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook.  PLEASE keep all of your pages within your teams namespace. 
 
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<img src="/wiki/images/thumb/f/fc/E_coli_Traffic_Light_General.png/950px-E_coli_Traffic_Light_General.png" width=950 usemap="#trafficlight">
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|Formed in March, 2009, the UBC iGEM team was destined for greatness.
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      alt="Sensors: This links to the pBAD promoters that sense the arabinose."
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      title="Sensors: This links to the pBAD promoters that sense the arabinose."
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''We have selected two project tracks:
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      href="/Team:British_Columbia/pBAD"
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  * To manipulate the sensitivity of a whole-cell biosensor
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  * To implement a logic gate-based decision system within the biosensor
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The general scheme is to prototype the manipulations using an arabinose sensor coupled to a GFP reporter. ''
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      alt="Lock and Key: this controls when the colors are produced"
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!align="center"|[[Team:British_Columbia|Home]]
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!align="center"|[[Team:British_Columbia/Team|The Team]]
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      alt="The Jammer: this mRNA sequence blocks the key from opening the lock."
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!align="center"|[[Team:British_Columbia/Project|The Project]]
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      title="The Jammer: this mRNA sequence blocks the key from opening the lock."
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!align="center"|[[Team:British_Columbia/Parts|Parts Submitted to the Registry]]
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      href="/Team:British_Columbia/Jammer"
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!align="center"|[[Team:British_Columbia/Modeling|Modeling]]
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!align="center"|[[Team:British_Columbia/Biosensor_Sensitivity|Biosensor Sensitivity Notebook]]
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!align="center"|[[Team:British_Columbia/Biosensor_Logic_Gate|Biosensor Logic Gate Notebook]]
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[[Image:E_coli_Traffic_Light_General.png|thumb|center|950px|Schematic black-box representation of the E. coli Biosensor that detects various concentration inputs and color outputs. The idea is discrete analog outputs based on a user-specified threshold for each range of concentration.]]
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Latest revision as of 03:58, 22 October 2009

E.coli Traffic Light:
A flexible, modular, and transparent system for multi-level assessment of variable inputs.'

Biosensors have a diverse variety of real-world functions, ranging from measuring blood glucose levels in diabetes patients to assessing environmental contamination of trace toxins. The majority of these sensors are highly specific for a single input, and their outputs often require specialized equipment such as surface plasmon resonance chips.

Our project aims to create a biosensor that recognizes a specific target and alters its output fluorescence from green, to yellow, to red as a function of concentration up to critical levels (hence, a biological "traffic light").


Click the colours of the traffic light to learn about its different subparts!

Sensors: This links to the pBAD promoters that sense the arabinose. Lock and Key: this controls when the colors are produced The Jammer: this mRNA sequence blocks the key from opening the lock.



Retrieved from "http://2009.igem.org/Team:British_Columbia"