Team:Wash U/Calendar-Home/10 June 2009

(Difference between revisions)

Latest revision as of 20:13, 24 June 2009

Altering Transformation Protocol to adhere to iGem Help Page

Estimated 5-15ng of DNA per well.

Using 5ul of DNA (diluted 1:16 in TE)

Allow the DNA and competent cells to sit on ice for 30 minutes

Heat shock at 42ºC for 60 sec in water bath.

Recover on ice for 5 min.

Incubate at 37ºC for 2 hr while the tubes are rotating.

Plate 250µL on an LB plate with the appropriate antibiotic.

-Team Meeting #2

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-==Altering Transformation Protocol to adhere to iGem Help Page==
+{{WashUback}}
+== Wednesday June 10, 2009 ==
+'''Altering Transformation Protocol to adhere to iGem Help Page'''
 Estimated 5-15ng of DNA per well.
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-[[Team:Wash_U/Notebook|Back to Calendar]]
+-Team Meeting #2
+ <div style="text-align: right;">
+<div style="float: left;">
+[[Team:Wash_U/Calendar-Home/9 June 2009|Previous entry]]</div>
+ [[Team:Wash_U/Calendar-Home/11 June 2009|Next entry]]</div>
+<div style="clear: left;"></div>
+<center>[[Team:Wash U/Notebook|Back to Calendar]]</center>
+{{WashUbottom}}