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Team:Warsaw/Calendar-Main/28 May 2009
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(New page: {{WarNotebook}} <!-- do not edit above me! --> <html> <h3>digestion of constructs pKS/hly</h3> <h4><font color="red">Marcin?</font></h4> <img src="https://static.igem.org/mediawiki/2009/1/11/20...) |
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| - | <h3> | + | <h3>Cloning of hly gene into pKSII+ vector</h3> |
| - | <h4>< | + | <h4>Marcin</h4> |
| - | <img src="https://static.igem.org/mediawiki/2009/1/11/2009.05.28_-_trawienia_pKS%2Bhly_przyciety.jpg" | + | <br/> |
| + | <ul> | ||
| + | <li>Task:</li> | ||
| + | </ul> | ||
| + | <p>Restriction digest of isolated plasmids pKS/hly to confirm the correctness of the ligation</p> | ||
| + | <p>Methods:</p> | ||
| + | <ul> | ||
| + | <li>Composition of reaction mixture: | ||
| + | <pre> | ||
| + | 1 μl purified plasmid solution | ||
| + | 0.5 μl HindIII (Fermentas) | ||
| + | 2 μl Buffer Red (Fermentas) | ||
| + | 15.5 μl MQ water</pre> | ||
| + | </li> | ||
| + | <li>Digest</li> | ||
| + | </ul> | ||
| + | <br/> | ||
| + | <p>Program:</p> | ||
| + | <p>description of the program is <a href= "https://2009.igem.org/Team:Warsaw/Calendar-Main/13_July_2009">here</a></p> | ||
| + | <p>After digest samples were loaded into gel and electrophoretically separated</p> | ||
| + | <br/> | ||
| + | <p>Electrophoresis conditions:</p> | ||
| + | <p>voltage - 100V</p> | ||
| + | <p>time: 40 minutes</p> | ||
| + | <br/> | ||
| + | <img src="https://static.igem.org/mediawiki/2009/1/11/2009.05.28_-_trawienia_pKS%2Bhly_przyciety.jpg" height="30%" width="30%"> | ||
| + | <br/> | ||
| + | Fig.1. Restriction pattern of analysed samples | ||
| + | <br/> | ||
| + | <p><strong>Comment:</strong></p> | ||
| + | |||
| + | <p>Sample 1 and 2 have expected resctricion pattern.</p> | ||
| + | |||
</html> | </html> | ||
| + | |||
Latest revision as of 17:48, 17 September 2009
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Cloning of hly gene into pKSII+ vector
Marcin
- Task:
Restriction digest of isolated plasmids pKS/hly to confirm the correctness of the ligation
Methods:
- Composition of reaction mixture:
1 μl purified plasmid solution 0.5 μl HindIII (Fermentas) 2 μl Buffer Red (Fermentas) 15.5 μl MQ water
- Digest
Program:
description of the program is here
After digest samples were loaded into gel and electrophoretically separated
Electrophoresis conditions:
voltage - 100V
time: 40 minutes
Fig.1. Restriction pattern of analysed samples
Comment:
Sample 1 and 2 have expected resctricion pattern.
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