Team:IPN-UNAM-Mexico/Notebook

From 2009.igem.org

(Difference between revisions)
 
(36 intermediate revisions not shown)
Line 1: Line 1:
-
<div align="center">
+
{{Template:IPN-UNAM-Mexico}}
-
{|
+
 
-
|| || valign="top" |
+
=NOTEBOOK=
-
<!-- {{{Año}}}/06 (Junio) -->
+
 
-
{| 
+
 
-
| colspan="7" style="background:#23238E;" | <Center>[[Team:IPN-UNAM-Mexico/Notebook/June|<font color="#D9D9F3">June</font>]]</Center>
+
-
|- bgcolor="#BCD2EE"
+
As summer proyect our experimental work began at july the 7th 2009 using 2008 bioparts catalog, unfortunately it didn't work properly, so we had a lot of trouble to take out DNA and transform into ''E. coli''. For this reason we had to delay lab work and request the 2009 catalog.
-
| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font>
+
 
 +
<h1>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Protocols|Protocols]]</h1>
 +
 
 +
<h2>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Notebook/July|July]]</h2>
 +
<h2>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Notebook/August|August]]</h2>
 +
<h2>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Notebook/September|September]]</h2>
 +
<h2>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Notebook/October|October]]</h2>
 +
 
 +
<h1>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Safety|Safety]]</h1>
 +
Our synthetic construction consists of 29 bioparts, which needed 12 ligations and different strategies to make it functional as is  described below:
 +
 
 +
 
 +
<center>
 +
{| border="2"
 +
!Number
 +
!Biobrick
 +
!Restriction
|-
|-
-
| &nbsp;1||&nbsp;2 || &nbsp;3 || &nbsp;&nbsp;4 || &nbsp;5 || &nbsp;6 || &nbsp;7 ||
+
|2
 +
|BBa_R0079
 +
|S & P
|-
|-
-
| &nbsp;8 ||&nbsp;9 || 10 || &nbsp;11 || 12 || 13 || 14 ||
+
|3
 +
|BBa_F1610
 +
|X & P
|-
|-
-
| 15||16 || 17 || &nbsp;18 || 19 || 20 || 21 ||
+
|4
 +
|BBa_K091146
 +
|S & P
|-
|-
-
| 22||23 || 24 || &nbsp;25 || 26 || 27 || 28 ||
+
|5
 +
|BBa_K093005
 +
|X &  
|-
|-
-
| 29||30 || &nbsp;<Font Color="#c0c0c0">1</Font> || &nbsp;&nbsp;<Font Color="#c0c0c0">2</Font> || &nbsp;<Font Color="#c0c0c0">3</Font> || &nbsp;<Font Color="#c0c0c0">4</Font> || &nbsp;<Font Color="#c0c0c0">5</Font>
+
|6
-
|}
+
|BBa_K081016
-
|| || valign="top" |
+
|X & P
-
<!-- {{{Año}}}/07 (Julio) -->
+
-
{| 
+
-
| colspan="7" style="background:#23238E;" | <Center>[[Team:IPN-UNAM-Mexico/Notebook/July|<font color="#D9D9F3">July</font>]]</Center>
+
-
|- bgcolor="#BCD2EE"
+
-
| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font>
+
|-
|-
-
| <Font Color="#c0c0c0">29</Font> || <Font Color="#c0c0c0">30</Font> || &nbsp;1 || &nbsp;2 || &nbsp;3 || &nbsp;4 || &nbsp;5 ||
+
|7
 +
|BBa_EC840
 +
|X & P
|-
|-
-
| &nbsp;6||&nbsp;[[Team:IPN-UNAM-Mexico/Notebook/July_7_2009#July 7th|7]] || &nbsp;[[Team:IPN-UNAM-Mexico/Notebook/July_8_2009#July 8th|8]] || &nbsp;&nbsp;[[Team:IPN-UNAM-Mexico/Notebook/July_9_2009#July 9th|9]] || [[Team:IPN-UNAM-Mexico/Notebook/July_10_2009#July 10th|10]] || [[Team:IPN-UNAM-Mexico/Notebook/July_11_2009#July 11th|11]] || 12 ||
+
|8
 +
|BBa_K081009
 +
|X & P
|-
|-
-
| 13 ||[[Team:IPN-UNAM-Mexico/Notebook/July#July 14th|14]] || [[Team:IPN-UNAM-Mexico/Notebook/July#July 15th|15]] || &nbsp;[[Team:IPN-UNAM-Mexico/Notebook/July#July 16th|16]] || [[Team:IPN-UNAM-Mexico/Notebook/July#July 17th|17]] || [[Team:IPN-UNAM-Mexico/Notebook/July#July 18th|18]] || 19 ||
+
|9
 +
|BBa_R0051
 +
|S &P
|-
|-
-
| 20||[[Team:IPN-UNAM-Mexico/Notebook/July#July 21st|21]] || [[Team:IPN-UNAM-Mexico/Notebook/July#July 22nd|22]] || &nbsp;[[Team:IPN-UNAM-Mexico/Notebook/July#July 23rd|23]] || [[Team:IPN-UNAM-Mexico/Notebook/July#July 24th|24]] || [[Team:IPN-UNAM-Mexico/Notebook/July#July 25th|25]] || [[Team:IPN-UNAM-Mexico/Notebook/July#July 26th|26]] ||
+
|10
 +
|BBa_J06800
 +
|X & P
|-
|-
-
| 27||[[Team:IPN-UNAM-Mexico/Notebook/July#July 28th|28]]|| [[Team:IPN-UNAM-Mexico/Notebook/July#July 29th|29]] || &nbsp;[[Team:IPN-UNAM-Mexico/Notebook/July#July 30th|30]] || [[Team:IPN-UNAM-Mexico/Notebook/July#July 31st|31]] || <Font Color="#c0c0c0">&nbsp;&nbsp;1</Font> || <Font Color="#c0c0c0">&nbsp;2</Font>
+
|11
-
|}
+
|BBa_Q04121
-
|}
+
|X & P
-
{|
+
-
|| || valign="top" |
+
-
<!-- {{{Año}}}/08 (Agosto) -->
+
-
{| 
+
-
| colspan="7" style="background:#23238E;" | <Center>[[Team:IPN-UNAM-Mexico/Notebook/August|<font color="#D9D9F3">August</font>]]</Center>
+
-
|- bgcolor="#BCD2EE"
+
-
| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font>
+
|-
|-
-
| <Font Color="#c0c0c0">27</Font> || <Font Color="#c0c0c0">28</Font> || <Font Color="#c0c0c0">29</Font> || &nbsp;<Font Color="#c0c0c0">30</Font> || <Font Color="#c0c0c0">31</Font> || &nbsp;[[Team:IPN-UNAM-Mexico/Notebook/August #August 1st|1]] || &nbsp;2 
+
|12
 +
|BBa_B0034
 +
|S & P
|-
|-
-
| &nbsp;3 ||&nbsp;4 || &nbsp;[[Team:IPN-UNAM-Mexico/Notebook/August#August 5th|5]] || &nbsp;&nbsp;[[Team:IPN-UNAM-Mexico/Notebook/August#August 6th|6]] || &nbsp;[[Team:IPN-UNAM-Mexico/Notebook/August#August 7th|7]] || &nbsp;[[Team:IPN-UNAM-Mexico/Notebook/August#August 8th|8]] || &nbsp;[[Team:IPN-UNAM-Mexico/Notebook/August#August 9th|9]]
+
|13
 +
|BBa_C0079
 +
|E & S
|-
|-
-
| 10 || [[Team:IPN-UNAM-Mexico/Notebook/August#August 11th|11]] || [[Team:IPN-UNAM-Mexico/Notebook/August#August 12th|12]] || &nbsp;[[Team:IPN-UNAM-Mexico/Notebook/August#August 13th|13]]|| [[Team:IPN-UNAM-Mexico/Notebook/August#August 14th|14]]|| [[Team:IPN-UNAM-Mexico/Notebook/August#August 15th|15]] || 16
+
|14
 +
|BBa_C0179
 +
|E & S
|-
|-
-
|17|| [[Team:IPN-UNAM-Mexico/Notebook/August#August 18th|18]] || 19 || &nbsp;20 || [[Team:IPN-UNAM-Mexico/Notebook/August#August 21st|21]] || 22 || [[Team:IPN-UNAM-Mexico/Notebook/August#August 23rd|23]]
+
|15
 +
|BBa_B0015
 +
|E & X
|-
|-
-
| 24||[[Team:IPN-UNAM-Mexico/Notebook/August#August 25th|25]] || 26 || &nbsp;[[Team:IPN-UNAM-Mexico/Notebook/August#August 27th | 27]] || 28 || [[Team:IPN-UNAM-Mexico/Notebook/August#August 29th | 29 ]] || [[Team:IPN-UNAM-Mexico/Notebook/August#August 30th | 30]]
+
|16
 +
|BBa_K081018
 +
|E & S
|-
|-
-
|31||<Font Color="#c0c0c0">1</Font> || <Font Color="#c0c0c0">2</Font> || <Font Color="#c0c0c0">3</Font> || <Font Color="#c0c0c0">4</Font> || <Font Color="#c0c0c0">5</Font> || <Font Color="#c0c0c0">6</Font>
+
|17
-
|}
+
|BBa_K116640
-
|| || valign="top" |
+
|X & P
-
<!-- {{{Año}}}/09 (Septiembre) -->
+
-
{|
+
-
| colspan="7" style="background:#23238E;" | <Center>[[Team:IPN-UNAM-Mexico/Notebook/September|<font color="#D9D9F3">September</font>]]</Center>
+
-
|- bgcolor="#BCD2EE"
+
-
| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font>
+
|-
|-
-
| <Font Color="#c0c0c0">31</Font>||&nbsp;1 || &nbsp;2 || &nbsp;&nbsp;3 || &nbsp;4 || &nbsp;5 || &nbsp;6||
+
|23
 +
|BBa_S03154
 +
|X & P
|-
|-
-
| &nbsp;7||&nbsp;8 || &nbsp;9 || &nbsp;10 || 11 || 12 || 13 ||  
+
|24
 +
|BBa_k145201
 +
|E & S
 +
|}
 +
</center>
 +
 
 +
 
 +
<center>
 +
{| border="2"
 +
! Ligation
 +
! Nickname
 +
! Biobrick
|-
|-
-
| 14 ||15 || 16 || &nbsp;17 || 18 || 19 || 20 ||  
+
|4-23
 +
|Ciencias I
 +
|BBa_K226005
|-
|-
-
| 21||22 || 23 || &nbsp;24 || 25 || 26 || 27 ||  
+
|13-15
 +
|Ciencias II
 +
|BBa_K266002
|-
|-
-
| 28||29 || 30 || &nbsp;<Font Color="#c0c0c0">1</Font> || <Font Color="#c0c0c0">2</Font> || <Font Color="#c0c0c0">3</Font> || <Font Color="#c0c0c0">4</Font>
+
|Ciencias I-7
-
|}
+
|Ciencias III
-
|| || valign="top" |
+
|BBa_K266006
-
<!-- {{{Año}}}/10 (Octubre) -->
+
-
{| 
+
-
| colspan="7" style="background:#23238E;" | <Center>[[Team:IPN-UNAM-Mexico/Notebook/October|<font color="#D9D9F3">October</font>]]</Center>
+
-
|- bgcolor="#BCD2EE"
+
-
| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font>
+
|-
|-
-
| <Font Color="#c0c0c0">28</Font> ||<Font Color="#c0c0c0">29</Font> || <Font Color="#c0c0c0">30</Font> || &nbsp;&nbsp; 1|| &nbsp;2 || &nbsp;3 || &nbsp;4
+
|24-17
 +
|Ciencias IV
 +
|BBa_K266001
|-
|-
-
| &nbsp;5||&nbsp;6 || &nbsp;7  || &nbsp;&nbsp;8|| 9 || 10 || 11||  
+
|2-3
 +
|Ciencias V
 +
|BBa_K266000
|-
|-
-
| 12||13 || 14  || &nbsp;15 || 16 || 17 || 18 ||  
+
|J231000-11
 +
|Ciencias VI
 +
|BBa_K266008
|-
|-
-
| 19||20 || 21 || &nbsp;22 || 23  || 24  || 25 ||  
+
|Amplicon - Ciencias II
 +
|Ciencias VII
 +
|BBa_K266003
|-
|-
-
| 26|| 27  || 28 || &nbsp;29 || 30 || 31 || <Font Color="#c0c0c0">&nbsp;1</Font>
+
|Ciencias VI - 12
-
|}
+
|Ciencias VIII
-
|| || valign="top" |
+
|BBa_K266009
-
<!-- {{{Año}}}/11 (Noviembre) -->
+
-
{|  
+
-
| colspan="7" style="background:#23238E;" | <Center>[[Team:IPN-UNAM-Mexico/Notebook/November|<font color="#D9D9F3">November</font>]]</Center>
+
-
|- bgcolor="#BCD2EE"
+
-
| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font>
+
|-
|-
-
| <Font Color="#c0c0c0">26</Font> ||<Font Color="#c0c0c0">27</Font> || <Font Color="#c0c0c0">28</Font> || <Font Color="#c0c0c0">&nbsp;29</Font> || <Font Color="#c0c0c0">30</Font> || <Font Color="#c0c0c0">31</Font> || &nbsp;1
+
|Ciencias III - 19
 +
|Ciencias IX
 +
|BBa_K266006
|-
|-
-
| &nbsp;2||&nbsp;3 || &nbsp;4 || &nbsp;&nbsp;5 || &nbsp;6 || &nbsp;7|| &nbsp;8
+
|J23100 – Ciencias VII
 +
|Ciencias X
 +
|BBa_K266004
|-
|-
-
| &nbsp;9||10 || 11 || &nbsp;12 || 13 || 14 || 15 ||  
+
|Ciencias III - Ciencias V
 +
|Ciencias XI
 +
|BBa_K266007
|-
|-
-
| 16||17 || 18 || &nbsp;19 || 20 || 21 || 22 ||
+
|Ciencias X - Ciencias IV
-
|-
+
|Ciencias XII
-
| 23 ||24 || 25 || &nbsp;26 || 27 || 28 || 29
+
|BBa_K266010
-
|-
+
|}</center>
-
| 30 ||<Font Color="#c0c0c0">&nbsp;1</Font> ||<Font Color="#c0c0c0">&nbsp;2</Font> ||<Font Color="#c0c0c0">&nbsp;3</Font> ||<Font Color="#c0c0c0">&nbsp;4</Font> ||<Font Color="#c0c0c0">&nbsp;5</Font> ||<Font Color="#c0c0c0">&nbsp;6</Font>  
+
 
-
|}
+
{{Template:IPN-UNAM-Mexico-footer}}
-
|}
+
-
</div>
+
-
<br><br><br><br><br><br>
+

Latest revision as of 01:29, 22 October 2009


BannerUNAM.jpg

NOTEBOOK

As summer proyect our experimental work began at july the 7th 2009 using 2008 bioparts catalog, unfortunately it didn't work properly, so we had a lot of trouble to take out DNA and transform into E. coli. For this reason we had to delay lab work and request the 2009 catalog.

Month-icon.pngProtocols

Month-icon.pngJuly

Month-icon.pngAugust

Month-icon.pngSeptember

Month-icon.pngOctober

Month-icon.pngSafety

Our synthetic construction consists of 29 bioparts, which needed 12 ligations and different strategies to make it functional as is described below:


Number Biobrick Restriction
2 BBa_R0079 S & P
3 BBa_F1610 X & P
4 BBa_K091146 S & P
5 BBa_K093005 X &
6 BBa_K081016 X & P
7 BBa_EC840 X & P
8 BBa_K081009 X & P
9 BBa_R0051 S &P
10 BBa_J06800 X & P
11 BBa_Q04121 X & P
12 BBa_B0034 S & P
13 BBa_C0079 E & S
14 BBa_C0179 E & S
15 BBa_B0015 E & X
16 BBa_K081018 E & S
17 BBa_K116640 X & P
23 BBa_S03154 X & P
24 BBa_k145201 E & S


Ligation Nickname Biobrick
4-23 Ciencias I BBa_K226005
13-15 Ciencias II BBa_K266002
Ciencias I-7 Ciencias III BBa_K266006
24-17 Ciencias IV BBa_K266001
2-3 Ciencias V BBa_K266000
J231000-11 Ciencias VI BBa_K266008
Amplicon - Ciencias II Ciencias VII BBa_K266003
Ciencias VI - 12 Ciencias VIII BBa_K266009
Ciencias III - 19 Ciencias IX BBa_K266006
J23100 – Ciencias VII Ciencias X BBa_K266004
Ciencias III - Ciencias V Ciencias XI BBa_K266007
Ciencias X - Ciencias IV Ciencias XII BBa_K266010

Banner footer UNAM2.jpg

Retrieved from "http://2009.igem.org/Team:IPN-UNAM-Mexico/Notebook"