Team:UNIPV-Pavia/Notebook/Week3Sep

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(New page: = Week from September 14th, to September 20th, 2009 = <html> <table width="100%"> <tr> <td align="left" width="50%"> <a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Sep"> <im...)
(→September, 20th)
 
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{{UNIPV-Pavia/Month}}
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__NOTOC__
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<div>
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<html><a name="week_start"></a></html>
= Week from September 14th, to September 20th, 2009 =
= Week from September 14th, to September 20th, 2009 =
<html>
<html>
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<tr>
<tr>
<td align="left" width="50%">
<td align="left" width="50%">
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Sep">
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Sep#week_start">
  <img src="https://2009.igem.org/Special:FilePath?file=Left_arrow.png"/>
  <img src="https://2009.igem.org/Special:FilePath?file=Left_arrow.png"/>
</a>
</a>
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Sep">Previous Week</a>
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Sep#week_start">Previous Week</a>
</td>
</td>
<td align="right" width="50%">  
<td align="right" width="50%">  
-
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week4Sep">Next Week</a>
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week4Sep#week_start">Next Week</a>
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week4Sep">
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week4Sep#week_start">
  <img src="https://2009.igem.org/Special:FilePath?file=Right_arrow.png"/>
  <img src="https://2009.igem.org/Special:FilePath?file=Right_arrow.png"/>
</a>
</a>
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== <html><font class="dayw_style">September, 14th</font></html> ==
== <html><font class="dayw_style">September, 14th</font></html> ==
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*Cloning
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*Wiki updating
<div align="right">
<div align="right">
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== <html><font class="dayw_style">September, 15th</font></html> ==
== <html><font class="dayw_style">September, 15th</font></html> ==
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*Fermentation experiment setup
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*Wiki updating
<div align="right">
<div align="right">
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== <html><font class="dayw_style">September, 16th</font></html> ==
== <html><font class="dayw_style">September, 16th</font></html> ==
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*Fermentation experiment
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*Wiki updating
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== <html><font class="dayw_style">September, 17th</font></html> ==
== <html><font class="dayw_style">September, 17th</font></html> ==
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''Experiment with Tecan F200''
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*<html><a href="http://aimed11.unipv.it/iGEM2009/TestTECANInfiniteF200/A1A2A7 TEST 17-09-09.pdf" target="_blank">Download Protocol</a></html>
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*<html><a href="http://aimed11.unipv.it/iGEM2009/TestTECANInfiniteF200/Ethanol test TEST 17-09-09.pdf" target="_blank">Download Protocol</a></html>
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===== pH sensor =====
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*Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
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**RBS33
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**A2
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**K116002 (from now on it will be indicated only as pNhaA)
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*Overnight incubation of these three cultures at 37°C, 220 rpm.
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== <html><font class="dayw_style">September, 18th</font></html> ==
== <html><font class="dayw_style">September, 18th</font></html> ==
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===== pH sensor =====
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''1st experiment''
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*We put 50 ul into 5 ml LBK + Amp pH 6,6 falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four hours and a half at 37°C, 220 rpm.
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*OD measure of the three falcons with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02.
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*We diluted:
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**RBS into:
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*** LBK + Amp pH 5,5
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*** LBK + Amp pH 6,6
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*** LBK + Amp pH 7,5
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*** LBK + Amp pH 8,5
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**pNhaA into:
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*** LBK + Amp pH 5,5
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*** LBK + Amp pH 6,6
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*** LBK + Amp pH 7,5
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*** LBK + Amp pH 8,5
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**A2 into:
 +
*** LBK + Amp pH 5,5
 +
*** LBK + Amp pH 6,6
 +
*** LBK + Amp pH 7,5
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*** LBK + Amp pH 8,5
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*We started this 6 hours' experiment that performed absorbance and fluorescence measures (on three different wells) for each sample every 5 minutes.
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</div>
</div>
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== <html><font class="dayw_style">September, 19th</font></html> ==
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*Wiki updating
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<div align="right">
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[[#top|Top]]
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</div>
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== <html><font class="dayw_style">September, 20th</font></html> ==
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*Cloning
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*Wiki updating
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<div align="right">
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[[#top|Top]]
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</div>
<html>
<html>
<hr/>
<hr/>
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<tr>
<tr>
<td align="left" width="50%">
<td align="left" width="50%">
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Sep">
+
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Sep#week_start">
  <img src="https://2009.igem.org/Special:FilePath?file=Left_arrow.png"/>
  <img src="https://2009.igem.org/Special:FilePath?file=Left_arrow.png"/>
</a>
</a>
-
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Sep">Previous Week</a>
+
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Sep#week_start">Previous Week</a>
</td>
</td>
<td align="right" width="50%">  
<td align="right" width="50%">  
-
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week3Sep">Next Week</a>
+
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week4Sep#week_start">Next Week</a>
-
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week3Sep">
+
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week4Sep#week_start">
  <img src="https://2009.igem.org/Special:FilePath?file=Right_arrow.png"/>
  <img src="https://2009.igem.org/Special:FilePath?file=Right_arrow.png"/>
</a>
</a>

Latest revision as of 23:38, 21 October 2009

EthanolPVanimation.gif

December 2008
M T W T F S S
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8 9 10 11 12 13 14
15 16 17 18 19 20 21
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29 30 31
March 2009
M T W T F S S
1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
April 2009
M T W T F S S
1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May 2009
M T W T F S S
1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June 2009
M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July 2009
M T W T F S S
1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August 2009
M T W T F S S
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September 2009
M T W T F S S
1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October 2009
M T W T F S S
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November 2009
M T W T F S S
1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30

Week from September 14th, to September 20th, 2009

Previous Week Next Week

September, 14th

  • Cloning
  • Wiki updating

Top

September, 15th

  • Fermentation experiment setup
  • Wiki updating

Top

September, 16th

  • Fermentation experiment
  • Wiki updating


Top

September, 17th

Experiment with Tecan F200

pH sensor
  • Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
    • RBS33
    • A2
    • K116002 (from now on it will be indicated only as pNhaA)
  • Overnight incubation of these three cultures at 37°C, 220 rpm.


Top

September, 18th

pH sensor

1st experiment

  • We put 50 ul into 5 ml LBK + Amp pH 6,6 falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four hours and a half at 37°C, 220 rpm.
  • OD measure of the three falcons with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02.
  • We diluted:
    • RBS into:
      • LBK + Amp pH 5,5
      • LBK + Amp pH 6,6
      • LBK + Amp pH 7,5
      • LBK + Amp pH 8,5
    • pNhaA into:
      • LBK + Amp pH 5,5
      • LBK + Amp pH 6,6
      • LBK + Amp pH 7,5
      • LBK + Amp pH 8,5
    • A2 into:
      • LBK + Amp pH 5,5
      • LBK + Amp pH 6,6
      • LBK + Amp pH 7,5
      • LBK + Amp pH 8,5
  • We started this 6 hours' experiment that performed absorbance and fluorescence measures (on three different wells) for each sample every 5 minutes.


Top

September, 19th

  • Wiki updating


Top

September, 20th

  • Cloning
  • Wiki updating

Top

Previous Week Next Week
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