Team:Chiba/Notebook/Calendar/22 September 2009
From 2009.igem.org
(→Examine limit of AHL generation) |
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+ | == Transformation(1)-2 == | ||
+ | Yesterday's operation is [https://2009.igem.org/Team:Chiba/Notebook/Calendar/21_September_2009#Transformation.281.29-1 here]. | ||
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+ | |||
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+ | *Today's operation | ||
+ | We count number of colonies of each plate. | ||
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+ | |||
+ | 11:30 | ||
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+ | コロニーをつついて培養 | ||
+ | |||
+ | pCIA3-LuxR | ||
+ | |||
+ | LacZ alpha protein generator | ||
+ | |||
+ | |||
+ | |||
+ | 23:50 | ||
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+ | mCherryのコロニーができたのでつついて培養 | ||
+ | |||
+ | グリストをつついて培養 | ||
+ | |||
+ | LuxI | ||
+ | |||
+ | [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] | ||
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+ | |||
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+ | == To judge character of LuxR mutants (1)-2 == | ||
+ | Yesterday's operation is [https://2009.igem.org/Team:Chiba/Notebook/Calendar/21_September_2009 here]. | ||
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+ | *Today's operation | ||
11:00- | 11:00- | ||
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- | + | *Mutants' Location | |
<table width="300" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000"><tr> | <table width="300" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000"><tr> | ||
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== ''E''. coli Painting (2) == | == ''E''. coli Painting (2) == | ||
+ | We painted some pictures using excess culture(LuxR Mutant 1) and cultured these. | ||
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+ | '''Pictures are [https://2009.igem.org/wiki/index.php?title=Team:Chiba/Notebook/Calendar/23_September_2009 here].''' | ||
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+ | == To judge character of LuxR mutants (2)-1 == | ||
+ | We poured 1 mL of LB-Amp, Cm liquid medium in 96 deep well and added glycerol stocks. | ||
+ | |||
+ | |||
+ | 11:45- | ||
+ | |||
+ | We cultured and shook it at 37 degrees Celsius. | ||
+ | |||
+ | |||
+ | 23:20- | ||
+ | |||
+ | We transplanted E.coli by 48 pins to NC filter and cultured it. | ||
+ | |||
+ | |||
+ | |||
+ | == Examine limit of AHL generation(1)-1 == | ||
+ | 23:00 | ||
+ | |||
+ | We did prior culture(JW1908 glycerol stock, plac-LuxI, and 12.5 mL of LB-Amp). |
Latest revision as of 17:12, 25 September 2009
(21_September_2009 <|>23_September_2009)
Contents |
Transformation(1)-2
Yesterday's operation is here.
- Today's operation
We count number of colonies of each plate.
11:30
コロニーをつついて培養
pCIA3-LuxR
LacZ alpha protein generator
23:50
mCherryのコロニーができたのでつついて培養
グリストをつついて培養
LuxI
[http://partsregistry.org/Part:BBa_T9002 BBa_T9002]
To judge character of LuxR mutants (1)-2
Yesterday's operation is here.
- Today's operation
11:00-
We transplanted E.coli by 48 pins to NC filter and cultured it.
22:30
We transplanted E.coli, which has been cultured on NC filter, to solid medium which contains each concentration of AHL.
AHL concentration is : 0, 10, and 1000 nM
We decided this time is T=0 and observed condition of fluorescence every 30 min.
- Mutants' Location
Wild Type x 3 well | Mutant 8 x 3 well |
Mutant 1 | Mutant 9 |
Mutant 2 | Mutant 10 |
Mutant 3 | Mutant 11 |
Mutant 4 | Mutant L1 |
Mutant 5 | Mutant L2 |
Mutant 6 | Negative Control |
Mutant 7 | (Nothing) |
Pictures are here.
observed condition of fluorescence every 30 min
22:35 Start
23:05
23:35
24:05
24:35
25:05
25:35
26:05
26:35
E. coli Painting (2)
We painted some pictures using excess culture(LuxR Mutant 1) and cultured these.
Pictures are here.
To judge character of LuxR mutants (2)-1
We poured 1 mL of LB-Amp, Cm liquid medium in 96 deep well and added glycerol stocks.
11:45-
We cultured and shook it at 37 degrees Celsius.
23:20-
We transplanted E.coli by 48 pins to NC filter and cultured it.
Examine limit of AHL generation(1)-1
23:00
We did prior culture(JW1908 glycerol stock, plac-LuxI, and 12.5 mL of LB-Amp).