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Virginia Commonwealth/11 June 2009
From 2009.igem.org
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===Results=== | ===Results=== | ||
*Note these were plated Wednesday evening with two groups splitting the work. | *Note these were plated Wednesday evening with two groups splitting the work. | ||
| - | |||
*Group 1: Craig, Clay, Afton plates | *Group 1: Craig, Clay, Afton plates | ||
*Observations on the Controls | *Observations on the Controls | ||
| - | {| cellpadding=" | + | {| cellpadding="5" border="1" padding="5" |
|+ | |+ | ||
|- | |- | ||
| Line 59: | Line 58: | ||
|- | |- | ||
! NEB10β (-) | ! NEB10β (-) | ||
| - | | Kan || 3 colonies || | + | | Kan || 3 colonies || |
| - | + | |} | |
| - | + | * Growth on Kan could have resulted from a non-homogeneous medium with very small sections of the plate being able to sustain life. Or there was degradation of the Kan-selective medium. | |
*Observations on the Colonies | *Observations on the Colonies | ||
| - | + | {| cellpadding="5" border="1" padding="5" | |
| - | {| cellpadding=" | + | |
|+ | |+ | ||
|- | |- | ||
| Line 74: | Line 72: | ||
|- | |- | ||
! E0240 | ! E0240 | ||
| - | | Amp || | + | | Amp || 100s of colonies || no color || |
| + | |- | ||
| + | ! J23101 | ||
| + | | Amp || 1000s of colonies || Pink in color. || | ||
|- | |- | ||
! J23113 | ! J23113 | ||
| - | | Amp || | + | | Amp || 100s of colonies || n/a || |
|- | |- | ||
! J23116 | ! J23116 | ||
| Amp || 100s of colonies || n/a || | | Amp || 100s of colonies || n/a || | ||
|- | |- | ||
| - | ! | + | ! J23102 |
| - | | Amp || | + | | Amp ||10s of colonies || Pink in color.|| |
|- | |- | ||
! R0040 | ! R0040 | ||
| Line 92: | Line 93: | ||
*Observations on the Colonies | *Observations on the Colonies | ||
| - | {| cellpadding=" | + | {| cellpadding="5" border="1" padding="5" |
|+ | |+ | ||
|- | |- | ||
| Line 98: | Line 99: | ||
|- | |- | ||
! R0011 | ! R0011 | ||
| - | | Amp || | + | | Amp || 10s of colonies || n/a || |
|- | |- | ||
! J23100 | ! J23100 | ||
| - | | Amp || 10s of colonies || | + | | Amp || 10s of colonies || Slightly pink || |
|- | |- | ||
! J23103 | ! J23103 | ||
| - | | Amp || | + | | Amp || low 100s of colonies || Pink in color || |
|- | |- | ||
! J23104 | ! J23104 | ||
| - | | Amp || | + | | Amp || 100s of colonies || n/a || |
|- | |- | ||
! J23105 | ! J23105 | ||
| Amp || 100s of colonies || n/a || | | Amp || 100s of colonies || n/a || | ||
|- | |- | ||
| - | ! | + | ! J23107 |
| - | | Amp || | + | | Amp ||1000s of colonies || n/a || |
|- | |- | ||
| - | ! | + | ! J23110 |
| - | | Amp || | + | | Amp ||1000s of colonies || n/a || |
|} | |} | ||
| - | * | + | *Problems: |
| + | **Part J23106 was taken from well 18D, when it actually is located in well 18O. | ||
| + | **Part Q04511 was actually taken from well 18D | ||
| + | **Part R0011 was plated twice. The plate with the most colonies was used for growth overnight. | ||
[[User:MandM|MandM]] 22:52, 11 June 2009 (UTC) | [[User:MandM|MandM]] 22:52, 11 June 2009 (UTC) | ||
| Line 125: | Line 129: | ||
===Tasks=== | ===Tasks=== | ||
| - | |||
* Literature Discussion | * Literature Discussion | ||
* Discussion of iGEM ideas | * Discussion of iGEM ideas | ||
| + | ---- | ||
====Wetlab==== | ====Wetlab==== | ||
| - | * Picking Colonies to be grown for Mini-preps and two Maxi-preps | + | * Picking Transformed Colonies to be grown overnight for Mini-preps (5mL) and two Maxi-preps(125mL) |
| - | + | ||
[[User:MandM|MandM]] 22:55, 11 June 2009 (UTC) | [[User:MandM|MandM]] 22:55, 11 June 2009 (UTC) | ||
Latest revision as of 15:48, 5 August 2009
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Contents |
Thursday 11 June 2009
Results
- Note these were plated Wednesday evening with two groups splitting the work.
- Group 1: Craig, Clay, Afton plates
- Observations on the Controls
| Control | Plate Type | Observation | |
|---|---|---|---|
| NEB10β (+) | LB | lawn | |
| NEB10β (-) | Cm | no growth | |
| NEB10β (-) | Amp | no growth | |
| NEB10β (-) | Kan | 3 colonies |
- Growth on Kan could have resulted from a non-homogeneous medium with very small sections of the plate being able to sustain life. Or there was degradation of the Kan-selective medium.
- Observations on the Colonies
| Part No. | Plate Type | Colonies | Other Observations | |
|---|---|---|---|---|
| pSB 3K3 | Kan | 10s of colonies | n/a | |
| E0240 | Amp | 100s of colonies | no color | |
| J23101 | Amp | 1000s of colonies | Pink in color. | |
| J23113 | Amp | 100s of colonies | n/a | |
| J23116 | Amp | 100s of colonies | n/a | |
| J23102 | Amp | 10s of colonies | Pink in color. | |
| R0040 | Amp | 10s of colonies | n/a |
- Group 2: Adam, Kevin and Maria Plates
- Observations on the Colonies
| Part No. | Plate Type | Colonies | Other Observations | |
|---|---|---|---|---|
| R0011 | Amp | 10s of colonies | n/a | |
| J23100 | Amp | 10s of colonies | Slightly pink | |
| J23103 | Amp | low 100s of colonies | Pink in color | |
| J23104 | Amp | 100s of colonies | n/a | |
| J23105 | Amp | 100s of colonies | n/a | |
| J23107 | Amp | 1000s of colonies | n/a | |
| J23110 | Amp | 1000s of colonies | n/a |
- Problems:
- Part J23106 was taken from well 18D, when it actually is located in well 18O.
- Part Q04511 was actually taken from well 18D
- Part R0011 was plated twice. The plate with the most colonies was used for growth overnight.
MandM 22:52, 11 June 2009 (UTC)
Tasks
- Literature Discussion
- Discussion of iGEM ideas
Wetlab
- Picking Transformed Colonies to be grown overnight for Mini-preps (5mL) and two Maxi-preps(125mL)
MandM 22:55, 11 June 2009 (UTC)
