Latest revision as of 15:44, 23 September 2009

Calibration protocols (CA)

Section	Assay	Overview and Aims
CA1:Calibration Curves	TOP10 Growth	* To produce a calibration curve to aid in the normalising of absorbance values. The relation of absorbance reading to number of cells varies with different cell strains. We are therefore doing one for Top-10.
CA2:External GFP fluorescence	External fluorescence	* To relate how the fluorescence observed can be correlated with number of purified GFP molecules
CA3:Internal GFP fluorescence	Intracellular fluorescence	* To relate how various intracellular concentrations of GFP leads to different observed fluorescences. This allows us to relate measured fluorescence from cells to actual GFP number

@@ Line 1: / Line 1: @@
 {{Imperial/09/TemplateTop}}
+=Calibration protocols (CA)=
 {| style="color:#CCC; background-color:#325d97;" cellpadding="6" cellspacing="0" border="3"
 ! Section
@@ Line 10: / Line 11: @@
 |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
 | <b>CA2</b>:External GFP fluorescence
-| [[Team:| Link]]
+| [[Imperial_College_London/Wetlab/Protocols/FluorEx| External fluorescence]]
-| * T <br>
+| * To relate how the fluorescence observed can be correlated with number of purified GFP molecules <br>
 |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
 | <b>CA3</b>:Internal GFP fluorescence
-| [[Team:| Link]]
+| [[Imperial_College_London/Wetlab/Protocols/FluorIn| Intracellular fluorescence]]
-| * T <br>
+| * To relate how various intracellular concentrations of GFP leads to different observed fluorescences. This allows us to relate measured fluorescence from cells to actual GFP number <br>
 |}
 {{Imperial/09/TemplateBottom}}