Team:UC Davis/Approach

From 2009.igem.org

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=Our Approach=
=Our Approach=
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We are going to break down gluten inside the stomach, because gliadin does not prompt an immune system response until absorption in the small intestines. Another worry is our delivery system taking residence somewhere outside the stomach, so we designed a pH inducible apoptosis system.  This is achieved through sensing the change of pH from the stomach to the small intestine, a difference of about four to five.
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Gliadin induces an immune system response when it is absorbed into the blood stream through the small intestine. We plan to take advantage of this by breaking down gliadin in the stomach before it reaches the small intestine. We have developed a secretion system to release an enzyme that can break down gliadin. Also, in order to prevent our delivery system from taking residence anywhere outside of the stomach, we plan to incorporate the difference in pH between the stomach and small intestine with a pH-inducible apoptosis system. By adopting these two systems, we will create a pH-moderated secretion system.
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Unlike other suggested treatments requiring the consumption of capsules or tablets, our method of using ''Escherichia coli'' is more cost- and time-efficient.
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Unlike other suggested treatments requiring the consumption of capsules or tablets, our method of using ''E. coli'' as our delivery system is more cost- and time-efficient.
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==Secretion==
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==Induced Secretion==
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*Gene sequence for secretion system
*Gene sequence for secretion system
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[[https://2009.igem.org/Team:UC_Davis/About_Us About Us]] [[https://2009.igem.org/Team:UC_Davis/Celiac Celiac Disease]] [[https://2009.igem.org/Team:UC_Davis/Treatments Current Treatment]] [[https://2009.igem.org/Team:UC_Davis/Approach Our Approach]] [[https://2009.igem.org/Team:UC_Davis/pH_Sensor pH Sensor]] [[https://2009.igem.org/Team:UC_Davis/pH_Sensor/parts pH Sensor Parts]] [[https://2009.igem.org/Team:UC_Davis/pH_Sensor/promoters pH Promoters]] [[https://2009.igem.org/Team:UC_Davis/Secretion/parts Secretion Parts]]
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| [https://2009.igem.org/Team:UC_Davis Home] | [https://2009.igem.org/Team:UC_Davis/About_Us About Us] | [https://2009.igem.org/Team:UC_Davis/Celiac Celiac Disease] | [https://2009.igem.org/Team:UC_Davis/Treatments Current Treatment] | [https://2009.igem.org/Team:UC_Davis/Approach Our Approach] | [https://2009.igem.org/Team:UC_Davis/pH_Sensor pH Sensor] | [https://2009.igem.org/Team:UC_Davis/pH_Sensor/parts pH Sensor Parts] | [https://2009.igem.org/Team:UC_Davis/pH_Sensor/promoters pH Promoters] | [https://2009.igem.org/Team:UC_Davis/Secretion/parts Secretion Parts] |

Latest revision as of 22:41, 23 September 2009

Our Approach

Gliadin induces an immune system response when it is absorbed into the blood stream through the small intestine. We plan to take advantage of this by breaking down gliadin in the stomach before it reaches the small intestine. We have developed a secretion system to release an enzyme that can break down gliadin. Also, in order to prevent our delivery system from taking residence anywhere outside of the stomach, we plan to incorporate the difference in pH between the stomach and small intestine with a pH-inducible apoptosis system. By adopting these two systems, we will create a pH-moderated secretion system.

Unlike other suggested treatments requiring the consumption of capsules or tablets, our method of using E. coli as our delivery system is more cost- and time-efficient.

Secretion

  • Gene sequence for secretion system
  • Adding Secretion
  • Assay for measuring protein localization and activity

Sensing pH and Inducing Cell Death

  • Finding an appropriate biological pH sensor
  • Wiring the pH sensor into our system
  • Assay for measuring the behavior of the pH sensor


| Home | About Us | Celiac Disease | Current Treatment | Our Approach | pH Sensor | pH Sensor Parts | pH Promoters | Secretion Parts |

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