Team:UC Davis/Parts 1

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<div style="text-align: center;"><b style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><a
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href="https://2009.igem.org/Team:UC_Davis/homepage2"><img alt=""
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src="https://static.igem.org/mediawiki/2009/a/a4/UCDAVIS_PIC3.png"
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style="border: 0px solid ; width: 83px; height: 36px;"></a> </big></big></big></big></span></b><a
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href="https://2009.igem.org/Team:UC_Davis/About_Us1"><b
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style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><img
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alt="" src="https://static.igem.org/mediawiki/2009/d/d1/UCDAVIS_PIC4.png"
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big>
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</big></big></big></big></span></b><b style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><a
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href="https://2009.igem.org/Team:UC_Davis/Project1"><img alt=""
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style="border: 0px solid ; width: 78px; height: 36px;"></a> </big></big></big></big></span></b><b
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><a
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href="https://2009.igem.org/Team:UC_Davis/Notebook1"><img alt=""
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src="https://static.igem.org/mediawiki/2009/2/2f/UCDAVIS_PIC5.png"
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style="border: 0px solid ; width: 81px; height: 36px;"></a> </big></big></big></big></span></b><b
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style="color: rgb(255, 255, 153);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><a
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href="https://2009.igem.org/Team:UC_Davis/Part1"><img alt=""
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src="https://static.igem.org/mediawiki/2009/a/a6/UCDAVIS_PIC6.png"
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style="border: 0px solid ; width: 78px; height: 37px;"></a>&nbsp;<a
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href="https://2009.igem.org/Team:UC_Davis/Contact1"><img alt=""
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src="https://static.igem.org/mediawiki/2009/1/1d/UCDAVIS_PIC7.png"
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style="border: 0px solid ; width: 83px; height: 37px;"></a></big></big></big></big></span></b><b
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style="color: rgb(0, 0, 0);"><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><small>
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</small></big></big></big></big></span></b><b
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style="color: rgb(0, 0, 0);">&nbsp;&nbsp; </b>
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<hr style="width: 100%; height: 2px;">
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<div style="text-align: left;"><b
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style="color: rgb(0, 0, 0); text-decoration: underline;">Parts:</b><b
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style="color: rgb(0, 0, 0);">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<br>
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</b>
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<div style="text-align: left;"><b style="color: rgb(0, 0, 0);">Parts
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related to
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Secretion:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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Parts related to the biological pH sensor:</b><br>
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</div>
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<b style="color: rgb(0, 0, 0);">Protines:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp;
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Promoters:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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Others:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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Proteins:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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Promoters:<br>
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</b><a href="#INPNC">INPNC&nbsp;</a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp;
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<a href="#LacI">LacI&nbsp;&nbsp;</a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="#SS">SS:signal
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sequence&nbsp;</a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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ChvI&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="#ChvI_promoter">ChvI promoter</a><br>
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<a href="#OmpA">OmpA&nbsp;&nbsp;</a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="#His">6-His-Tag</a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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ChvG&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="#KatA">KatA promoter</a><br>
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<a href="#Luciferase">Luciferase&nbsp;</a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="#RBS">RBS</a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="#aopB">aopB promoter</a><br>
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<a href="#GFP">GFP</a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="#Terminator">Terminator</a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="#PhoA">phoA
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promoter</a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp;&nbsp; <br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="#impA">impA promoter</a><br>
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<hr style="width: 100%; height: 2px;">
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<p class="MsoNormal"
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style="margin-bottom: 0.0001pt; line-height: normal;"><a name="INPNC"></a><span
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style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">INPNC:
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Ice-nucleation protein (INP) from Pseudomonas Syringae was suggested to
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be used
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for display of foreign proteins on the surface of <i>E.coli</i>(7).Furthermore,
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researches have shown that an INP derivative constituting the N-and
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C-terminal
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domains can and has been used to display foreign proteins on the
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surface of <i>E.coli</i>(9).
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<i><br>
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</i></span></p>
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<p class="MsoNormal"
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style="margin-bottom: 0.0001pt; line-height: normal;"><span
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style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><i>Note:
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A study has shown that "Ice- nucleation Protein (INP), an outer
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membrane protein from Pseudomonas syringae, is able to catalyze the ice
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crystal
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formation of supercooled water.</i>In our project we are intending to
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harness
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and make use of this feature by fusing a specific protein to it. <i>We
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have
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modified this protein to Biobrick standard, Tom Knights Standard. </i><o:p></o:p></span></p>
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<p class="MsoNormal" style="line-height: normal;"><a name="OmpA"></a><span
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style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">OmpA:
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OmpA is one of the proteins on the outer membrane of <i>E.coli</i>
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(13). OmpA
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has been found to be useful as utilizable fusion part that can fuse our
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protein
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to and display on the surface of <i>E.coli</i>. This part has already
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been
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documented on the parts registry; however, it has not been tested via
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fusion
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with a target protein linked with a cleavable signal sequence.<o:p></o:p></span><br>
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<i><span
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style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">We
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have modified this protein to
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Biobrick standard, Tom Knights Standard. </span></i><span
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style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
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<p class="MsoNormal" style="line-height: normal;"><i><span
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style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">Note:</span></i><span
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style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> “<i>It
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has remained essentially unknown how proteins of
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Escherichia coli outer membrane are sorted and incorporated into this
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membrane”
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(10)</i><o:p></o:p></span><i><span
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style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><br>
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For more information go to:</span></i><span
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style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> <a
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href="http://partsregistry.org/wiki/index.php?title=Part:BBa_J36836"><i><span
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style="color: blue;">http://partsregistry.org/wiki/index.php?title=Part:BBa_J36836</span></i></a><br
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style="">
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<!--[if !supportLineBreakNewLine]--><br style="">
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<!--[endif]--><o:p></o:p></span></p>
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<p class="MsoNormal" style="line-height: normal;"><a name="RBS"></a><span
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style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">RBS:
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Ribosome Binding site number 32 (BBa_J61132) from the registry is being
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used in
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our secretion system.<o:p></o:p></span><br>
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<i><span
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style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">For
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more information go to:</span></i><span
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style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> <a
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href="http://partsregistry.org/wiki/index.php/Part:BBa_J61132"><i><span
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style="color: blue;">http://partsregistry.org/wiki/index.php/Part:BBa_J61132</span></i></a><o:p></o:p></span></p>
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<p class="MsoNormal" style="line-height: normal;"><a name="Terminator"></a><span
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style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">Terminator:
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We are using BBa_B0015, a double terminator, as our terminator in both
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our
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secretion and pH system.<o:p></o:p></span><br>
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<i><span
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style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">For
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more information go to:</span></i><a name="GFP:"></a><span
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style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> <a
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href="http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015"><i><span
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style="color: blue;">http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015</span></i></a><o:p></o:p></span></p>
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<p class="MsoNormal" style="line-height: normal;"><a name="GFP"></a><span
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style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">GFP:
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We are using Green Fluorescent Protein as a reporter that also serves
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as a
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small protein in testing our secretion system.<o:p></o:p></span></p>
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<p class="MsoNormal" style="line-height: normal;"><a name="Luciferase"></a><span
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style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">Luciferase:
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Luciferase is a firefly protein that also fluoresces, so it serves as a
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reporter as well as a testable large protein.<i><br>
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More can be found in<a name="LacI:"></a>:</i><o:p></o:p></span></p>
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<p class="MsoNormal" style="line-height: normal;"><a name="LacI"></a><span
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style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">LacI:
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One inducible Promoter which was found in the part registry.<i><br>
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More can be found in: </i><a
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href="http://partsregistry.org/wiki/index.php?title=Part:BBa_R0010"><i><span
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-
style="color: blue;">http://partsregistry.org/wiki/index.php?title=Part:BBa_R0010</span></i></a><br>
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-
<br>
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<a name="SS"></a>SS: This signal sequence, when placed between INPNC,
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contains a
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cleavable site that allows the target fusion protein to ‘secrete’ from
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INPNC.&nbsp; We will do the same with OmpA.&nbsp; <o:p></o:p></span><br>
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<i><span
+
-
style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">We
+
-
have modified this protein to
+
-
Biobrick standard, Tom Knights Standard. </span></i><span
+
-
style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
+
-
<p class="MsoNormal" style="line-height: normal;"><a name="His"></a><span
+
-
style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">6-His
+
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Tag: The 6-Histidine Tag serves as a tag for Western Blotting if our
+
-
fluorescent reporters are not expressed as highly as we would like.<o:p></o:p></span><br>
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-
<i><span
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-
style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">Note:
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We are using this tag, just in
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-
case if the GFP or Luciferase does not work under a plate reader.</span></i><span
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-
style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span></p>
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-
<p class="MsoNormal" style="line-height: normal;"><span
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-
style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><a
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name="ChvI_promoter"></a>ChvI promoter: Gene fusion studies
+
-
confirmed that ChvI gene was induced by acidic conditions (1). Also, it
+
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has
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been known to implicate in virulence (1). This gene is one of the
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-
candidates to
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-
be use in our biological pH sensor as a promoter.<br style="">
+
-
<!--[if !supportLineBreakNewLine]--><br style="">
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<!--[endif]--><o:p></o:p></span></p>
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<p class="MsoNormal" style="line-height: normal;"><a name="KatA"></a><span
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-
style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">KatA
+
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promoter :This Chromosomal gene is located on the linear chromosome (2)
+
-
and it
+
-
seems to be induced under an acidic environment as well as being
+
-
involved in
+
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the <i>Agrobacterium tumorigenesis</i> (2).Research has suggested that
+
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ChvG is
+
-
needed for "responsiveness of&nbsp; gene expression to low pH "(2).
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-
This gene has become a candidate to complete our pH sensor device from
+
-
this
+
-
evidence.<br style="">
+
-
<!--[if !supportLineBreakNewLine]--><br style="">
+
-
<!--[endif]--><o:p></o:p></span></p>
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-
<p class="MsoNormal" style="line-height: normal;"><a name="aopB"></a><span
+
-
style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">aopB
+
-
promoter: This Chromosomal gene located on the circular chromosome (2)
+
-
encodes an
+
-
outer member protein exposed on the bacterial cell surface (2). Also,
+
-
ChvG was
+
-
shown to be absolutely required for this gene expression (2)It seems to
+
-
get
+
-
induced under an acidic environment as well as being involved in the <i>Agrobacterium</i>
+
-
<i>tumorigenesis </i>(2). Therefore, we have chosen this gene to be
+
-
one of our
+
-
candidates to complete our pH sensor device.<o:p></o:p></span></p>
+
-
<p class="MsoNormal" style="line-height: normal;"><a name="PhoA"></a><span
+
-
style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">PhoA
+
-
promoter: There has been a suggestion that ChvI can activate AP
+
-
activity by
+
-
activating transcription of this gene, PhoA (3). Therefore, this gene
+
-
has
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become one of our candidates to complete our pH sensor device.<o:p></o:p></span></p>
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<a name="impA"></a><span
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style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">impA
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promoter: Gene
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fusion studies confirmed that impA genes was induced by acidic
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conditions (1),
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therefore, this is one of our candidates to complete our pH sensor
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device.</span>
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<hr style="width: 100%; height: 2px;"><small style="font-weight: bold;"><span
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style="font-size: 18pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span></small></div>
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</div>
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</body>
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</html>
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Latest revision as of 21:40, 25 September 2009

Retrieved from "http://2009.igem.org/Team:UC_Davis/Parts_1"