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Team:UC Davis/Project1/Finding ph sensor
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| - | style="background: white none repeat scroll 0%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left; text-decoration: underline;"><big><big><b><span | + | style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left; text-decoration: underline;"><big><big><b><span |
style="font-size: 13pt; font-family: "Times New Roman","serif"; color: black;">Finding | style="font-size: 13pt; font-family: "Times New Roman","serif"; color: black;">Finding | ||
an appropriate biological pH sensor:</span></b></big></big></p> | an appropriate biological pH sensor:</span></b></big></big></p> | ||
<p class="MsoNormal" | <p class="MsoNormal" | ||
| - | style="background: white none repeat scroll 0%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><b><span | + | style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><b><span |
style="font-size: 13pt; font-family: "Times New Roman","serif"; color: black;">Why | style="font-size: 13pt; font-family: "Times New Roman","serif"; color: black;">Why | ||
is there a need for a pH sensor?<o:p></o:p></span></b></p> | is there a need for a pH sensor?<o:p></o:p></span></b></p> | ||
| - | |||
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style="font-size: 12pt; font-family: "Times New Roman","serif"; color: black;"> We | style="font-size: 12pt; font-family: "Times New Roman","serif"; color: black;"> We | ||
would like our probiotic organism to confine itself to the stomach. | would like our probiotic organism to confine itself to the stomach. | ||
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confinement by pH | confinement by pH | ||
levels, and then add it to the registry!<o:p></o:p></span></p> | levels, and then add it to the registry!<o:p></o:p></span></p> | ||
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style="font-size: 13pt; font-family: "Times New Roman","serif"; color: black;">Finding | style="font-size: 13pt; font-family: "Times New Roman","serif"; color: black;">Finding | ||
an appropriate biological pH sensor:</span></b><b><span | an appropriate biological pH sensor:</span></b><b><span | ||
style="font-size: 13pt; font-family: "Times New Roman","serif";"><o:p></o:p></span></b></p> | style="font-size: 13pt; font-family: "Times New Roman","serif";"><o:p></o:p></span></b></p> | ||
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| - | style="background: white none repeat scroll 0%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><span | + | style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><span |
style="font-size: 12pt; font-family: "Times New Roman","serif"; color: black;"> | style="font-size: 12pt; font-family: "Times New Roman","serif"; color: black;"> | ||
We have decided to design our pH sensor by adopting one of the pH | We have decided to design our pH sensor by adopting one of the pH | ||
sensors of <i>Agrobacterium | sensors of <i>Agrobacterium | ||
| - | + | tumefaciens</i>. The ChvG/ChvI two-component system of <i>A.tumefaciens</i> | |
is | is | ||
| - | suspected to sense extracellular pH changes | + | suspected to sense extracellular pH changes and relay this |
environmental | environmental | ||
| - | signal to numerous virulence related promoters (2). <o:p></o:p></span></p | + | signal to numerous virulence-related promoters(2). <o:p></o:p></span></p> |
| - | + | ||
<p class="MsoNormal" | <p class="MsoNormal" | ||
| - | style="background: white none repeat scroll 0%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><span | + | style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><span |
style="font-size: 12pt; font-family: "Times New Roman","serif"; color: black;"> | style="font-size: 12pt; font-family: "Times New Roman","serif"; color: black;"> | ||
| - | <i>For more information regarding | + | <i>For more information regarding </i></span><span |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">< | + | style="font-style: italic;">ChvG/ChvI two component system</span><span |
| - | href="ChvG.html">< | + | style="font-size: 12pt; font-family: "Times New Roman","serif"; color: black;"><i>, |
| - | < | + | go to <a href="https://2009.igem.org/Team:UC_Davis/Project1/ChvG.html">ChvG</a>, |
| + | <a href="https://2009.igem.org/Team:UC_Davis/ChvI1">ChvI</a>, <a | ||
| + | href="https://2009.igem.org/Team:UC_Davis/ChvG_ChvI">ChvG--->ChvI</a><br> | ||
| + | </i></span></p> | ||
| + | <hr style="width: 100%; height: 2px;"> | ||
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| - | style="font-size: 12pt; font-family: "Times New Roman","serif"; color: black;"> | + | <span |
| - | + | style="font-size: 12pt; font-family: "Times New Roman","serif"; color: black;"></span><span | |
| - | + | style="font-size: 12pt; font-family: "Times New Roman","serif";"><a | |
| - | < | + | href="https://2009.igem.org/Team:UC_Davis/ChvG_ChvI"><i><span |
| + | style="color: blue;"></span></i></a><o:p></o:p></span></p> | ||
<p class="MsoNormal" | <p class="MsoNormal" | ||
| - | style="background: white none repeat scroll 0%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;" | + | style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><i><u><span |
| - | + | style="font-size: 12pt; font-family: "Times New Roman","serif"; color: blue;"></span></u></i><span | |
| - | + | ||
| - | style="font-size: 12pt; font-family: "Times New Roman","serif"; color: blue;"> | + | |
| - | + | ||
style="font-size: 12pt; font-family: "Times New Roman","serif"; color: black;"><o:p></o:p></span></p> | style="font-size: 12pt; font-family: "Times New Roman","serif"; color: black;"><o:p></o:p></span></p> | ||
</div> | </div> | ||
</body> | </body> | ||
</html> | </html> | ||
Latest revision as of 02:26, 22 October 2009
Finding an appropriate biological pH sensor:
Why
is there a need for a pH sensor?
We
would like our probiotic organism to confine itself to the stomach.
Since low
pH is a hallmark of the stomach, it seems reasonable to assume that
linking the
production of a suicide gene to a dramatic increase in pH (to values
found in
the intestine) should allow us to sequester our organism to the
stomach. There
are no pH sensors available on the registry of biological parts. So, we
will
engineer a device to sense extracellular pH changes and design it so
that it
can be used to regulate the expression of any target gene in
confinement by pH
levels, and then add it to the registry!
Finding
an appropriate biological pH sensor:
We have decided to design our pH sensor by adopting one of the pH
sensors of Agrobacterium
tumefaciens. The ChvG/ChvI two-component system of A.tumefaciens
is
suspected to sense extracellular pH changes and relay this
environmental
signal to numerous virulence-related promoters(2).
For more information regarding ChvG/ChvI two component system,
go to ChvG,
ChvI, ChvG--->ChvI
