Team:Chiba/Notebook/Calendar/30 September 2009

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([[Team:Chiba/Notebook/Calendar/29_September_2009|29_September_2009]] <|>[[Team:Chiba/Notebook/Calendar/1_October_2009|1_October_2009]])
([[Team:Chiba/Notebook/Calendar/29_September_2009|29_September_2009]] <|>[[Team:Chiba/Notebook/Calendar/1_October_2009|1_October_2009]])
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== Transformation ==
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Yesterday's operation is [https://2009.igem.org/Team:Chiba/Notebook/Calendar/29_September_2009 here].
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*Today's operation
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10:30-
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Culture at 37 degrees Celsius
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20:00-
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Mini Prep.
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20:40-
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Digestion Test
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20:56-
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At 37 degrees Celsius
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21:45-
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Gel electro...(135 V, 25 min)
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== PCR ==
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Yesterday's operation is [https://2009.igem.org/Team:Chiba/Notebook/Calendar/29_September_2009 here].
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11:15-
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Gel electro...(135 V, 27 min)
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12:10
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Took a picture
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== Cloning ==
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=== Digestion ===
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Yesterday's operation is [https://2009.igem.org/Team:Chiba/Notebook/Calendar/29_September_2009 here].
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12:37-
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Gel electro...(100 V, 40 min)
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13:20
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into ethyl bromide
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13:35
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Took a picture and cut the gel.
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Then we added 2 mL of ADB Buffer and kept it at 37 degrees Celsius.
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=== DNA Clean & Concentrator ===
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We eluted it 50 uL of dH<sub>2</sub>O.
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=== SAP treatment ===
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=== DNA Clean & Concentrator ===
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We eluted it 50 uL of dH<sub>2</sub>O.
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=== Gel electrophoresis ===
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17:32
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Electrophoresis
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18:15
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Took a picture
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=== Ligation ===
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18:30
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@Room Temperture
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21:30
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Transformation
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21:50
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@37 degrees Celsius
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23:10
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Plating

Latest revision as of 23:26, 21 October 2009

>Go to the Notebook page

(29_September_2009 <|>1_October_2009)


Contents

Transformation

Yesterday's operation is here.


  • Today's operation

10:30-

Culture at 37 degrees Celsius


20:00-

Mini Prep.


20:40-

Digestion Test


20:56-

At 37 degrees Celsius


21:45-

Gel electro...(135 V, 25 min)


PCR

Yesterday's operation is here.

11:15-

Gel electro...(135 V, 27 min)


12:10

Took a picture

Cloning

Digestion

Yesterday's operation is here.

12:37-

Gel electro...(100 V, 40 min)


13:20

into ethyl bromide


13:35

Took a picture and cut the gel.

Then we added 2 mL of ADB Buffer and kept it at 37 degrees Celsius.

DNA Clean & Concentrator

We eluted it 50 uL of dH2O.

SAP treatment

DNA Clean & Concentrator

We eluted it 50 uL of dH2O.

Gel electrophoresis

17:32

Electrophoresis


18:15

Took a picture

Ligation

18:30

@Room Temperture


21:30

Transformation


21:50

@37 degrees Celsius


23:10

Plating

Retrieved from "http://2009.igem.org/Team:Chiba/Notebook/Calendar/30_September_2009"