August/9 August 2009

From 2009.igem.org

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1.colony check
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[https://2009.igem.org/Team:Osaka/NOTES back to NOTES]
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  ( sample No.)  (no. of colonies)
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        9                50<
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        24                0
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        29                0
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        30                0
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        31                8
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        32                2
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        9                1
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2.digestion and ligation<br>
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digestion with restriction enzyme<br>
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'''K204024'''
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<table border="1" Frame="box">
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<tr><th colspan="2">Vector</th><th colspan="2">Insert</th></tr>
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<tr><td>terminater(1-23L)</td><td>6</td><td>No. 14-A </td><td>12</td></tr>
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<tr><td>EcoRI</td><td>1</td><td>EcoRI</td><td>1</td><tr>
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<tr><td>XbaI</td><td>1</td><td>SpeI</td><td>1</td><tr>
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<tr><td> No.2</td><td>2</td><td>No.2</td><td>2</td></tr>
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<tr><td>dH<sub>2</sub>O</td><td>10</td><td>dH<sub>2</sub>O</td><td>4</td></tr>
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<tr><td>total</td><td>20uL</td><td>total</td><td>20uL</td></tr>
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</table>
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'''K204029'''
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<table border="1" Frame="box">
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<tr><th colspan="2">Vector</th><th colspan="2">Insert</th></tr>
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<tr><td>2-18F</td><td>6</td><td>2-16</td><td>10</td></tr>
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<tr><td>EcoRI</td><td>1</td><td>EcoRI</td><td>1</td><tr>
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<tr><td>XbaI</td><td>1</td><td>SpeI</td><td>1</td><tr>
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<tr><td> No.2</td><td>2</td><td>No.2</td><td>2</td></tr>
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<tr><td>dH<sub>2</sub>O</td><td>10</td><td>dH<sub>2</sub>O</td><td>6</td></tr>
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<tr><td>total</td><td>20uL</td><td>total</td><td>20uL</td></tr>
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</table>
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'''K204030'''
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<table border="1" Frame="box">
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<tr><th colspan="2">Vector</th><th colspan="2">Insert</th></tr>
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<tr><td>2-18F</td><td>10</td><td>1-19B</td><td>6</td></tr>
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<tr><td>SpeI</td><td>1</td><td>XbaI</td><td>1</td><tr>
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<tr><td>PstI</td><td>1</td><td>PstI</td><td>1</td><tr>
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<tr><td> No.2 </td><td>2</td><td>No.2</td><td>2</td></tr>
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<tr><td>dH<sub>2</sub>O</td><td>6</td><td>dH<sub>2</sub>O</td><td>10</td></tr>
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<tr><td>total</td><td>20uL</td><td>total</td><td>20uL</td></tr>
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</table>
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↓<br>
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37degree 3hr<br>
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↓<br>
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Agarose Gel Electro-phoresis<br>
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<br>
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[[Image:Osaka090909.jpg]]<br>
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No. 24 , 29 , 30 , 10-D , 10-E , ladder
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↓<br>
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16 degree O/N<br>
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3.Min prep<br>
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Result of Nanodrop concentration check
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  (sample No.)      (concentration)
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      1-1D                27.5
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      10-D                85.1
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      10-E                117.2
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4.transformation<br>
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ligation sample No. 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40A ,40B
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sample No. 24 , 29 , 30
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5.digestion and ligation<br>
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digestion with restriction enzyme<br>
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'''K204041'''
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<table border="1" Frame="box">
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<tr><th colspan="2">Vector</th><th colspan="2">Insert</th></tr>
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<tr><td>1-1D</td><td>12</td><td>1-12M</td><td>8</td></tr>
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<tr><td>SpeI</td><td>1</td><td>XbaI</td><td>1</td><tr>
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<tr><td>PstI</td><td>1</td><td>PstI</td><td>1</td><tr>
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<tr><td> No.2 </td><td>2</td><td>No.2</td><td>2</td></tr>
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<tr><td>dH<sub>2</sub>O</td><td>4</td><td>dH<sub>2</sub>O</td><td>8</td></tr>
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<tr><td>total</td><td>20uL</td><td>total</td><td>20uL</td></tr>
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</table>
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'''K204042'''
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<table border="1" Frame="box">
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<tr><th colspan="2">Vector</th><th colspan="2">Insert</th></tr>
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<tr><td>1-1D</td><td>12</td><td>1-8I</td><td>8</td></tr>
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<tr><td>SpeI</td><td>1</td><td>XbaI</td><td>1</td><tr>
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<tr><td>PstI</td><td>1</td><td>PstI</td><td>1</td><tr>
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<tr><td> No.2 </td><td>2</td><td>No.2</td><td>2</td></tr>
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<tr><td>dH<sub>2</sub>O</td><td>4</td><td>dH<sub>2</sub>O</td><td>8</td></tr>
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<tr><td>total</td><td>20uL</td><td>total</td><td>20uL</td></tr>
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</table>
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↓<br>
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37degree 3hr<br>
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↓<br>
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Agarose Gel Electro-phoresis<br>
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<br>
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[[Image:Osaka090909-2.jpg]]<br>
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No. 41 , 42 , ladder
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↓<br>
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16 degree O/N<br>
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Latest revision as of 07:38, 12 October 2009

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