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Imperial College London/Notebook/30 September 2009
From 2009.igem.org
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| + | ==Testing team== | ||
| + | ===Harvard GFP=== | ||
| + | *Problems: | ||
| + | **Fluorescence was saturated for our positive control | ||
| + | <!--**The shifted sample (SS) does not seem to show a lag phase when compared to SC2 (Constant at 37°C). We were expecting 28°C to be less optimal than 37°C. The lack of lag phase (even if brief) could suggest that 28°C is not that sub-optimal as a temperature for the system tested.--> | ||
| + | *Conclusions: | ||
| + | **Fluorescence for the shifted sample (SS) shows increase of fluorescence overtime indicating that the temperature sensitive promoter (pLambda cI) is activated when shifted to 37°C | ||
| + | **However we could not determined whether the shifted sample reached the fluorescence level of the positive control as fluorescence readings were saturated on the fluorometer. | ||
| + | *The non shifted sample (kept at 28°C) was constant at fluorescence levels similar to the blanks. This suggests that the promoter repression is tight at 28°C. | ||
| + | *Blanks were constant | ||
Latest revision as of 14:29, 1 October 2009
Nuri: -Did lacI/lactose diagram - Will read papers and continue CSS tutorial
Testing team
Harvard GFP
- Problems:
- Fluorescence was saturated for our positive control
- Conclusions:
- Fluorescence for the shifted sample (SS) shows increase of fluorescence overtime indicating that the temperature sensitive promoter (pLambda cI) is activated when shifted to 37°C
- However we could not determined whether the shifted sample reached the fluorescence level of the positive control as fluorescence readings were saturated on the fluorometer.
- The non shifted sample (kept at 28°C) was constant at fluorescence levels similar to the blanks. This suggests that the promoter repression is tight at 28°C.
- Blanks were constant
