Team:Washington/Secretion

From 2009.igem.org

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= I DO NOT KNOW HOW TO REMOVE THE CONTENTS THING FROM THE TOP BUT I THINK WE SHOULD TURN IT INTO OUR SIDE BAR =
 
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=EXAMPLE PAGE=
 
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== BANNER GOES HERE ==
 
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[[Image:Placeholder.JPG|150px|left|caption]]'''SMALL GRAPHIC FROM MAIN PAGE AND DESCRIPTION OF GOAL ex. "The Secretion System easily recognizes secretion tag on A Favorite Protein and exports that protein to the extracellular milieu."'''
 
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This is the background and design section.  "There have been many secretion systems documented for Gram-negative bacteria.  We chose X system because it has been described to have N characteristics [Reference] and it has been previously shown to secrete proteins [References]."
 
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== More Detailed Graphic Goes Here ==
 
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'''For example parts list / plasmid of the system, or whatever graphic technically describes this portion.'''
 
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*[Graphic] Part A has these characteristics.  This part spans the inner and outer membranes and acts as a pore, with a mechanism to selectively recognize the secretion tag described in the [[Target]] section.
 
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*[Graphic] Part B has these characteristics.  This part is embedded in the outer membrane and contributes to secretion in a way that has not yet been defined.
 
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*Etc.
 
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== Construction (and Data) Section ==
 
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Short sentence explaining experiment.  "In order to test whether we were getting expression of the display protein, we first performed a Western blot analysis with an anti-his detection reagent.  We incubated overnight cultures and HERE IS WHERE YOU DESCRIBE THE EXPERIMENT."
 
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'''DATA FIGURE''' - And explanation of the data figure.  "We found that..."
 
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CONCLUSION about the data.  "This demonstrates that our proteins are being expressed inside of the cell."
 
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'''Repeat as needed for more data.'''
 
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== At the end of the page, be clear about what was generated and where we stopped, and what work (if any) remains to be done. ==
 
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=== "How do I use this part in my synthetic biology project" link to go to User Guide page ===
 
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=== link to [[Next_Page]]
 
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== REFERENCES ==
 

Latest revision as of 04:52, 1 October 2009