Team:Freiburg bioware

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<big><font size="5">Team Freiburg Bioware</font></big>
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<h1 style="border-bottom: none;">Team Freiburg Bioware</h1>
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<br />
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The first German team ever to participate in iGEM is back again and
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<p> With three years of experience in iGEM and after last year's second place the iGEM Team Freiburg is highly motivated &nbsp;to deliver
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after last year's second place we're highly motivated &nbsp;to make
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some good piece of synthetic biology in 2009. In this year we want to
some good piece of synthetic biology in 2009. In this year we want to
create an universal restriction enzyme to facilitate labwork
create an universal restriction enzyme to facilitate labwork
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<br />
<br />
We're looking forward to meeting you on this year's jamboree!<br />
We're looking forward to meeting you on this year's jamboree!<br />
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<h2 style="border-bottom: none;" class="art-PostHeaderIcon-wrapper"> <span
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      <td><b>Universal Endonuclease &ndash; Cutting
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Edge Technology</b>
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      <p>Gene technology is driven by the use of restriction
 +
endonucleases.
 +
Yet, constraints of limited sequence length and variation recognized by
 +
available restriction enzymes pose a major roadblock for synthetic
 +
biology. We developed the basis for universal restriction enzymes,
 +
primarily for routine cloning but also with potential for in vivo
 +
applications. We use a nucleotide cleavage domain fused to a binding
 +
domain, which recognizes a programmable adapter that mediates binding
 +
to DNA and thus cleavage. As adapter we use readily available modified
 +
oligonucleotides, as binding domain anticalins and as cleavage domain
 +
FokI moieties engineered for heterodimerization and activity. For
 +
cloning, this universal enzyme has merely to be mixed with the sequence
 +
specific oligonucleotide and the target DNA. Binding and release are
 +
addressed with thermocycling. Additionally, we provide concepts for in
 +
vivo applications by external adapter delivery, activity regulation by
 +
photo-switching, as well as for modifying an argonaute protein towards
 +
a DNA endonuclease.&nbsp;</p>
 +
      <p><a
 +
href="https://2009.igem.org/Team:Freiburg_bioware/Project">You
 +
can find the Highlights of our projects here</a></p>
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      </div>
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<p><b>Universal Endonuclease – Cutting Edge Technology</b></p>
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<p>Gene technology is driven by the use of restriction endonucleases. Yet, constraints of limited sequence length and variation recognized by available restriction enzymes pose a major roadblock for synthetic biology. We developed the basis for universal restriction enzymes, primarily for routine cloning but also with potential for in vivo applications. We use a nucleotide cleavage domain fused to a binding domain, which recognizes a programmable adapter that mediates binding to DNA and thus cleavage. As adapter we use readily available modified oligonucleotides, as binding domain anticalins and as cleavage domain FokI moieties engineered for heterodimerization and activity. For cloning, this universal enzyme has merely to be mixed with the sequence specific oligonucleotide and the target DNA. Binding and release are addressed with thermocycling. Additionally, we provide concepts for in vivo applications by external adapter delivery, activity regulation by photo-switching, as well as for modifying an argonaute protein towards a DNA endonuclease.
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<p><b>The Team</b><br />
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This year our team consists of 18 undergraduate students and 4 advisors.<br />
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<b>The Team</b><br />
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<span style="text-decoration: underline;"><img
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In 2009 our team consists of &nbsp;14 undergraduates and 4 advisors.<br />
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  style="width: 147px; height: 49px;" alt="bioss"
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<a href="https://2009.igem.org/Team:Freiburg_bioware/Team">Read
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  src="https://static.igem.org/mediawiki/2009/3/34/Freiburg09_Bioss_portlet.jpg" /></span>
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more...</a></p>
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<p><b>Bioss</b><br />
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<p><span style="font-weight: bold;"><a
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Bioss</span><br />
We want to thank our main sponsor Bioss for supporting our project.<br />
We want to thank our main sponsor Bioss for supporting our project.<br />
-
<a target="_blank" href="http://www.bioss.uni-freiburg.de">Read
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<a href="http://www.bioss.uni-freiburg.de">Read more...</a></p>
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more...</a></p>
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Latest revision as of 14:00, 24 November 2009

Artisteer

Welcome

Team Freiburg Bioware


With three years of experience in iGEM and after last year's second place the iGEM Team Freiburg is highly motivated  to deliver some good piece of synthetic biology in 2009. In this year we want to create an universal restriction enzyme to facilitate labwork and enable new techniques.

We're looking forward to meeting you on this year's jamboree!


Project Summary

Universal Endonuclease – Cutting Edge Technology

Gene technology is driven by the use of restriction endonucleases. Yet, constraints of limited sequence length and variation recognized by available restriction enzymes pose a major roadblock for synthetic biology. We developed the basis for universal restriction enzymes, primarily for routine cloning but also with potential for in vivo applications. We use a nucleotide cleavage domain fused to a binding domain, which recognizes a programmable adapter that mediates binding to DNA and thus cleavage. As adapter we use readily available modified oligonucleotides, as binding domain anticalins and as cleavage domain FokI moieties engineered for heterodimerization and activity. For cloning, this universal enzyme has merely to be mixed with the sequence specific oligonucleotide and the target DNA. Binding and release are addressed with thermocycling. Additionally, we provide concepts for in vivo applications by external adapter delivery, activity regulation by photo-switching, as well as for modifying an argonaute protein towards a DNA endonuclease. 

You can find the Highlights of our projects here

Sponsors


Sponsors

FREiGEM 2009

Team
The Team
In 2009 our team consists of  14 undergraduates and 4 advisors.
Read more...

bioss
Bioss
We want to thank our main sponsor Bioss for supporting our project.
Read more...

Visitors

 freigem09@googlemail.com

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