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Team:Chiba/Notebook/Calendar/28 September 2009
From 2009.igem.org
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| - | == Gel | + | == Gel electrophoresis == |
| + | *Sample | ||
| + | Vector(plux-GFP), V-YFP, mOrange, and sfGFP | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | 13:12 | ||
| + | |||
| + | We made gel for electrophoresis. | ||
| + | |||
| + | agar 0.14 g | ||
| + | |||
| + | TAE 20 mL | ||
| + | |||
| + | |||
| + | 14:08- | ||
| + | |||
| + | Gel electrophoresis(135 V, 27 min) | ||
| + | |||
| + | |||
| + | 14:40- | ||
| + | |||
| + | Ethyl bromideに浸ける | ||
| + | |||
| + | |||
| + | 15:05 | ||
| + | |||
| + | Took a picture | ||
| + | |||
| + | |||
| + | |||
| + | == Prepare for new gel electrophoresis == | ||
| + | 19:15 | ||
| + | |||
| + | We cultured plux-GFP with 50 mL of LB-Cm in 300 mL flask at 37 degrees Celsius. | ||
Latest revision as of 07:28, 3 October 2009
(27_September_2009 <|>29_September_2009)
Gel electrophoresis
- Sample
Vector(plux-GFP), V-YFP, mOrange, and sfGFP
13:12
We made gel for electrophoresis.
agar 0.14 g
TAE 20 mL
14:08-
Gel electrophoresis(135 V, 27 min)
14:40-
Ethyl bromideに浸ける
15:05
Took a picture
Prepare for new gel electrophoresis
19:15
We cultured plux-GFP with 50 mL of LB-Cm in 300 mL flask at 37 degrees Celsius.
