Team:Chiba/Notebook/Calendar/30 September 2009

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13:20
13:20
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Ethyl bromideに浸す
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into ethyl bromide
13:35
13:35
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Took a picture and ゲルを切り出す。
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Took a picture and cut the gel.
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Then we added 2 mL of ADB Buffer and put it in 37 degrees Celsius of 培養機
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Then we added 2 mL of ADB Buffer and kept it at 37 degrees Celsius.
=== DNA Clean & Concentrator ===
=== DNA Clean & Concentrator ===
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We 溶出ed 50 μL.
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We eluted it 50 uL of dH<sub>2</sub>O.
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=== SAP処理 ===
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*まぜ表
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15:45-
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インキュベート it at 37 degrees Celsius.
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---30 min---
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16:20
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We transferred it at 65 degrees Celsius and 失活させた
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---15 min---
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=== SAP treatment ===
=== DNA Clean & Concentrator ===
=== DNA Clean & Concentrator ===
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We 溶出ed 20 &mu;L.
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We eluted it 50 uL of dH<sub>2</sub>O.
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=== Gel electrophoresis ===
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=== Gel electro... ===
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17:32
17:32
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Electro...
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Electrophoresis
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Took a picture
Took a picture
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=== Ligation ===
=== Ligation ===

Latest revision as of 23:26, 21 October 2009

>Go to the Notebook page

(29_September_2009 <|>1_October_2009)


Contents

Transformation

Yesterday's operation is here.


  • Today's operation

10:30-

Culture at 37 degrees Celsius


20:00-

Mini Prep.


20:40-

Digestion Test


20:56-

At 37 degrees Celsius


21:45-

Gel electro...(135 V, 25 min)


PCR

Yesterday's operation is here.

11:15-

Gel electro...(135 V, 27 min)


12:10

Took a picture

Cloning

Digestion

Yesterday's operation is here.

12:37-

Gel electro...(100 V, 40 min)


13:20

into ethyl bromide


13:35

Took a picture and cut the gel.

Then we added 2 mL of ADB Buffer and kept it at 37 degrees Celsius.

DNA Clean & Concentrator

We eluted it 50 uL of dH2O.

SAP treatment

DNA Clean & Concentrator

We eluted it 50 uL of dH2O.

Gel electrophoresis

17:32

Electrophoresis


18:15

Took a picture

Ligation

18:30

@Room Temperture


21:30

Transformation


21:50

@37 degrees Celsius


23:10

Plating

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