From 2009.igem.org
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| - | == 6-15-2009 ==
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| - | Lab: LV, SH, CZ
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| - | '''SH''':
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| - | * [[Team:Heidelberg/n4tr1um#Isolation of plasmid DNA by alkaline lysis (mini and maxiprep)|Miniprep]] of GFP template plasmid, pcDNA5/FRT
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| - | {| class="wikitable" border="1"
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| - | |-
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| - | ! Nr
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| - | ! pcDNA5/FRT
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| - | ! GFP
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| - | |-
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| - | | 1
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| - | | 4,7
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| - | | 39,7
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| - | |-
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| - | | 2
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| - | | 5,9
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| - | | 14,9
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| - | |-
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| - | | 3
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| - | | 3,7
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| - | | 3,8
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| - | |-
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| - | | 4
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| - | | 5,0
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| - | | 7,8
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| - | |-
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| - | |}
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| - | * [[Team:Heidelberg/n4tr1um#Isolation of plasmid DNA by alkaline lysis (mini and maxiprep)|Maxiprep]] pcDNA/FRT 188.7ng/µL
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| - | '''LV''':
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| - | * Extraction of CMV promoter from 2008 distribution
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| - | * [[Team:Heidelberg/n4tr1um# Transformation of Bacteria|Transformation]] of DH5a cell with CMV promoter
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| - | * portzughtr
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| - |
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| - | == 6-16-2009 ==
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| - |
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| - | Lab: LV, SH, CZ
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| - | * Annealing of Oligos (JeT, cFos, Min)
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| - | * Purification of cDNA via 2% / 3% agarosegel
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| - |
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| - | * [[Team:Heidelberg/n4tr1um#Site-directed mutagenesis|Site directed mutagenesis]] of pcDNA5/FRT
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| - | * Dpn1 digestion
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| - | * Transformation of DH5a
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Latest revision as of 12:24, 24 June 2009
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