EPF-Lausanne/12 October 2009

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==Wet Lab==
==Wet Lab==
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Digestion assay (with Pst1 and SpeI) in order to check the double transformant -KO strand JRG1046.
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We checked 3 clones of each double transformant: RO1.1+BB1 and RO2.4+BB1.
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==People in the lab==
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N.B: RO1.1+BB1 clone #1 culture wasn't pink at all
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<html><center><a href="https://2009.igem.org/EPF-Lausanne/10_October_2009"><img src="https://static.igem.org/mediawiki/2009/thumb/6/61/Flèche_gauche.png/70px-Flèche_gauche.png"></a>   
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Took image of agarose gel:
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<a href="https://2009.igem.org/EPF-Lausanne/12_October_2009"><img src="https://static.igem.org/mediawiki/2009/thumb/5/5e/Fleche_droite.png/70px-Fleche_droite.png"></a></center></html>  
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[[Image:121009gel_DTjrg1046.JPG|200px|thumb|center]]
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<br><br>
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RO1.1+BB1 #3 and 6 are double transformants:
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( cut RO1 --> band of ~900bp, plasmid-900bp
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RO2 --> band of 1.8 kb, plasmid - 1.8 kb
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BB --> band of ~ 200bp, 400bp, plasmid - 600bp )
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Started protocol to clone the "simple" LovTap into an iGEM plasmid: did the PCR on LovTap with primers that contained the iGEM prefix and suffix
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==People in the lab==
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Tú, Heidi
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<html><center><a href="https://2009.igem.org/EPF-Lausanne/11_October_2009"><img src="https://static.igem.org/mediawiki/2009/thumb/6/61/Flèche_gauche.png/70px-Flèche_gauche.png"></a>   
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<a href="https://2009.igem.org/EPF-Lausanne/13_October_2009"><img src="https://static.igem.org/mediawiki/2009/thumb/5/5e/Fleche_droite.png/70px-Fleche_droite.png"></a></center></html>  
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Latest revision as of 08:50, 18 October 2009

Contents

12 October 2009





Wet Lab

Digestion assay (with Pst1 and SpeI) in order to check the double transformant -KO strand JRG1046.

We checked 3 clones of each double transformant: RO1.1+BB1 and RO2.4+BB1.

N.B: RO1.1+BB1 clone #1 culture wasn't pink at all


Took image of agarose gel:

121009gel DTjrg1046.JPG



RO1.1+BB1 #3 and 6 are double transformants:

( cut RO1 --> band of ~900bp, plasmid-900bp

RO2 --> band of 1.8 kb, plasmid - 1.8 kb

BB --> band of ~ 200bp, 400bp, plasmid - 600bp )


Started protocol to clone the "simple" LovTap into an iGEM plasmid: did the PCR on LovTap with primers that contained the iGEM prefix and suffix

People in the lab

Tú, Heidi