Team:Virginia/17 June 2009

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(New page: {{Template:VirginiaNotebook}} ==Overnight Results== *Expression vectors had colonies **J04500 **J13002 *Reporters had colonies **OFP **GFP **RFP had about half colonies which grew on the ...)
(Today)
 
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**J13002
**J13002
*Reporters had colonies
*Reporters had colonies
-
**OFP
+
**PRE OFP  
-
**GFP
+
*Knockout strain transformations
-
**RFP had about half colonies which grew on the ampicillin plate but did not fluoresce.
+
**GFP visible, in JW3469 & JW3470
 +
**RFP in JW3469 & JW3470, visible only in part of the colonies
*Serial Dilution still saturated
*Serial Dilution still saturated
 +
==Today==
==Today==
*Made broths out of plates.
*Made broths out of plates.
 +
*Digested A09-1(2)A (X,P)
 +
**LM Gel
 +
*Digested A09-2B,C (E,P)
 +
**[[Image:Vgem61709-1.jpg]]
 +
**B has insert, C doesn't appear to

Latest revision as of 15:29, 18 June 2009

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Overnight Results

  • Expression vectors had colonies
    • J04500
    • J13002
  • Reporters had colonies
    • PRE OFP
  • Knockout strain transformations
    • GFP visible, in JW3469 & JW3470
    • RFP in JW3469 & JW3470, visible only in part of the colonies
  • Serial Dilution still saturated

Today

  • Made broths out of plates.
  • Digested A09-1(2)A (X,P)
    • LM Gel
  • Digested A09-2B,C (E,P)
    • Vgem61709-1.jpg
    • B has insert, C doesn't appear to